FastQCFastQC Report
Wed 25 May 2016
SRR1295211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7026285
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT184400.26244309759709433No Hit
GGTATCAACGCAGAGTACTTTTTTT149760.21314250702896337No Hit
TATCAACGCAGAGTACTTTTTTTTT104250.1483714366838237No Hit
GTGGTATCAACGCAGAGTACATGGG82700.11770089029978145No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71110.10120568693128731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA500.001501451613.29883919
AGCGTCA5550.012.3244773
GGTATCA55500.011.745331
GTGGTAT24650.010.3312281
TGGTATC23400.010.0682862
ACGGACC900.00111412529.5003888
CGTGTCG1304.2424235E-69.5003879
CAGCGTC7600.08.999922
AGACCGT2651.8189894E-128.9618026
GACCGTG3950.08.8992247
TAGACAG9850.08.7768825
TGCCGCG1107.146304E-48.636847
GCAGCGT6850.08.6007281
GTATCAA101900.08.5419171
GTCTTAG6800.08.5242271
CTAGGAC7350.08.4014643
GCGTCAG8150.08.3927424
CGTCAGA8500.08.2708085
CTAGACT6800.08.2427494
TAGACCG1050.0045148478.1430735
Produced by FastQC (version 0.11.5)