FastQCFastQC Report
Wed 25 May 2016
SRR1295209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809628
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT158580.232876157111666No Hit
GGTATCAACGCAGAGTACTTTTTTT128280.1883803344323655No Hit
TATCAACGCAGAGTACTTTTTTTTT94420.13865661971549692No Hit
GTGGTATCAACGCAGAGTACATGGG78520.11530732662635904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52350.011.7072321
AGCGTCA5300.010.5756633
GTGGTAT22000.010.4089181
TGGTATC22050.09.9094762
CCTATAC5450.09.0643843
CTAGTAC5050.09.0298673
GCAGCGT6000.08.7100911
CTAGAAC7650.08.6929683
GCGTCAG6450.08.690084
AGTACCG1901.01234036E-78.5004035
CAGCGTC6600.08.49257852
GTATCAA94500.08.3958741
TACACCG2159.729774E-98.3957485
GTCTTAG7150.08.3723181
TAGACTG8750.08.3603985
GTATTAG9150.08.2038421
GTCTAGA6900.08.1248271
TCTAGAC6800.08.1030883
GTCCTAG7650.08.07352351
CTTACAC9100.08.0386083
Produced by FastQC (version 0.11.5)