FastQCFastQC Report
Wed 25 May 2016
SRR1295210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG51890.11935315146142632No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51310.11801908270352254No Hit
GTATCAACGCAGAGTACTTTTTTTT45790.1053224283179555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11600.011.554761
TGGTATC11450.011.1238192
GGTATCA27150.011.0991361
TACACCG1606.1318133E-910.0988875
TAATACG1002.742065E-49.5052754
CGTATAC800.0044984189.5050543
TCTAGAC2500.09.5050543
GTTCTAG4200.09.2797081
TAAGACT4850.09.2112964
ATCGTAC850.0074068468.9459343
GTGTTAG4800.08.9119151
ACACCGT1502.7109654E-68.8651376
TAGACAG4350.08.7400785
GTATCAA49200.08.655911
GTACTAG2659.094947E-128.6092451
TAGCGTG1000.00289321578.5543525
GTCTAAG3450.08.5416621
GTGTAAG5850.08.4498171
GGACCGT1951.5423211E-78.2806226
GTATAAA8950.08.072171
Produced by FastQC (version 0.11.5)