##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6359 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.401478219845888 31.0 31.0 33.0 28.0 34.0 2 31.469413429784556 31.0 31.0 34.0 28.0 34.0 3 31.67557792105677 31.0 31.0 34.0 28.0 34.0 4 35.24547884887561 37.0 35.0 37.0 33.0 37.0 5 35.31530114797924 37.0 35.0 37.0 33.0 37.0 6 35.249095769775124 37.0 35.0 37.0 32.0 37.0 7 35.14278974681554 37.0 35.0 37.0 32.0 37.0 8 35.10143104261677 37.0 35.0 37.0 32.0 37.0 9 36.871363421921686 39.0 37.0 39.0 33.0 39.0 10 36.825286994810504 39.0 37.0 39.0 33.0 39.0 11 36.916968076741625 39.0 37.0 39.0 33.0 39.0 12 36.82921843057085 39.0 37.0 39.0 33.0 39.0 13 36.71111810033024 39.0 37.0 39.0 32.0 39.0 14 38.00298789117786 40.0 38.0 41.0 33.0 41.0 15 38.055983645227236 40.0 38.0 41.0 33.0 41.0 16 38.10551973580752 40.0 38.0 41.0 33.0 41.0 17 38.117314043088534 40.0 38.0 41.0 33.0 41.0 18 38.04654819940242 40.0 38.0 41.0 33.0 41.0 19 38.148293756880015 40.0 38.0 41.0 33.0 41.0 20 38.030822456361065 40.0 38.0 41.0 32.0 41.0 21 38.07894323006762 40.0 38.0 41.0 33.0 41.0 22 38.04434659537663 40.0 38.0 41.0 33.0 41.0 23 38.01839911935839 40.0 38.0 41.0 32.0 41.0 24 38.109293914137446 40.0 38.0 41.0 33.0 41.0 25 37.01682654505425 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47639388829865936 1101 2 0.5498501058024843 1101 3 0.937880285499336 1101 4 0.6546245641483779 1101 5 0.5439182070134407 1101 6 0.291268475792279 1101 7 0.8290570540570528 1101 8 0.398635006968334 1101 9 1.8377117591403334 1101 10 1.4264452919214747 1101 11 1.6632522239665093 1101 12 1.577218636742451 1101 13 2.046802447992924 1101 14 1.907460440793777 1101 15 1.097378987855187 1101 16 1.7098206907730855 1101 17 0.7713934654410721 1101 18 1.4218372456467634 1101 19 1.4344076558362389 1101 20 1.9692960478674593 1101 21 2.344032158317873 1101 22 2.1252482966768653 1101 23 2.036870977347178 1101 24 1.9382190120285472 1101 25 2.1711255235064755 1102 1 0.653316965221741 1102 2 1.472927028879404 1102 3 1.0532649008839492 1102 4 -0.3453754358516221 1102 5 -0.7176202545250163 1102 6 0.5374223219461243 1102 7 0.8598262848262834 1102 8 1.3140196223529443 1102 9 1.906942528371097 1102 10 0.9956760611522384 1102 11 1.4478676085818947 1102 12 1.2541417136655326 1102 13 2.085263986454464 1102 14 1.853614286947625 1102 15 2.358917449393644 1102 16 2.3944360753884695 1102 17 1.2560088500564603 1102 18 2.22952955333907 1102 19 1.9113307327593176 1102 20 2.3077575863289965 1102 21 2.2286475429332597 1102 22 1.802171373599947 1102 23 2.16764020811641 1102 24 1.7843728581823939 1102 25 3.3788178311987807 1103 1 -1.6736061117013392 1103 2 -0.07514989419751572 1103 3 -0.187119714500664 1103 4 -1.3453754358516221 1103 5 -3.0060817929865564 1103 6 -1.4337315242077224 1103 7 -3.24594294594295 1103 8 -2.676364993031669 1103 9 -3.2372882408596695 1103 10 -3.773554708078528 1103 11 -3.061747776033492 1103 12 -3.6977813632575476 1103 13 -2.953197552007076 1103 14 -2.2425395592062216 1103 15 -3.477621012144816 1103 16 -3.2401793092269173 1103 17 -3.7536065345589265 1103 18 -4.703162754353237 1103 19 -2.7905923441637626 