FastQCFastQC Report
Wed 25 May 2016
SRR1295214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10650
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT350.32863849765258213No Hit
GGTATCAACGCAGAGTACTTTTTTT290.27230046948356806No Hit
TATCAACGCAGAGTACTTTTTTTTT220.20657276995305163No Hit
GTGGTATCAACGCAGAGTACATGGG170.1596244131455399No Hit
GAATGGAATGGAATGGAATGGAATG160.15023474178403756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT160.15023474178403756No Hit
ATTCCATTCCATTCCATTCCATTCC120.11267605633802817No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)