FastQCFastQC Report
Wed 25 May 2016
SRR1295211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7026285
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN80050.11392933819223104No Hit
GTGGTATCAACGCAGAGTACATGGG71270.10143340328495072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.010.6796591
GTGGTAT21050.09.4371651
TGGTATC19750.09.2392122
TGTCGCC4200.08.82036310
GTTCTAG8150.08.6302131
GTCTTAG6400.08.6138121
CTGTCGC4900.08.5298879
CTAATAC7300.08.4626593
GTCGTAG2553.5106495E-108.2003021
TGAACCG1401.0345652E-48.14664655
TACACTG14150.08.1274555
TTACACT9600.08.118364
GTATTAG9550.08.0617441
GTATCAA71700.08.020171
GTCTAGG5700.08.0041241
GTCTAGA6750.07.8855431
CCGTGCG1100.00686316247.77206669
TTAGACT5750.07.76883034
CGTTATA1356.2519725E-47.74390032
TCGACCG1356.28504E-47.7400839
Produced by FastQC (version 0.11.5)