FastQCFastQC Report
Wed 25 May 2016
SRR1295208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5211026
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG60430.11596564668838727No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN60230.11558184511073252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG550.003059213312.096655
TTTAGCG1103.7964128E-812.0965333
CGGTCGT752.0774073E-411.39893110
GGTATCA36250.010.3833591
TAGCGTC856.5697497E-410.06365
CGTAGCG1151.0219594E-59.9176553
GTGGTAT16600.09.9057811
TAGAGCG900.00110960959.5045115
TGGTATC16350.09.3590922
GTCTTAG5100.09.3185971
TACGTCG1151.10588895E-49.091275
ACGTCGC1151.11275775E-49.0861036
TATCCCG1054.4746735E-49.0519155
ATTAGAC4450.08.9704633
CTATGCG850.0074465528.94016559
AGGACCG1502.6877588E-68.8708775
CTAGGAC4550.08.773313
GTATTAG7350.08.6643931
GCGTCAG2201.5934347E-98.6408821
ACCGTTC1451.6927253E-58.5165258
Produced by FastQC (version 0.11.5)