##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15393 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43091015396609 31.0 31.0 33.0 28.0 34.0 2 31.464236990839993 31.0 31.0 34.0 28.0 34.0 3 31.653608783213148 31.0 31.0 34.0 29.0 34.0 4 35.23127395569415 37.0 35.0 37.0 32.0 37.0 5 35.377379328266095 37.0 35.0 37.0 33.0 37.0 6 35.235041902163324 37.0 35.0 37.0 33.0 37.0 7 35.15656467225362 37.0 35.0 37.0 32.0 37.0 8 35.087702202299745 37.0 35.0 37.0 32.0 37.0 9 36.84129149613461 39.0 37.0 39.0 33.0 39.0 10 36.87559280192295 39.0 37.0 39.0 33.0 39.0 11 36.9465341388943 39.0 37.0 39.0 33.0 39.0 12 36.89495225102319 39.0 37.0 39.0 33.0 39.0 13 36.83115701942442 39.0 37.0 39.0 33.0 39.0 14 38.01351263561359 40.0 38.0 41.0 33.0 41.0 15 38.029104138244655 40.0 38.0 41.0 33.0 41.0 16 38.07730786721237 40.0 38.0 41.0 33.0 41.0 17 38.05983239134672 40.0 38.0 41.0 33.0 41.0 18 38.03800428766322 40.0 38.0 41.0 33.0 41.0 19 38.129604365620736 40.0 38.0 41.0 33.0 41.0 20 38.09790164360424 40.0 38.0 41.0 33.0 41.0 21 38.09978561683882 40.0 38.0 41.0 33.0 41.0 22 38.04411095952706 40.0 38.0 41.0 33.0 41.0 23 38.038134216851816 40.0 38.0 41.0 33.0 41.0 24 38.043656207366986 40.0 38.0 41.0 33.0 41.0 25 36.888390826999284 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3271863355028586 1101 2 -0.3227356346293817 1101 3 -0.8135631305096425 1101 4 -0.30799383105549794 1101 5 -0.4270051157310988 1101 6 -0.012413834030333248 1101 7 -1.235919371814859 1101 8 0.5587330861333442 1101 9 0.21108056712598255 1101 10 -0.920484060809784 1101 11 -0.22801822197801158 1101 12 -1.168193605412057 1101 13 -0.44088349134515425 1101 14 -1.2117559918795848 1101 15 0.5301679953226994 1101 16 0.44082121984720857 1101 17 -0.7505343237809683 1101 18 -0.22499938688529397 1101 19 -1.4924025568884716 1101 20 -0.5332761713088416 1101 21 0.332002096966292 1101 22 0.09451770755871536 1101 23 0.03673827010712216 1101 24 -0.23756600511867987 1101 25 -0.13011093266185725 1102 1 -0.41051966883619073 1102 2 0.4634962494285908 1102 3 -0.3443602319589196 1102 4 -0.8369793383018802 1102 5 -0.3853384490644345 1102 6 -1.9182109354796069 1102 7 -0.2920787921047179 1102 8 -1.1604698124173822 1102 9 -1.8794991430189398 1102 10 -0.6288173941431197 1102 11 0.18864844468865272 1102 12 -0.35659940251350264 1102 13 -0.46805740438863097 1102 14 -1.2642922237636398 1102 15 -1.971643598880199 1102 16 -0.8870773308774318 1102 17 -1.2161140339258907 1102 18 -1.9333327202186297 1102 19 -0.12102574529426846 1102 20 -0.8176964611639193 1102 21 -0.3926355841931297 1102 22 -1.467076495339839 1102 23 -0.7929718748204095 1102 24 -0.7266964399012892 1102 25 -0.4018500630966386 1103 1 0.2561469978304771 1103 2 0.7189310320372861 1103 3 0.936436869490354 1103 4 0.6920061689445021 1103 5 0.3229948842689012 1103 6 1.0709194993030025 1103 7 1.139080628185141 1103 8 1.6003997528000085 1103 9 1.127747233792654 1103 10 1.5378492725235446 1103 11 0.8969817780219884 1103 12 1.2901397279212787 1103 13 1.1841165086548457 1103 14 0.7882440081204152 1103 15 0.863501328656028 1103 16 1.1908212198472086 1103 17 1.8744656762190317 1103 18 1.6916672797813703 1103 19 1.8409307764448641 1103 20 1.7583904953578227 1103 21 2.0403354302996206 1103 22 1.8861843742253797 1103 23 2.036738270107122 1103 24 2.220767328214656 1103 25 1.7032224006714785 1104 1 0.4590455485551139 1104 2 -0.014764620136627826 1104 3 0.525204985432385 1104 4 -0.05437064264970104 1104 5 0.04944415963121429 1104 6 