##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295214_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10650 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.418028169014086 31.0 31.0 33.0 28.0 34.0 2 31.43605633802817 31.0 31.0 34.0 28.0 34.0 3 31.666666666666668 31.0 31.0 34.0 30.0 34.0 4 35.27239436619718 37.0 35.0 37.0 33.0 37.0 5 35.40525821596244 37.0 35.0 37.0 33.0 37.0 6 35.2837558685446 37.0 35.0 37.0 33.0 37.0 7 35.23718309859155 37.0 35.0 37.0 32.0 37.0 8 35.193333333333335 37.0 35.0 37.0 32.0 37.0 9 36.8893896713615 39.0 37.0 39.0 33.0 39.0 10 36.89765258215962 39.0 37.0 39.0 33.0 39.0 11 36.98281690140845 39.0 37.0 39.0 33.0 39.0 12 36.89014084507042 39.0 37.0 39.0 33.0 39.0 13 36.747699530516435 39.0 37.0 39.0 32.0 39.0 14 38.01718309859155 40.0 38.0 41.0 33.0 41.0 15 38.01323943661972 40.0 38.0 41.0 33.0 41.0 16 38.108450704225355 40.0 38.0 41.0 33.0 41.0 17 38.095023474178404 40.0 38.0 41.0 33.0 41.0 18 38.06253521126761 40.0 38.0 41.0 33.0 41.0 19 38.15990610328638 40.0 38.0 41.0 33.0 41.0 20 38.17530516431925 40.0 38.0 41.0 33.0 41.0 21 38.140657276995306 40.0 38.0 41.0 33.0 41.0 22 38.16366197183098 40.0 38.0 41.0 33.0 41.0 23 38.082723004694834 40.0 38.0 41.0 33.0 41.0 24 38.116056338028166 40.0 38.0 41.0 33.0 41.0 25 36.9912676056338 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2536278762653481 1101 2 -1.758918774096415 1101 3 -0.6193549985732645 1101 4 -0.28152035606701986 1101 5 0.21990233435759166 1101 6 0.594307831571939 1101 7 -1.3806987003691944 1101 8 0.36431919841913896 1101 9 1.1944646604808042 1101 10 0.8303147879607522 1101 11 -1.4995057213986982 1101 12 0.20514727797087318 1101 13 -0.15025773596834568 1101 14 0.8400706225046477 1101 15 0.7546305022642841 1101 16 1.4309535404423315 1101 17 0.823460095539204 1101 18 0.9757599578770169 1101 19 -0.23841121257456166 1101 20 -1.2357751154006564 1101 21 0.15591093803352862 1101 22 -0.49672583737894627 1101 23 -1.1808166340323965 1101 24 -1.087102535571141 1101 25 -0.6997467582569925 1102 1 -0.011203633841105898 1102 2 0.013808498630858423 1102 3 0.06751368829542415 1102 4 -0.2461668207134835 1102 5 0.13909425354951566 1102 6 0.4427926800567832 1102 7 0.8213215016510134 1102 8 1.1168444509443916 1102 9 0.8459798119959601 1102 10 1.1232440808900463 1102 11 0.8439286220356408 1102 12 0.8920159648395583 1102 13 1.657823072112464 1102 14 1.1532019356359626 1102 15 1.4718022194360003 1102 16 0.5875191970079925 1102 17 0.49012676220586826 1102 18 0.5161639982810584 1102 19 0.7413867672234176 1102 20 1.2440228643973228 1102 21 0.772072554195141 1102 22 0.6446883040351992 1102 23 0.4656480124322542 1102 24 1.0240085755399733 1102 25 -0.007827566337802239 1103 1 -0.07787030050777233 1103 2 0.31380849863085913 1103 3 -0.7102640894823544 1103 4 -0.05727793182459351 1103 5 -0.08312796867270578 1103 6 -0.7572073199432126 1103 7 -0.42312294279343377 1103 8 0.9946222287221715 1103 9 1.2126464786626272 1103 10 1.145466303112265 1103 11 0.8883730664800851 1103 12 0.0475715203951097 1103 13 0.08004529433468832 1103 14 -0.3356869532529245 1103 15 0.8273577749915546 1103 16 -1.3569252474364575 1103 17 1.0234600955392068 1103 18 1.1939417760588356 1103 19 1.652497878334529 1103 20 0.25513397550843564 1103 21 1.4165169986395867 1103 22 1.0002438595907535 1103 23 1.5100924568766985 1103 24 1.3128974644288576 1103 25 2.469950211439972 1104 1 0.05546303282556053 1104 2 -1.086191501369143 1104 3 -0.04359742281568657 1104 4 -0.3906112651579292 1104 5 0.05020536466062708 1104 6 -1.290540653276551 1104 7 -1.0231229427934352 1104 8 0.6612888953888358 1104 9 -0.05402018800404562 1104 10 0.41213296977893066 1104 11 0.6883730664800893 1104 12 0.847571520395114 1104 13 1.0133786276680183 1104 14 0.06431304674707405 1104 15 -0.30597555834177825 1104 16 -0.22359191410312462 1104 17 1.3567934288725354 1104 18 0.12727510939217268 1104 19 0.45249787833453325 1104 20 0.0551339755084399 1104 21 0.28318366530625383 1104 22 0.6002438595907549 1104 23 0.7100924568766942 1104 24 1.1128974644288618 1104 25 0.0032835447733106093 1105 1 -0.28203696717443805 1105 2 0.07630849863085842 1105 3 -0.14776408948235442 1105 4 -0.12811126515792637 1105 5 -0.04146130200604148 1105 6 -2.411373986609881 1105 7 -0.6647896094601009 1105 8 -0.14704443794449418 1105 9 -0.9248535213373756 1105 10 -2.675367030221068 1105 11 -1.419960266853245 1105 12 -0.6357618129382203 1105 13 -0.9741213723319788 1105 14 0.1184797134137412 1105 15 -1.0976422250084426 1105 16 -1.5860919141031218 1105 17 -0.48903990446079604 1105 18 -2.081058223941163 1105 19 -2.085002121665468 1105 20 -0.7073660244915629 1105 21 0.25818366530625525 1105 22 0.0544105262574206 1105 23 -1.402407543123303 1105 24 -0.962102535571141 1105 25 0.24911687810664063 1106 1 0.06722773870791343 1106 2 0.33733791039556493 1106 3 -0.1808523247764704 1106 4 0.7505652054303056 1106 5 0.1717739921116035 1106 6 -1.047403398374584 1106 7 -0.46626019769539795 1106 8 -1.0367503202974362 1106 9 -1.4579417566314916 1106 10 -0.8702199713975389 1106 11 -1.280254384500303 1106 12 -2.0034088717617493 1106 13 -0.9042684311555078 1106 14 -0.48078499246861384 1106 15 0.2038283632268545 1106 16 -0.02359191410312178 1106 17 0.470518919068617 1106 18 0.323353540764721 1106 19 -0.9710315334301711 1106 20 -1.674277789197447 1106 21 -1.9991692758702158 1106 22 -1.4272071208014054 1106 23 -0.148731072535071 1106 24 0.14819158207591698 1106 25 0.11308746634193767 1107 1 -1.407036967174438 1107 2 -0.7986915013691416 1107 3 -0.14776408948235442 1107 4 0.49688873484207363 1107 5 -0.9164613020060415 1107 6 0.7136260133901189 1107 7 0.3977103905398991 1107 8 -0.022044437944494177 1107 9 0.7626464786626244 1107 10 0.07463296977893208 1107 11 0.580039733146755 1107 12 0.4267381870617797 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0.8883730664800851 2207 12 1.2475715203951125 2207 13 1.8800452943346855 2207 14 1.6643130467470755 2207 15 1.2273577749915603 2207 16 0.4430747525635468 2207 17 0.890126762205874 2207 18 1.3272751093921684 2207 19 1.3191645450012004 2207 20 1.921800642175107 2207 21 2.149850331972921 2207 22 1.8669105262574206 2207 23 1.843425790210027 2207 24 1.979564131095529 2207 25 2.8699502114399706 2208 1 0.5966395034137975 2208 2 0.6902790868661555 2208 3 0.34855943992941363 2208 4 1.1623299113126606 2208 5 -0.004696596123686447 2208 6 0.5408318957430609 2208 7 1.1219750964222541 2208 8 0.6691320326437378 2208 9 0.6597053021920374 2208 10 0.4238976756612871 2208 11 0.896216203734987 2208 12 0.46717936353236666 2208 13 1.0957315688444922 2208 14 1.5192150075313862 2208 15 0.9685342455797894 2208 16 0.6234669094262912 2208 17 -0.647128139754912 2208 18 0.558647658411779 2208 19 1.1466155253933579 2208 20 0.6198398578613791 2208 21 -1.2344633935172737 2208 22 0.16102817331624664 2208 23 1.027739515700226 2208 24 0.324662170311214 2208 25 -0.7692654748345333 2209 1 -0.5210075554097315 2209 2 -0.6626620896044351 2209 3 -1.1808523247764704 2209 4 -2.0729642063343974 2209 5 -0.7694024784766285 2209 6 -0.8121092807275261 2209 7 -0.8780249035777459 2209 8 -0.6838091438268492 2209 9 -0.6344123448667887 2209 10 -0.517278794926952 2209 11 -0.6331955609708899 2209 12 -0.1210559305852783 2209 13 -0.1395625488025658 2209 14 -0.010196757174490756 2209 15 -1.0314657544202106 2209 16 -2.200062502338419 2209 17 -1.235363433872564 2209 18 -1.794293518058808 2209 19 -1.0298550628419392 2209 20 -1.262513083315092 2209 21 -0.46975751116433173 2209 22 -1.1330894737425794 2209 23 -0.972260484299774 2209 24 -0.851808417924083 2209 25 -0.5927948865992434 2210 1 0.40546303282556195 2210 2 0.6138084986308598 2210 3 0.2897359105176456 2210 4 0.5593887348420736 2210 5 0.7835386979939614 2210 6 0.9761260133901217 2210 7 1.0102103905398963 2210 8 0.42795556205550866 2210 9 0.6626464786626229 2210 10 -0.5378670302210651 2210 11 0.45503973314675505 2210 12 1.1642381870617768 2210 13 0.5633786276680226 2210 14 1.0309797134137426 2210 15 0.8273577749915546 2210 16 0.9764080858968782 2210 17 0.023460095539206804 2210 18 0.7439417760588398 2210 19 1.2024978783345333 2210 20 1.30513397550844 2210 21 1.3831836653062553 2210 22 1.5169105262574192 2210 23 0.5100924568766985 2210 24 0.7128974644288562 2210 25 0.23661687810663778 2211 1 0.45546303282556266 2211 2 -0.13619150136914016 2211 3 0.03973591051764558 2211 4 0.7593887348420765 2211 5 1.0835386979939585 2211 6 1.076126013390116 2211 7 0.8102103905399005 2211 8 0.6279555620555044 2211 9 0.8626464786626258 2211 10 1.012132969778932 2211 11 1.1050397331467536 2211 12 -0.2857618129382189 2211 13 0.8633786276680198 2211 14 0.9309797134137412 2211 15 1.677357774991556 2211 16 1.8264080858968796 2211 17 1.4734600955392025 2211 18 0.9439417760588356 2211 19 1.152497878334529 2211 20 0.9051339755084342 2211 21 1.833183665306258 2211 22 1.0669105262574234 2211 23 1.0600924568766956 2211 24 0.11289746442886184 2211 25 1.7366168781066378 2212 1 0.602831453878192 2212 2 0.40854534073612214 2212 3 0.23710433157027566 2212 4 1.3883361032631285 2212 5 0.6888018558886984 2212 6 0.09191548707433128 2212 7 -0.5003159252495735 2212 8 0.8332187199502457 2212 9 0.9073833207678845 2212 10 0.3805540224105144 2212 11 0.5339871015678099 2212 12 0.37739608179862216 2212 13 0.3712733645101238 2212 14 -0.22691502342836145 2212 15 -0.3936948565873877 2212 16 -0.0762234930504917 2212 17 -0.018645167618693392 2212 18 -0.732374013414848 2212 19 0.3261820888608469 2212 20 -0.2501291823863028 2212 21 0.7252889284641526 2212 22 0.39322631573110556 2212 23 0.5416714042451147 2212 24 -0.7186814829395587 2212 25 0.8313537202119008 2213 1 0.008404209296148935 2213 2 0.4549849692190939 2213 3 0.8779712046352905 2213 4 0.5740946171950156 2213 5 0.8188328156410165 2213 6 0.8937730722136479 2213 7 0.8278574493634281 2213 8 0.6691320326437378 2213 9 0.07147000807439241 2213 10 1.24742708742599 2213 11 0.30798090961734204 2213 12 0.17306171647354773 2213 13 0.5074962747268472 2213 14 0.6368620663549152 2213 15 -0.1491128132437396 2213 16 0.5058198506027622 2213 17 1.058754213186262 2213 18 0.558647658411779 2213 19 0.38190964304041586 2213 20 0.972781034331966 2213 21 -1.1756398641055128 2213 22 0.5139693497868336 2213 23 0.498327750994342 2213 24 0.618779817370033 2213 25 -0.41632429836394635 2214 1 0.655463032825562 2214 2 -0.7242867394643788 2214 3 0.14687876766050323 2214 4 0.09510302055635833 2214 5 0.6787767932320534 2214 6 0.8237450610091699 2214 7 1.0911627714922787 2214 8 -0.12918729508734828 2214 9 -0.08259161657547054 2214 10 -0.4164384587924985 2214 11 0.43123020933722955 2214 12 0.16185723468083069 2214 13 1.451473865763262 2214 14 -0.4499726675386384 2214 15 -0.06788032024653745 2214 16 -0.4521633426745524 2214 17 0.5853648574439632 2214 18 -0.3489153667983089 2214 19 -0.29035926452261407 2214 20 0.09322921360367786 2214 21 0.45461223673482465 2214 22 0.1526248119717053 2214 23 0.052949599733842945 2214 24 1.1986117501431437 2214 25 0.7937597352494947 2215 1 0.4173677947303247 2215 2 -0.9147629299405686 2215 3 -0.3293117085299748 2215 4 0.7141506396039787 2215 5 -0.08312796867270578 2215 6 0.4427926800567832 2215 7 1.0911627714922787 2215 8 -0.510139676039735 2215 9 0.20312266913881416 2215 10 0.7264186840646474 2215 11 0.907420685527704 2215 12 0.6856667584903491 2215 13 0.3086167229061161 2215 14 0.2166939991280259 2215 15 -1.2583565107227273 2215 16 -0.16644905696026768 2215 17 0.15679342887253966 2215 18 -0.253677271560214 2215 19 -0.19512116928451917 2215 20 -0.5258184054439425 2215 21 -0.02157823945564985 2215 22 0.7716724310193257 2215 23 0.5767591235433613 2215 24 0.3414688930002896 2215 25 0.22233116382092533 2216 1 -0.2778703005077716 2216 2 -0.4861915013691416 2216 3 -0.3769307561490223 2216 4 -1.0572779318245935 2216 5 -0.48312796867271146 2216 6 -0.357207319943214 2216 7 -1.3564562761267709 2216 8 -0.9387111046111585 2216 9 0.7459798119959586 2216 10 -3.121200363554401 2216 11 -2.044960266853245 2216 12 -1.4857618129382217 2216 13 -1.0532880389986445 2216 14 -3.135686953252929 2216 15 -2.0393088916751125 2216 16 -1.4235919141031204 2216 17 -2.6432065711274646 2216 18 -1.6727248906078316 2216 19 -0.9475021216654653 2216 20 -0.14486602449156294 2216 21 -1.7168163346937462 2216 22 -0.8664228070759137 2216 23 -0.5565742097899715 2216 24 -0.28710253557114385 2216 25 -1.463383121893358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 0.0 12 5.0 13 4.0 14 10.0 15 8.0 16 26.0 17 25.0 18 23.0 19 20.0 20 32.0 21 31.0 22 26.0 23 32.0 24 44.0 25 40.0 26 50.0 27 73.0 28 79.0 29 95.0 30 137.0 31 163.0 32 226.0 33 307.0 34 471.0 35 684.0 36 1137.0 37 1958.0 38 4942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29801822109515 21.733821733821735 12.801728186343572 24.16643185873955 2 15.267605633802816 22.0 39.81220657276995 22.92018779342723 3 17.3443515823082 26.59404638933233 29.702319466616583 26.359282561742887 4 12.140845070422534 18.0 38.7887323943662 31.070422535211268 5 13.806706114398423 36.72395980088288 35.587489433643285 13.88184465107542 6 30.788732394366196 39.248826291079816 16.95774647887324 13.004694835680752 7 25.80281690140845 32.06572769953052 24.47887323943662 17.652582159624412 8 23.83098591549296 36.7887323943662 21.812206572769956 17.568075117370892 9 24.63849765258216 16.56338028169014 22.619718309859156 36.178403755868544 10 14.967136150234742 29.737089201877936 34.056338028169016 21.23943661971831 11 33.04225352112676 25.417840375586852 23.624413145539904 17.91549295774648 12 20.508967978213917 26.950887407268286 32.28472157010048 20.255423044417316 13 26.634109691960933 24.65251690458302 26.22088655146506 22.492486851990982 14 20.291079812206572 23.173708920187792 28.488262910798124 28.046948356807512 15 22.356807511737088 30.413145539906104 24.647887323943664 22.582159624413144 16 21.71830985915493 31.2018779342723 26.4037558685446 20.67605633802817 17 20.272300469483568 30.356807511737088 28.816901408450708 20.553990610328636 18 20.197183098591548 29.971830985915492 29.83098591549296 20.0 19 21.305164319248824 28.507042253521124 29.370892018779344 20.816901408450704 20 22.779342723004696 27.943661971830984 29.051643192488264 20.225352112676056 21 22.92018779342723 28.488262910798124 28.497652582159628 20.093896713615024 22 21.87793427230047 28.4037558685446 29.446009389671364 20.272300469483568 23 21.699530516431924 28.422535211267608 29.408450704225352 20.469483568075116 24 21.36150234741784 27.84037558685446 29.342723004694836 21.455399061032864 25 21.36150234741784 27.943661971830984 28.845070422535212 21.84976525821596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 3.0 2 3.0 3 3.0 4 3.0 5 3.0 6 8.5 7 14.0 8 14.0 9 14.0 10 24.0 11 34.0 12 34.0 13 34.0 14 58.0 15 82.0 16 82.0 17 82.0 18 125.5 19 169.0 20 169.0 21 169.0 22 301.5 23 434.0 24 434.0 25 434.0 26 536.0 27 638.0 28 638.0 29 638.0 30 778.0 31 918.0 32 918.0 33 918.0 34 1024.0 35 1130.0 36 1130.0 37 1130.0 38 1218.5 39 1307.0 40 1307.0 41 1307.0 42 1322.5 43 1338.0 44 1338.0 45 1338.0 46 1271.0 47 1204.0 48 1204.0 49 1204.0 50 1173.5 51 1143.0 52 1143.0 53 1143.0 54 991.5 55 840.0 56 840.0 57 840.0 58 715.0 59 590.0 60 590.0 61 590.0 62 491.5 63 393.0 64 393.0 65 393.0 66 310.5 67 228.0 68 228.0 69 228.0 70 168.5 71 109.0 72 109.0 73 109.0 74 79.5 75 50.0 76 50.0 77 50.0 78 31.5 79 13.0 80 13.0 81 13.0 82 11.5 83 10.0 84 10.0 85 10.0 86 5.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028169014084507043 2 0.0 3 0.009389671361502348 4 0.0 5 0.028169014084507043 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.009389671361502348 13 0.018779342723004695 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 10650.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.55399061032864 #Duplication Level Percentage of deduplicated Percentage of total 1 98.25926286103277 94.87323943661971 2 1.4100943304483127 2.7230046948356805 3 0.15559661577360692 0.4507042253521127 4 0.048623942429252165 0.18779342723004694 5 0.009724788485850433 0.046948356807511735 6 0.009724788485850433 0.056338028169014086 7 0.019449576971700865 0.13145539906103285 8 0.019449576971700865 0.15023474178403756 9 0.0 0.0 >10 0.06807351940095302 1.380281690140845 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 35 0.32863849765258213 No Hit GGTATCAACGCAGAGTACTTTTTTT 29 0.27230046948356806 No Hit TATCAACGCAGAGTACTTTTTTTTT 22 0.20657276995305163 No Hit GTGGTATCAACGCAGAGTACATGGG 17 0.1596244131455399 No Hit GAATGGAATGGAATGGAATGGAATG 16 0.15023474178403756 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 16 0.15023474178403756 No Hit ATTCCATTCCATTCCATTCCATTCC 12 0.11267605633802817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE