##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295213_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6359 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.257115898726216 31.0 30.0 33.0 26.0 34.0 2 30.489070608586257 31.0 30.0 34.0 26.0 34.0 3 30.40116370498506 31.0 30.0 34.0 26.0 34.0 4 33.426010378990405 35.0 33.0 37.0 27.0 37.0 5 33.975153325994654 35.0 35.0 37.0 28.0 37.0 6 34.07422550715521 37.0 35.0 37.0 29.0 37.0 7 34.011165277559364 37.0 35.0 37.0 29.0 37.0 8 34.00707658436861 37.0 35.0 37.0 28.0 37.0 9 34.95738323635792 39.0 35.0 39.0 27.0 39.0 10 34.99512501965718 39.0 35.0 39.0 27.0 39.0 11 35.48435288567385 39.0 35.0 39.0 28.0 39.0 12 35.46595376631546 39.0 35.0 39.0 27.0 39.0 13 35.45856266708602 39.0 35.0 39.0 27.0 39.0 14 36.10206007233842 39.0 36.0 41.0 27.0 41.0 15 36.55071552130838 40.0 36.0 41.0 27.0 41.0 16 36.53876395659695 40.0 36.0 41.0 27.0 41.0 17 36.21276930334958 39.0 36.0 41.0 27.0 41.0 18 36.104576191225036 39.0 36.0 41.0 27.0 41.0 19 36.51486082717408 40.0 36.0 41.0 27.0 41.0 20 36.59899355244535 40.0 36.0 41.0 27.0 41.0 21 36.55417518477748 40.0 36.0 41.0 27.0 41.0 22 36.41044189337946 40.0 36.0 41.0 27.0 41.0 23 36.23101116527756 39.0 36.0 41.0 27.0 41.0 24 36.57210253184463 40.0 36.0 41.0 27.0 41.0 25 35.40462336845416 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04253326039039962 1101 2 0.5047049951811964 1101 3 0.08155852084423643 1101 4 -0.09722794841842841 1101 5 0.029943028752548173 1101 6 -3.5202951369618063 1101 7 -3.00064349231015 1101 8 0.04149267839745363 1101 9 0.9095547309833023 1101 10 1.0601903387617568 1101 11 -0.7785246059055595 1101 12 -0.474074426455374 1101 13 -0.43190231285470304 1101 14 1.208410372696079 1101 15 -0.2429103524341656 1101 16 -0.5806535175582681 1101 17 2.0031679519774883 1101 18 1.984252475919142 1101 19 -2.1856207725255246 1101 20 0.5880883666597967 1101 21 -0.5654939857320898 1101 22 0.8363509946843379 1101 23 0.6425004889290591 1101 24 0.09976328785852928 1101 25 -0.6741277681753957 1102 1 1.688687106544247 1102 2 1.9739357644119622 1102 3 2.2507892900750015 1102 4 2.7873874361969584 1102 5 1.8299430287525453 1102 6 1.9566279399612725 1102 7 2.3378180461513907 1102 8 2.403031139935912 1102 9 2.440323961752533 1102 10 3.952498031069453 1102 11 2.8522446248636726 1102 12 2.9566948043138623 1102 13 3.1373284563760606 1102 14 4.108410372696078 1102 15 3.4570896475658373 1102 16 2.5270387901340357 1102 17 3.7262448750544053 1102 18 3.653483245149907 1102 19 3.7759176890129353 1102 20 3.595780674352106 1102 21 3.39604447580637 1102 22 3.6978894562227964 1102 23 4.050192796621367 1102 24 3.430532518627764 1102 25 4.218179924132301 1103 1 0.8425332603904003 1103 2 1.2047049951811957 1103 3 1.231558520844235 1103 4 1.9027720515815716 1103 5 1.4549430287525453 1103 6 -1.2452951369618077 1103 7 -0.07564349231014944 1103 8 1.4414926783974522 1103 9 0.08455473098329946 1103 10 1.6351903387617597 1103 11 1.496475394094439 1103 12 1.7259255735446288 1103 13 1.7430976871452941 1103 14 -1.6415896273039223 1103 15 1.3320896475658373 1103 16 0.21934648244172905 1103 17 2.4281679519774855 1103 18 0.00925247591914058 1103 19 2.9393792274744754 1103 20 0.5380883666597995 1103 21 2.1845060142679102 1103 22 -0.8886490053156635 1103 23 0.2425004889290605 1103 24 2.3247632878585307 1103 25 1.4008722318246072 1104 1 -1.7408000729429318 1104 2 0.2880383285145278 1104 3 -2.851774812489097 1104 4 -2.5138946150850963 1104 5 -2.6700569712474547 1104 6 -0.9536284702951434 1104 7 -0.7839768256434851 1104 8 -3.64184065493588 1104 9 -8.707111935683368 1104 10 -7.0731429945715725 1104 11 -2.628524605905561 1104 12 -1.6074077597887069 1104 13 0.034764353811958415 1104 14 -1.3082562939705866 1104 15 -1.5429103524341627 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2.5682007586769515 1107 16 2.219346482441729 