0.9513542819116978 1104 7 0.5774864252865868 1104 8 0.8830084484521805 1104 9 1.1205008569810602 1104 10 1.1537913015090524 1104 11 0.8843006186016993 1104 12 0.16513972792127873 1104 13 0.3145512912635411 1104 14 0.08353386319288347 1104 15 0.637052053293715 1104 16 0.7650965821660449 1104 17 0.4795381399871488 1104 18 1.2405803232596284 1104 19 1.5311481677492154 1104 20 -0.6872616785552239 1104 21 0.04214702450251906 1104 22 0.532923504660161 1104 23 1.1635498643100206 1104 24 0.9254774731421875 1104 25 0.685106458642494 1105 1 0.4394803311638107 1105 2 0.4939310320372847 1105 3 0.7947702028236883 1105 4 1.1086728356111664 1105 5 1.014661550935564 1105 6 0.6209194993029996 1105 7 0.40574729485180683 1105 8 0.6003997528000085 1105 9 -0.005586099540678902 1105 10 0.9211826058568775 1105 11 -0.9113515553113487 1105 12 -0.5848602720787213 1105 13 -0.16588349134515568 1105 14 -0.6534226585462477 1105 15 -0.4281653380106363 1105 16 0.5824878865138743 1105 17 0.05779900955236883 1105 18 -0.48333272021862683 1105 19 0.015930776444861294 1105 20 -0.2916095046421745 1105 21 -1.101331236367045 1105 22 0.41118437422537824 1105 23 -0.12992839655954214 1105 24 0.6124339948813216 1105 25 -1.1301109326618572 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1.3039164579424423 2208 17 1.8149418666952215 2208 18 1.9595244226385162 2208 19 1.6802164907305794 2208 20 2.4369619239292533 2208 21 1.4986687636329563 2208 22 1.582612945653949 2208 23 1.2629287462976038 2208 24 1.405291137738466 2208 25 1.6913176387667122 2209 1 0.3587111003945793 2209 2 -0.2906843525780971 2209 3 -0.22061441256092706 2209 4 0.8586728356111664 2209 5 0.4608153970894122 2209 6 0.7055348839183893 2209 7 1.1326703717748856 2209 8 0.9080920604923151 2209 9 1.179029285074705 2209 10 0.9481056827799605 2209 11 1.5732638293040395 2209 12 1.318985881767432 2209 13 0.837962662500999 2209 14 1.3811927260691377 2209 15 1.2641423542970571 2209 16 0.520949424975413 2209 17 0.4962605480139075 2209 18 0.8743595874736769 2209 19 0.8505461610602509 2209 20 1.4699289568962826 2209 21 0.8832841482483431 2209 22 0.8573382203792264 2209 23 1.8123792957481513 2209 24 1.397049379496707 2209 25 1.3506582981073763 2210 1 -0.03551966883619073 2210 2 -0.1977356346293817 2210 3 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0.863501328656028 2211 16 -0.22584544681946284 2211 17 0.8327990095523674 2211 18 0.15000061311470603 2211 19 0.6742641097781998 2211 20 0.25839049535782266 2211 21 0.707002096966292 2211 22 0.7195177075587154 2211 23 0.7867382701071222 2211 24 -0.6542326717853442 2211 25 0.24488906733814275 2212 1 0.15469772246815694 2212 2 0.5504527711677198 2212 3 -0.17044718848065799 2212 4 0.6847597921329083 2212 5 0.04944415963121429 2212 6 0.9513542819116978 2212 7 0.3166168600691961 2212 8 1.0134432310608759 2212 9 -0.09689044736676777 2212 10 0.5016173884655757 2212 11 0.8843006186016993 2212 12 0.8173136409647555 2212 13 -0.3376226217799356 2212 14 0.9530990805841881 2212 15 -0.14555664235846422 2212 16 0.33031397347039615 2212 17 0.044755531291500006 2212 18 0.6753629319552843 2212 19 -0.07754748442469861 2212 20 -0.2959573307291308 2212 21 0.3464948505894796 2212 22 -0.07577214751375294 2212 23 0.07659334257089512 2212 24 -1.0745225268578125 2212 25 -1.0540239761401153 2213 1 0.43563417731765597 2213 2 0.6323925704988262 2213 3 0.5101548182083029 2213 4 0.9740574509957796 2213 5 0.845430781704799 2213 6 0.7055348839183893 2213 7 0.863439602544112 2213 