1103 20 -1.2307039521325436 1103 21 -2.155967841682127 1103 22 -3.0247517033231333 1103 23 -1.9381290226528236 1103 24 -1.8117809879714528 1103 25 -2.178874476493526 1104 1 0.9930605549653251 1104 2 0.38318343913581643 1104 3 1.0212136188326717 1104 4 0.4046245641483779 1104 5 0.9939182070134436 1104 6 0.7746018091256133 1104 7 0.7957237207237142 1104 8 -0.05136499303166886 1104 9 0.4710450924736662 1104 10 0.8097786252548076 1104 11 0.8132522239665079 1104 12 0.7605519700757881 1104 13 -0.5365308853404116 1104 14 1.7574604407937784 1104 15 1.647378987855184 1104 16 2.176487357439747 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1.2165167724691486 2215 3 0.6601025077215574 2215 4 0.9879578974817136 2215 5 1.0772515403467793 2215 6 0.913490698014499 2215 7 0.7401681651681642 2215 8 0.03196834030166684 2215 9 0.026600648029216245 2215 10 0.782000847477029 2215 11 -0.15896999825571356 2215 12 1.1772186367424524 2215 13 1.1023580035484812 2215 14 1.4519048852382213 2215 15 1.7862678767440698 2215 16 2.56537624632864 2215 17 2.3158379098855164 2215 18 2.0329483567578777 2215 19 1.5010743225029017 2215 20 -1.1195928410214293 2215 21 1.1773654916512086 2215 22 1.391914963343531 2215 23 -1.285351244875045 2215 24 -1.5062254324158957 2215 25 2.071125523506474 2216 1 1.2013938882986608 2216 2 0.7998501058024843 2216 3 1.062880285499336 2216 4 1.4046245641483779 2216 5 1.2439182070134436 2216 6 1.6912684757922776 2216 7 1.62905705405705 2216 8 1.6986350069683311 2216 9 1.7627117591403305 2216 10 2.101445291921472 2216 11 1.8132522239665079 2216 12 2.1772186367424524 2216 13 -0.0781975520070759 2216 14 1.1324604407937784 2216 15 1.147378987855184 2216 16 2.0098206907730827 2216 17 1.8713934654410735 2216 18 1.0468372456467634 2216 19 2.0844076558362374 2216 20 2.2692960478674564 2216 21 2.344032158317873 2216 22 1.2252482966768667 2216 23 2.6868709773471764 2216 24 2.688219012028547 2216 25 2.946125523506474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 2.0 12 2.0 13 4.0 14 7.0 15 2.0 16 13.0 17 19.0 18 21.0 19 19.0 20 16.0 21 27.0 22 13.0 23 22.0 24 29.0 25 30.0 26 28.0 27 37.0 28 42.0 29 51.0 30 71.0 31 98.0 32 140.0 33 197.0 34 279.0 35 398.0 36 638.0 37 1153.0 38 2999.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02564102564102 21.393739185150228 12.82051282051282 24.760106968695926 2 15.002358861456203 21.811605598364523 39.55024374901714 23.635791791162134 3 17.990250039314358 25.271269067463436 30.397861298946378 26.34061959427583 4 12.97373800912093 17.10960842899827 37.411542695392356 32.50511086648844 5 13.22743000943693 37.76344762503932 35.215476564957534 13.793645800566216 6 31.294228652303822 37.75750904230225 17.770089636735335 13.178172668658593 7 26.136184934738164 32.11196729045447 23.55716307595534 18.194684698852022 8 23.730146249410282 36.15348325208366 21.84305708444724 18.273313414058816 9 23.793049221575718 17.172511401163703 22.141846202233058 36.89259317502752 10 14.420506368925931 30.460764271111813 33.275672275515014 21.84305708444724 11 31.703097971379147 25.161188866173926 24.13901556848561 18.996697593961315 12 