##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295206_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4608724
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.961949337821054	31.0	30.0	33.0	25.0	34.0
2	29.995915355313098	31.0	30.0	33.0	25.0	34.0
3	29.907734548651643	31.0	30.0	34.0	25.0	34.0
4	32.918566831079495	35.0	33.0	37.0	25.0	37.0
5	33.44879233384338	35.0	33.0	37.0	27.0	37.0
6	33.658807947709604	35.0	35.0	37.0	28.0	37.0
7	33.54864621964778	36.0	35.0	37.0	27.0	37.0
8	33.54309327267157	36.0	35.0	37.0	27.0	37.0
9	34.44386407170401	38.0	34.0	39.0	25.0	39.0
10	34.43721667862948	37.0	34.0	39.0	25.0	39.0
11	34.987842621949156	38.0	35.0	39.0	27.0	39.0
12	34.96961176238803	38.0	35.0	39.0	27.0	39.0
13	34.940301046450166	38.0	35.0	39.0	27.0	39.0
14	35.52938904564474	39.0	34.0	41.0	25.0	41.0
15	35.92755543616845	39.0	35.0	41.0	27.0	41.0
16	35.938771772837775	39.0	35.0	41.0	27.0	41.0
17	35.6050937309329	39.0	34.0	41.0	25.0	41.0
18	35.49634649417062	39.0	34.0	41.0	25.0	41.0
19	35.913356495203445	39.0	35.0	41.0	27.0	41.0
20	35.91301041242652	39.0	35.0	41.0	27.0	41.0
21	35.975020200819145	39.0	35.0	41.0	27.0	41.0
22	35.914946740138916	39.0	35.0	41.0	26.0	41.0
23	35.644441064381375	39.0	34.0	41.0	25.0	41.0
24	35.909245378981254	39.0	35.0	41.0	27.0	41.0
25	34.55967899140847	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1299261687853459
1101	2	0.16461171978997768
1101	3	-0.11138131947300423
1101	4	-0.29667110693660703
1101	5	-0.299012745615471
1101	6	-0.3342323931477651
1101	7	-0.32944714486067994
1101	8	-0.21858279431997119
1101	9	-0.164916848456798
1101	10	-0.060229716798374966
1101	11	0.11470148042624828
1101	12	0.028440093576442393
1101	13	0.10931211076448477
1101	14	0.4531946491680614
1101	15	0.12885256815135193
1101	16	0.08123517540928304
1101	17	0.11678942674907233
1101	18	0.36469574963354745
1101	19	0.1754801242452686
1101	20	0.15061499156254143
1101	21	0.2018810655634553
1101	22	0.15230854998745968
1101	23	0.1750911434802518
1101	24	0.05329917481974178
1101	25	0.14612017409455547
1102	1	-0.24695830969783117
1102	2	0.13823113569998569
1102	3	-0.23959580506129896
1102	4	-0.3979447302643706
1102	5	-0.6919418205823291
1102	6	-0.5332184663233761
1102	7	-0.6131226075160541
1102	8	-0.5730654928922121
1102	9	-0.47742291340490084
1102	10	-0.0637539800609801
1102	11	-0.07202958037340323
1102	12	-0.22664648138884047
1102	13	-0.32157076270539875
1102	14	0.01640942183582439
1102	15	-0.07040115142059022
1102	16	-0.15188925240347828
1102	17	-0.32712029275220544
1102	18	0.15894034260217893
1102	19	-0.08989941689448244
1102	20	-0.041379655509530266
1102	21	-0.0920664159293807
1102	22	-0.1471470085335369
1102	23	-0.0807174205734853
1102	24	-0.1349097458434798
1102	25	-0.1505681907323435
1103	1	-0.4623452008799305
1103	2	-0.14918054051942065
1103	3	-0.589642370762693
1103	4	-0.7836304544912451
1103	5	-0.7689334887595152
1103	6	-0.870517822320771
1103	7	-0.8155730676518615
1103	8	-0.72300717638295
1103	9	-0.3853474582173604
1103	10	-0.27788649333793103
1103	11	-0.3324701939989225
1103	12	-0.4581567727213667
1103	13	-0.2896944878781724
1103	14	-0.4377784285788948
1103	15	-0.3651385396160265
1103	16	-0.45946523158538355
1103	17	-0.3036876817602021
1103	18	-0.06675025695987102
1103	19	-0.3235553140707452
1103	20	-0.29497238167611783
1103	21	-0.3400311301701535
1103	22	-0.263981999272481
1103	23	-2.5697180810355604
1103	24	-1.2084555521176625
1103	25	-0.6244001416789686
1104	1	-0.3254539318201104
1104	2	-0.0813565043295057
1104	3	-0.3315272074060083
1104	4	-0.8208630266467338
1104	5	-0.48453008127286523
1104	6	-0.5872659867976111
1104	7	-0.50764075330639
1104	8	-0.5161696767808053
1104	9	-2.784585342635097
1104	10	-0.9941006389045981
1104	11	-0.641697862527721
1104	12	-0.4718936011550099
1104	13	-0.38532664605914846
1104	14	-0.15829951078548277
1104	15	-0.31343055592448366
1104	16	-0.20872513920089375
1104	17	0.12163394419862783
1104	18	0.2706094645108479
1104	19	-0.13919673328873472
1104	20	-0.24589077469111942
1104	21	-0.18094127860342724
1104	22	-0.18607341248414144
1104	23	-0.1585065587448966
1104	24	-0.2022822393050916
1104	25	-0.14200966473556065
1105	1	-0.20416748828025177
1105	2	-0.13879389244296192
1105	3	-0.2667913817217631
1105	4	-0.09408368880524165
1105	5	-0.15362719929102298
1105	6	-0.30172365037099524
1105	7	-0.24676717190080666
1105	8	-0.41522703068169164
1105	9	-2.215004299664301
1105	10	-0.6313175318954052
1105	11	-0.36649316067794047
1105	12	-0.2735934894790759
1105	13	-0.2401030355200504
1105	14	-0.047551537935333954
1105	15	-0.012200994658449815
1105	16	-0.19703553569211607
1105	17	-1.425450772220266
1105	18	-0.31576567437776504
1105	19	-0.19577326944393292
1105	20	-0.15920386895795957
1105	21	-0.14344830863509372
1105	22	-0.11225312043321622
1105	23	-0.03779625484710181
1105	24	-0.1346173591517399
1105	25	-0.6305325360073724
1106	1	-0.3188491810960272
1106	2	-0.3923472708567033
1106	3	-0.5070537696221855
1106	4	-0.5854375014908584
1106	5	-0.42383622252966546
1106	6	-0.4782437326233193
1106	7	-0.5480487845348421
1106	8	-0.6398635600845566
1106	9	-3.4322292352589123
1106	10	-1.0228041977286324
1106	11	-0.8376902214575423
1106	12	-0.48277076292144727
1106	13	-0.60209298674976
1106	14	-0.8776590488484075
1106	15	-0.7881382710223335
1106	16	-0.8117187754704887
1106	17	-0.6400243206151117
1106	18	-0.25728148391939953
1106	19	-0.4261560500816728
1106	20	-0.5310659806924889
1106	21	-0.3710546861580255
1106	22	-0.39446766161275804
1106	23	-0.23333296690464067
1106	24	-0.44446705349219684
1106	25	-0.7833073398829242
1107	1	-0.21864492066021057
1107	2	0.05490128403326722
1107	3	-0.1108723411636845
1107	4	-1.3998867860474533
1107	5	-0.38637129683611704
1107	6	-0.22848991074128122
1107	7	-0.06983543334499842
1107	8	-0.06039942780991936
1107	9	0.23543094741818038
1107	10	0.4313890652534198
1107	11	0.21281806368099865
1107	12	-0.360197261169084
1107	13	0.0024003393426639263
1107	14	0.2553681047493157
