FastQCFastQC Report
Wed 25 May 2016
SRR1295209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809628
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN75350.11065215309852464No Hit
GTGGTATCAACGCAGAGTACATGGG68190.10013762866341598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.010.1715791
TTACGCT1752.557499E-99.7763024
CCGGCGT850.0074488558.939913
GTCTTAC6300.08.9021821
GTGGTAT21550.08.8661231
GTCCTAT6050.08.7986821
AGCCTCG5350.08.7048882
GTCTAGG6500.08.6282681
GTCCTAA6250.08.5171241
TGGTATC20800.08.3619722
CTAGGAC6000.08.2371963
CTTACGC1502.573872E-58.2371963
TATACCG1856.081482E-78.2203135
TAGACAG8750.08.0382925
GTATCAA72050.08.0082881
CCTATAC5500.07.94918253
GTATTAA10000.07.79468971
ACACCGT1951.3608551E-67.7939896
GTATTAT11300.07.739171
TTATACT11300.07.7383714
Produced by FastQC (version 0.11.5)