8 0.7927074451077019 2213 9 1.2559523619977782 2213 10 1.717336452010727 2213 11 1.265571521611733 2213 12 0.8959089586905122 2213 13 1.2225780471163858 2213 14 0.9965773414537509 2213 15 1.3410654312201302 2213 16 0.8286417326677196 2213 17 1.188568240321601 2213 18 1.2205134336275236 2213 19 1.7736230841371707 2213 20 0.8545443415116694 2213 21 0.5371303020944964 2213 22 0.8957997588407665 2213 23 0.9277639111327645 2213 24 0.35858784103516683 2213 25 1.0429659904150625 2214 1 0.7561469978304736 2214 2 0.727264365370619 2214 3 0.628103536157024 2214 4 0.5920061689445006 2214 5 0.7479948842688984 2214 6 0.9209194993030039 2214 7 1.522413961518474 2214 8 0.7670664194666728 2214 9 1.4610805671259826 2214 10 1.3045159391902104 2214 11 1.421981778021987 2214 12 0.5318063945879459 2214 13 1.1174498419881758 2214 14 1.2632440081204166 2214 15 1.6718346619893651 2214 16 1.0824878865138743 2214 17 1.5244656762190374 2214 18 1.1666672797813717 2214 19 1.0659307764448656 2214 20 1.2083904953578255 2214 21 1.265335430299622 2214 22 1.5111843742253797 2214 23 1.7867382701071222 2214 24 0.9457673282146501 2214 25 1.41988906733814 2215 1 1.0228136644971393 2215 2 0.06059769870395115 2215 3 0.3947702028236897 2215 4 0.25867283561116494 2215 5 0.4479948842689012 2215 6 0.9209194993030039 2215 7 -0.6442527051481974 2215 8 0.500399752800007 2215 9 -1.3389194328740146 2215 10 0.2711826058568789 2215 11 0.22198177802198416 2215 12 0.398473061254613 2215 13 0.3841165086548486 2215 14 1.1632440081204152 2215 15 1.4051679953226994 2215 16 1.1158212198472057 2215 17 1.0244656762190374 2215 18 1.0666672797813703 2215 19 1.2992641097781998 2215 20 0.14172382869115552 2215 21 0.8653354302996235 2215 22 0.8445177075587154 2215 23 1.1200716034404579 2215 24 0.9791006615479887 2215 25 0.6865557340048127 2216 1 0.9579013837953916 2216 2 0.9623520846688685 2216 3 0.6052965186131622 2216 4 1.4376202040322212 2216 5 0.9830826035671478 2216 6 1.031445815092475 2216 7 1.739957821167593 2216 8 1.1530313317473784 2216 9 1.0838875846698457 2216 10 1.7922352374358255 2216 11 1.1886484446886527 2216 12 1.3230344647633814 2216 13 1.1314849297074758 2216 14 1.7860510256642783 2216 15 1.0718346619893637 2216 16 1.403540518092818 2216 17 1.3262200621839497 2216 18 1.698246227149788 2216 19 0.7554044606553916 2216 20 1.324179969042035 2216 21 1.2618266583697988 2216 22 0.4848685847516947 2216 23 1.4358610771246703 2216 24 1.3282234685655325 2216 25 0.9619943304960401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 4.0 12 10.0 13 8.0 14 15.0 15 25.0 16 39.0 17 26.0 18 30.0 19 40.0 20 43.0 21 49.0 22 47.0 23 55.0 24 64.0 25 79.0 26 75.0 27 96.0 28 107.0 29 117.0 30 169.0 31 234.0 32 339.0 33 447.0 34 687.0 35 986.0 36 1641.0 37 2847.0 38 7112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40350877192983 21.124106562703055 12.696556205328136 24.775828460038987 2 15.468069901903464 21.756642629766777 39.46599103488599 23.309296433443773 3 18.24855453777691 25.881894367569675 29.83823816020269 26.031312934450725 4 12.772039238614955 18.274540375495356 37.75092574546872 31.20249464042097 5 14.087069525666015 36.523716699155294 35.2956465237167 14.09356725146199 6 31.36490612616124 38.12122393295654 17.137659975313454 13.376209965568764 7 26.551029688819593 31.910608718248557 23.263821217436497 18.274540375495356 8 23.062430975118563 37.42610277398818 21.03553563307997 18.47593061781329 9 23.764048593516534 17.47547586565322 22.35431689729098 