1107 17 0.6920568408663712 1107 18 2.7731413648080263 1107 19 1.6477125608078111 1107 20 0.8436439222153567 1107 21 -0.2877162079543112 1107 22 1.458573216906558 1107 23 2.520278266706839 1107 24 2.310874398969645 1107 25 3.414761120713493 1108 1 0.9425332603904018 1108 2 0.10470499518119425 1108 3 0.481558520844235 1108 4 1.2027720515815687 1108 5 0.029943028752548173 1108 6 1.4797048630381937 1108 7 2.0993565076898477 1108 8 0.5414926783974536 1108 9 2.0095547309832966 1108 10 1.360190338761761 1108 11 1.7214753940944405 1108 12 0.6259255735446274 1108 13 1.2680976871452927 1108 14 -0.2915896273039209 1108 15 1.4570896475658373 1108 16 1.3193464824417305 1108 17 1.3031679519774855 1108 18 1.1842524759191377 1108 19 1.6143792274744726 1108 20 0.6880883666597981 1108 21 1.3345060142679088 1108 22 1.4363509946843394 1108 23 1.9425004889290634 1108 24 1.3997632878585335 1108 25 2.4258722318246058 1109 1 1.8425332603904003 1109 2 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5 1.9208521196616388 2212 6 1.8797048630381923 2212 7 1.708447416780757 2212 8 1.6687654056701788 2212 9 3.3913729128014793 2212 10 3.2601903387617597 2212 11 2.621475394094439 2212 12 2.9077437553628087 2212 13 2.731734050781661 2212 14 3.562955827241531 2212 15 2.820726011202204 2212 16 2.219346482441729 2212 17 3.4395315883411186 2212 18 2.7024342941009607 2212 19 2.3143792274744754 2212 20 2.4699065484779794 2212 21 0.7526878324497304 2212 22 1.2363509946843365 2212 23 2.1061368525654274 2212 24 1.8361269242221638 2212 25 0.889508595460974 2213 1 0.14253326039040104 2213 2 0.904704995181195 2213 3 1.2815585208442357 2213 4 2.6027720515815744 2213 5 1.729943028752544 2213 6 1.8797048630381923 2213 7 0.9993565076898534 2213 8 1.0414926783974536 2213 9 1.8095547309833009 2213 10 2.860190338761761 2213 11 1.9214753940944362 2213 12 1.025925573544626 2213 13 0.6680976871452913 2213 14 0.40841037269607483 2213 15 2.65708964756584 2213 16 0.9193464824417319 2213 17 3.1031679519774826 2213 18 2.1842524759191377 2213 19 -0.785620772525526 2213 20 1.9880883666598024 2213 21 -2.7654939857320926 2213 22 1.4363509946843394 2213 23 0.5425004889290577 2213 24 1.1997632878585307 2213 25 1.6258722318246086 2214 1 1.242533260390399 2214 2 1.4047049951811985 2214 3 1.5815585208442364 2214 4 2.80277205158157 2214 5 1.6299430287525425 2214 6 1.9797048630381937 2214 7 2.399356507689852 2214 8 2.2414926783974494 2214 9 3.5095547309832966 2214 10 3.360190338761761 2214 11 2.621475394094439 2214 12 1.2259255735446288 2214 13 2.6680976871452913 2214 14 3.908410372696075 2214 15 3.15708964756584 2214 16 3.3193464824417305 2214 17 4.103167951977483 2214 18 1.0842524759191434 2214 19 2.1143792274744726 2214 20 3.188088366659798 2214 21 2.134506014267913 2214 22 2.636350994684335 2214 23 2.0425004889290577 2214 24 2.299763287858532 2214 25 1.3258722318246043 2215 1 0.953644371501511 2215 2 1.093593884070085 2215 3 2.1482251875108993 2215 4 2.18054982935935 2215 5 -0.9478347490252332 2215 6 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2216 19 3.8143792274744754 2216 20 3.6630883666597995 2216 21 3.5595060142679102 2216 22 3.4863509946843365 2216 23 0.7425004889290605 2216 24 2.3247632878585307 2216 25 4.400872231824607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 4.0 4 2.0 5 1.0 6 1.0 7 0.0 8 1.0 9 2.0 10 3.0 11 8.0 12 14.0 13 23.0 14 29.0 15 33.0 16 41.0 17 40.0 18 45.0 19 26.0 20 36.0 21 39.0 22 46.0 23 46.0 24 41.0 25 45.0 26 62.0 27 66.0 28 66.0 29 95.0 30 100.0 31 137.0 32 198.0 33 220.0 34 324.0 35 494.0 36 714.0 37 1153.0 38 2191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89763779527559 21.905511811023622 13.937007874015746 23.25984251968504 2 14.862732723256547 22.404544020195647 40.407068475859894 22.325654780687916 3 18.224889450410615 26.263423878711308 29.46936197094125 26.04232469993683 4 12.101105845181674 18.26224328593997 38.183254344391784 31.45339652448657 5 13.387071281808124 37.1424055634582 35.70412517780939 13.766397976924294 6 30.039463299131807 39.889502762430936 17.75848460931334 12.312549329123915 7 25.268308080808083 32.98611111111111 23.311237373737374 18.434343434343432 8 23.04656669297553 37.363851617995266 21.846882399368585 17.742699289660617 9 24.30939226519337 17.979479084451462 21.468034727703238 36.24309392265193 10 14.716564029685772 30.20685299226275 34.043897047213015 21.032685930838465 11 32.13440605773781 25.55608140085187 23.710364410790348 18.599148130619973 12 19.64962121212121 27.320075757575758 31.818181818181817 21.21212121212121 13 26.123994320870803 23.584161539675026 26.96008834200978 23.33175579744439 14 20.628156565656568 23.05871212121212 28.83522727272727 27.477904040404038 15 22.14330808080808 30.90277777777778 23.70580808080808 23.248106060606062 16 22.267781107080904 31.225358776218258 25.737265415549597 20.769594701151238 17 20.492579728449638 30.186296179349544 28.733817492895486 20.587306599305336 18 20.088369891115672 30.282468044816156 29.288306769764873 20.3408552943033 19 20.69455406471981 29.29755327545383 29.171270718232044 20.836621941594316 20 22.867055669452768 28.74940861062924 28.355149030121428 20.028386689796562 21 23.72159090909091 28.708964646464647 26.90972222222222 20.65972222222222 22 20.779425686336385 29.56768696749763 28.794572420321867 20.858314925844116 23 21.40041002996373 29.395994322662037 29.159438574357356 20.044157073016873 24 22.376518857503548 28.215243806217455 28.34148650781127 21.06675082846773 25 22.025875670558538 29.851688229725465 26.775007888923952 21.34742821079205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 14.0 2 15.5 3 17.0 4 17.0 5 17.0 6 16.5 7 16.0 8 16.0 9 16.0 10 17.5 11 19.0 12 19.0 13 19.0 14 37.5 15 56.0 16 56.0 17 56.0 18 90.0 19 124.0 20 124.0 21 124.0 22 177.5 23 231.0 24 231.0 25 231.0 26 301.5 27 372.0 28 372.0 29 372.0 30 465.0 31 558.0 32 558.0 33 558.0 34 619.0 35 680.0 36 680.0 37 680.0 38 745.5 39 811.0 40 811.0 41 811.0 42 774.5 43 738.0 44 738.0 45 738.0 46 730.0 47 722.0 48 722.0 49 722.0 50 677.5 51 633.0 52 633.0 53 633.0 54 574.5 55 516.0 56 516.0 57 516.0 58 448.5 59 381.0 60 381.0 61 381.0 62 309.0 63 237.0 64 237.0 65 237.0 66 180.0 67 123.0 68 123.0 69 123.0 70 95.0 71 67.0 72 67.0 73 67.0 74 47.0 75 27.0 76 27.0 77 27.0 78 19.0 79 11.0 80 11.0 81 11.0 82 7.5 83 4.0 84 4.0 85 4.0 86 3.0 87 2.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14153168737222832 2 0.33024060386853277 3 0.42459506211668496 4 0.45604654819940244 5 0.5032237773234786 6 0.3774178329926089 7 0.3616920899512502 8 0.3774178329926089 9 0.3774178329926089 10 0.4088693190753263 11 0.31451486082717406 12 0.3616920899512502 13 0.31451486082717406 14 0.3616920899512502 15 0.3616920899512502 16 0.28306337474445664 17 0.3931435760339676 18 0.34596634690989153 19 0.3774178329926089 20 0.28306337474445664 21 0.3616920899512502 22 0.33024060386853277 23 0.28306337474445664 24 0.34596634690989153 25 0.33024060386853277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6359.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.50605441107092 #Duplication Level Percentage of deduplicated Percentage of total 1 99.09003831417624 97.60968705771347 2 0.6704980842911877 1.320962415474131 3 0.11174968071519795 0.33024060386853277 4 0.015964240102171137 0.06290297216543482 5 0.06385696040868455 0.31451486082717406 6 0.015964240102171137 0.09435445824815222 7 0.0 0.0 8 0.015964240102171137 0.12580594433086964 9 0.015964240102171137 0.14153168737222832 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCATTCCATTCCATTCCATTCC 9 0.14153168737222832 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 8 0.12580594433086964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE