FastQCFastQC Report
Wed 25 May 2016
SRR1295207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5144069
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG60760.11811661157733304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59290.11525895161981692No Hit
GTATCAACGCAGAGTACTTTTTTTT54050.10507246306377305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14800.011.2408091
GGTATCA32850.011.0836971
TGGTATC14050.010.8238082
GTTGCGC750.002652090710.132079
TAGACAG6750.09.7167255
GTATTAG7150.09.4400341
TGAACCG1151.1046387E-49.092215
GTCTTAC4250.08.7236241
TCAGGAC5650.08.5796763
GTCTTAG5150.08.4912541
GTTCTAG6800.08.3880991
TATACTG9350.08.3363675
GTCCTGG9000.08.2389771
GTATCAA57600.08.21917251
GCGCATT1050.0045209788.14176213
TTATACT8650.08.1317874
TTAGACA6500.08.043034
TACACCG1909.09562E-78.0046245
CTAGTAC2854.9112714E-118.0041563
GTGTTAG6300.07.9975431
Produced by FastQC (version 0.11.5)