36.40615864353927 10 15.045800038978758 30.176054050542454 33.008510361852785 21.769635548626 11 32.40433963489898 25.22575196517898 23.6860910803612 18.683817319560838 12 20.69525666016894 26.621182586094864 31.89083820662768 20.79272254710851 13 27.214243940476962 23.5752810449022 26.362986548833582 22.84748846578725 14 20.647089397089395 23.453742203742202 27.930093555093556 27.969074844074843 15 22.71162216591957 30.923146884947705 24.27726888845579 22.08796206067693 16 22.042486844669654 30.88416812837004 25.927369583576947 21.145975443383357 17 19.97011628662379 30.104592996816738 28.389527707399466 21.535763009160007 18 19.918144611186904 29.727798349899302 29.376989540700315 20.977067498213472 19 21.353862145130904 29.31852140583382 29.071655947508606 20.25596050152667 20 21.991943866943867 28.410862785862783 28.800675675675674 20.796517671517673 21 22.724615084778797 28.409017085688298 28.18813746508153 20.678230364451373 22 21.457805496004678 27.889300331319433 29.916195673358022 20.73669849931787 23 21.607224062885727 28.396024166829076 28.844279867472228 21.152471902812966 24 21.529266549730398 27.408562333528224 28.675371922302347 22.386799194439032 25 21.875 27.96257796257796 28.449844074844076 21.71257796257796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 5.0 2 7.5 3 10.0 4 10.0 5 10.0 6 17.5 7 25.0 8 25.0 9 25.0 10 37.0 11 49.0 12 49.0 13 49.0 14 81.5 15 114.0 16 114.0 17 114.0 18 192.5 19 271.0 20 271.0 21 271.0 22 410.5 23 550.0 24 550.0 25 550.0 26 723.5 27 897.0 28 897.0 29 897.0 30 1097.0 31 1297.0 32 1297.0 33 1297.0 34 1442.5 35 1588.0 36 1588.0 37 1588.0 38 1686.5 39 1785.0 40 1785.0 41 1785.0 42 1865.5 43 1946.0 44 1946.0 45 1946.0 46 1871.5 47 1797.0 48 1797.0 49 1797.0 50 1676.0 51 1555.0 52 1555.0 53 1555.0 54 1435.0 55 1315.0 56 1315.0 57 1315.0 58 1118.0 59 921.0 60 921.0 61 921.0 62 755.0 63 589.0 64 589.0 65 589.0 66 469.5 67 350.0 68 350.0 69 350.0 70 264.5 71 179.0 72 179.0 73 179.0 74 135.0 75 91.0 76 91.0 77 91.0 78 63.5 79 36.0 80 36.0 81 36.0 82 25.0 83 14.0 84 14.0 85 14.0 86 10.0 87 6.0 88 6.0 89 6.0 90 3.5 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019489378288832584 2 0.0 3 0.0 4 0.0 5 0.019489378288832584 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.019489378288832584 13 0.02598583771844345 14 0.006496459429610862 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.006496459429610862 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006496459429610862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 15393.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.84876242447866 #Duplication Level Percentage of deduplicated Percentage of total 1 97.41087162803308 93.3671149223673 2 2.2366815778771856 4.287663223543169 3 0.1762233970448692 0.5067238355096472 4 0.027111291853056795 0.1039433508737738 5 0.033889114816320995 0.16241148574027156 6 0.013555645926528397 0.07795751315533034 7 0.020333468889792598 0.1364256480218281 8 0.006777822963264199 0.0519716754368869 9 0.0 0.0 >10 0.0745560525959062 1.3057883453517831 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 32 0.2078867017475476 No Hit GGTATCAACGCAGAGTACTTTTTTT 31 0.2013902423179367 No Hit TATCAACGCAGAGTACTTTTTTTTT 26 0.16890794516988242 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 21 0.1364256480218281 No Hit GTACATAAGCAGTGGTATCAACGCA 18 0.11693626973299552 No Hit GTGGTATCAACGCAGAGTACATGGG 17 0.11043981030338465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE