FastQCFastQC Report
Wed 25 May 2016
SRR1295208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5211026
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137130.2631535517189897No Hit
GGTATCAACGCAGAGTACTTTTTTT116230.22304628685406672No Hit
TATCAACGCAGAGTACTTTTTTTTT80550.15457608540045664No Hit
GTGGTATCAACGCAGAGTACATGGG68770.13197017247659099No Hit
ATTCCATTCCATTCCATTCCATTCC55390.10629384693148719No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCG400.005280316314.2504049
GGTATCA44250.011.6825661
AGCGTCA4150.011.4465473
CGGATAG752.0741309E-411.4008695
TGGTATC17200.010.8263032
TAGACAG7650.010.4321685
GTGGTAT19050.010.02661
CCGCGTG850.00743793238.9414299
CGTAGTA1606.743812E-78.9068442
CTGATCG1401.0859256E-58.8216789
ATACCGT1304.373508E-58.7691426
TCCGTAC2201.6061676E-98.636943
GTACGAT1107.150155E-48.6362766
GTGTTAG6300.08.5977921
ACCGACC1000.00290603178.5502418
TTAGACA5400.08.4447644
GCAGCGT5300.08.4270311
TATACTG8800.08.4210975
GTCCTAG4800.08.3149691
CAGCGTC5750.08.261422
Produced by FastQC (version 0.11.5)