20.474917439849033 27.582953294543167 32.03333857524768 19.90879069036012 13 27.31561566284007 23.52571158987262 26.969649315930177 22.189023431357132 14 20.333385752476804 22.45636106306023 29.108350369554962 28.101902814908 15 23.321276930334957 30.555118729359965 24.42207894323007 21.70152539707501 16 21.95313728573675 31.089793992766158 26.261990879069035 20.695077842428056 17 19.987419405566914 29.32851077213398 30.004717722912407 20.679352099386698 18 19.264035225664415 30.31923258373958 29.768831577292026 20.647900613303978 19 21.418462022330555 28.353514703569743 29.24988205692719 20.97814121717251 20 21.579112928593897 28.373702422145332 29.58477508650519 20.462409562755585 21 23.55716307595534 28.87246422393458 27.83456518320491 19.735807516905172 22 20.88378675892436 28.730932536562353 30.539392986318603 19.845887718194685 23 20.710803585469414 28.164805787073437 30.02044346595377 21.10394716150338 24 20.36483723855952 28.30633747444567 29.076898883472246 22.251926403522567 25 22.126120459191696 28.164805787073437 28.935367196100014 20.77370655763485 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 2.5 3 5.0 4 5.0 5 5.0 6 9.0 7 13.0 8 13.0 9 13.0 10 17.5 11 22.0 12 22.0 13 22.0 14 32.5 15 43.0 16 43.0 17 43.0 18 81.5 19 120.0 20 120.0 21 120.0 22 177.5 23 235.0 24 235.0 25 235.0 26 295.0 27 355.0 28 355.0 29 355.0 30 450.0 31 545.0 32 545.0 33 545.0 34 633.0 35 721.0 36 721.0 37 721.0 38 743.5 39 766.0 40 766.0 41 766.0 42 783.5 43 801.0 44 801.0 45 801.0 46 762.0 47 723.0 48 723.0 49 723.0 50 664.0 51 605.0 52 605.0 53 605.0 54 562.5 55 520.0 56 520.0 57 520.0 58 457.0 59 394.0 60 394.0 61 394.0 62 322.0 63 250.0 64 250.0 65 250.0 66 190.5 67 131.0 68 131.0 69 131.0 70 100.0 71 69.0 72 69.0 73 69.0 74 48.0 75 27.0 76 27.0 77 27.0 78 19.5 79 12.0 80 12.0 81 12.0 82 7.0 83 2.0 84 2.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03145148608271741 2 0.0 3 0.0 4 0.0 5 0.015725743041358705 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.015725743041358705 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6359.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.27944645384494 #Duplication Level Percentage of deduplicated Percentage of total 1 98.81991593921758 96.13146721182576 2 0.937601034594245 1.8241861927976097 3 0.04849660523763337 0.14153168737222832 4 0.0 0.0 5 0.032331070158422244 0.15725743041358703 6 0.032331070158422244 0.18870891649630445 7 0.032331070158422244 0.22016040257902186 8 0.0 0.0 9 0.016165535079211122 0.14153168737222832 >10 0.0808276753960556 1.1951564711432616 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTT 18 0.28306337474445664 No Hit TATCAACGCAGAGTACTTTTTTTTT 18 0.28306337474445664 No Hit GTATCAACGCAGAGTACTTTTTTTT 16 0.2516118886617393 No Hit ATTCCATTCCATTCCATTCCATTCC 12 0.18870891649630445 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12 0.18870891649630445 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 9 0.14153168737222832 No Hit GTGGTATCAACGCAGAGTACATGGG 7 0.11008020128951093 No Hit GAATGGAATGGAATGGAATGGAATG 7 0.11008020128951093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE