FastQCFastQC Report
Wed 25 May 2016
SRR1295206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608724
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63340.13743500370167536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN60150.13051334816317922No Hit
GTATCAACGCAGAGTACTTTTTTTT59920.13001429462905567No Hit
GGTATCAACGCAGAGTACTTTTTTT50430.10942291185152334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15000.011.1532162
GTGGTAT15550.011.0659431
GGTATCA33950.010.949071
ATAGGAC3800.09.7561543
GCTAGAC2303.6379788E-129.5059973
TAGGACC2850.09.006274
GTCTTAT5300.08.96880151
GTCTAGA5000.08.7463741
CTAGAAC4700.08.6969763
ACCGTTC1000.00290953768.5490598
GTCTAAT4600.08.4735681
CCTACAC4500.08.4497743
GTCCTAC2951.8189894E-128.3789891
GTCTTAG4550.08.3577391
GTATCAA60550.08.3529091
GCACCGT1951.5422302E-78.2806886
GTCCTAA4400.08.2105291
GTACTAA4400.08.2105291
TATACTG7700.08.1482635
GCGGGAC1050.0044897378.1479973
Produced by FastQC (version 0.11.5)