FastQCFastQC Report
Wed 25 May 2016
SRR1295207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5144069
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152270.2960108038986258No Hit
GGTATCAACGCAGAGTACTTTTTTT132180.2569561178125721No Hit
TATCAACGCAGAGTACTTTTTTTTT88660.17235383117916964No Hit
GTGGTATCAACGCAGAGTACATGGG69950.13598184627772295No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59930.11650310289383756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCG500.001500028113.3004027
AGCGTCA4950.012.8583493
GTGGTAT17750.012.3665191
GGTATCA46150.012.1894421
CAGCGTC5500.011.5725152
GCAGCGT5200.011.5125371
TGGTATC18400.010.997082
TTACGCA750.002649755410.1331464
ACGGATC951.6445144E-410.0005948
CGTCAGA6550.09.7177555
GCGTCAG6600.09.6437614
AACGTTA900.00111382929.5005648
CTAGTAC3050.09.344093
ATCGTTC950.0018239649.0005348
GTGTTAG6350.08.9786571
GCGGTAT850.0074241598.9434461
GTCGTAC850.0074241598.9434461
GACCGTC1107.1472535E-48.6366247
GTATCAA84450.08.6191211
GAACCGC1000.00290750348.549766
Produced by FastQC (version 0.11.5)