FastQCFastQC Report
Wed 25 May 2016
SRR1295210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137990.3173933584536947No Hit
GGTATCAACGCAGAGTACTTTTTTT118950.2735991012976809No Hit
TATCAACGCAGAGTACTTTTTTTTT80620.18543555734862574No Hit
GTGGTATCAACGCAGAGTACATGGG60800.13984720772508616No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51690.11889312775180433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.012.3111391
TGGTATC15700.011.3765812
GTGGTAT16650.011.357621
TCGCACT600.005876022411.08397111
GTACCGT1158.8271736E-710.7392546
AGCGTCA4000.010.6882383
GTGCTAC3450.09.9158951
TAGGACG1252.5706995E-69.8806824
CGAATTA900.00111623839.49825215
CTAGAAC4300.09.2797113
TAGACTG4750.09.0009335
TACGACC950.00182368479.0006224
CGTTCCG950.00182387569.00051810
ACCGTTC950.00182387569.0005188
GTAGGAC3400.08.9417933
ATAGGAC3650.08.8499253
GTCGTAG1201.7596134E-48.7108381
TGTACCG1752.624529E-78.6866145
ACGCTGC1107.144748E-48.6368618
TGTGCGG1651.050339E-68.6368610
Produced by FastQC (version 0.11.5)