FastQCFastQC Report
Wed 25 May 2016
SRR1295213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTT180.28306337474445664No Hit
TATCAACGCAGAGTACTTTTTTTTT180.28306337474445664No Hit
GTATCAACGCAGAGTACTTTTTTTT160.2516118886617393No Hit
ATTCCATTCCATTCCATTCCATTCC120.18870891649630445No Hit
ACGCAGAGTACTTTTTTTTTTTTTT120.18870891649630445No Hit
GTACTTTTTTTTTTTTTTTTTTTTT90.14153168737222832No Hit
GTGGTATCAACGCAGAGTACATGGG70.11008020128951093No Hit
GAATGGAATGGAATGGAATGGAATG70.11008020128951093No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)