1107	15	-0.5713289411788693
1107	16	-0.9071410637926149
1107	17	-0.06071810181379789
1107	18	-0.03999107737382701
1107	19	0.0391966150887626
1107	20	-0.2568434569490705
1107	21	-0.028995578833168167
1107	22	-0.004494378518280939
1107	23	0.1976240896175625
1107	24	-0.09093528473108137
1107	25	-0.30484803983393505
1108	1	0.22838481438583358
1108	2	0.23733927852305925
1108	3	0.26670686994101445
1108	4	0.711001094180773
1108	5	0.4452521110157761
1108	6	0.4238489273601118
1108	7	0.40435180592701414
1108	8	0.3651394711606315
1108	9	0.6825027237309484
1108	10	0.6954092094500197
1108	11	0.4367687590023337
1108	12	0.3550245955414226
1108	13	0.2851173064889849
1108	14	0.33658474273724437
1108	15	0.4528808932131625
1108	16	0.5325265035463715
1108	17	0.5962495621871184
1108	18	0.7035593389253947
1108	19	0.35726913347109956
1108	20	0.3790034989911888
1108	21	0.3620770653432359
1108	22	0.42207461392072076
1108	23	0.5034856808646708
1108	24	0.31800951409651645
1108	25	0.3803739932850192
1109	1	0.2781035677732895
1109	2	0.10439940019977101
1109	3	0.27487461814316205
1109	4	0.6621271818483407
1109	5	0.5692951759929343
1109	6	0.4376221143806944
1109	7	0.42271908879616404
1109	8	0.41644370833819266
1109	9	0.8326978976847883
1109	10	0.686703722528037
1109	11	0.35338807870631683
1109	12	0.44151485526018064
1109	13	0.2679662091783612
1109	14	0.6968612845398567
1109	15	0.481370006265756
1109	16	0.47902640120585005
1109	17	0.799001453278926
1109	18	0.5835169509411458
1109	19	0.3483952060608715
1109	20	0.3692248501477664
1109	21	0.3537663473532646
1109	22	0.2903225425954332
1109	23	0.5473286182944648
1109	24	0.377102723774712
1109	25	0.38922449626673483
1110	1	0.4317586071032302
1110	2	0.29878666356281514
1110	3	0.42995396224113236
1110	4	0.6976633465617255
1110	5	0.6123481677755933
1110	6	0.610861161943788
1110	7	0.6057491977106224
1110	8	0.6116867178987349
1110	9	1.2188277011913513
1110	10	1.1159542723958467
1110	11	0.7460350579807624
1110	12	0.7005919987662779
1110	13	0.6041649617292251
1110	14	0.8013716458229609
1110	15	0.6112985982051882
1110	16	0.6262657988016542
1110	17	1.03589303686352
1110	18	1.1638539852326986
1110	19	0.6611114028008842
1110	20	0.656001858409752
1110	21	0.5734707520394622
1110	22	0.6391526590271184
1110	23	0.8030579136185665
1110	24	0.5683049883038507
1110	25	0.6167546170369107
1111	1	0.25803697379188506
1111	2	0.2275932189455041
1111	3	0.27337336623219954
1111	4	0.7088918774651134
1111	5	0.6073166025801555
1111	6	0.4935721830789461
1111	7	0.3234151450229561
1111	8	0.31198528394241976
1111	9	0.744825228326718
1111	10	0.6893783936049118
1111	11	0.33368857664977725
1111	12	0.12385400963019322
1111	13	0.2341713865769961
1111	14	0.22348886308837024
1111	15	0.24431574736989603
1111	16	0.1395936044482866
1111	17	0.44481623611855525
1111	18	0.5145691718658867
1111	19	0.38155965054640006
1111	20	-0.24528618208019992
1111	21	-0.30537020675217974
1111	22	-0.11183489542805347
1111	23	0.152319272856559
1111	24	0.2804115607610882
1111	25	0.1346869930453849
1112	1	0.1583032178609045
1112	2	0.132740527756809
1112	3	0.2458398996025295
1112	4	0.7640850737436438
1112	5	0.6093743751981222
1112	6	0.48356351335839776
1112	7	0.4125019780483328
1112	8	0.5124303351522315
1112	9	-1.1722068741266796
1112	10	0.19183615216304872
1112	11	0.19166776262044039
1112	12	0.31211310651511326
1112	13	0.19786222348858473
1112	14	0.5563664932044503
1112	15	0.3265524927585375
1112	16	0.24376877473798686
1112	17	0.29805405441126
1112	18	0.4088853583071028
1112	19	0.20770239421408832
1112	20	0.1445565098426087
1112	21	0.2278413971270794
1112	22	0.2921579272547774
1112	23	-0.03915073086773191
1112	24	0.09739364605377432
1112	25	0.16617685398890103
1113	1	0.33200184992120185
1113	2	0.14962607748669754
1113	3	0.3012822869290268
1113	4	0.6039204767693747
1113	5	0.7067762658195349
1113	6	0.5146956369769882
1113	7	0.46417815476277013
1113	8	0.49871631558916363
1113	9	0.287622137983476
1113	10	0.649627229800906
1113	11	0.41907445804628196
1113	12	0.1456101127859455
1113	13	0.29348124278872945
1113	14	-0.011835668023181256
1113	15	0.12249570988070957
1113	16	0.27066472095265226
1113	17	0.734462524650155
1113	18	0.4937222659549434
1113	19	0.4193214089660131
1113	20	0.4396509803526527
1113	21	0.4499881360224194
1113	22	0.39512368154142763
1113	23	0.059395186703824265
1113	24	0.14084921881696744
1113	25	0.22982209730090375
1114	1	0.335690153588974
1114	2	0.22908050569321503
1114	3	0.38387673305880554
1114	4	0.7620044819757652
1114	5	0.6429254301089458
1114	6	0.6419382682633881
1114	7	0.33005242533965884
1114	8	0.35851988484773756
1114	9	0.09883497655220452
1114	10	0.3951991988816346
1114	11	0.3319749377613519
1114	12	0.3446843225021752
1114	13	0.40243060522973906
1114	14	0.8005757346944051
1114	15	0.44255485489248514
1114	16	0.30713019418542586
1114	17	0.7089882441487774
1114	18	0.5174615331094756
1114	19	0.40842245349897155
1114	20	0.22952992337376088
1114	21	0.3012664326445176
1114	22	0.04439849958460229
1114	23	0.6004353388385653
1114	24	0.4003084323701458
1114	25	0.30090654250815163
1115	1	0.33256916458103447
1115	2	0.22894379533776998
1115	3	0.47799166701287277
1115	4	0.898379642791518
1115	5	0.729483000571193
1115	6	0.6042108374818156
1115	7	0.6263367781699003
1115	8	0.614959161691985
1115	9	0.7568003830930863
1115	10	0.5535492943990192
1115	11	0.4448709411916525
1115	12	0.511001013275056
1115	13	0.4127153574811757
1115	14	0.33234260378920055
1115	15	0.3403787680958459
1115	16	0.4135166852336525
1115	17	0.7095046891406085
1115	18	0.8472741455774866
1115	19	0.6078178997376895
1115	20	0.547458512790719
1115	21	0.4126061028895265
1115	22	-0.237237406516293
1115	23	0.26594095671688933
1115	24	0.43501000401437295
1115	25	0.5126179877434467
1116	1	0.49814171726717404
1116	2	0.379417516165109
1116	3	0.6453966684324008
1116	4	1.101530813910344
1116	5	0.9348529883790917
1116	6	0.7222098646781276
1116	7	0.7053149453715335
1116	8	0.7580008573067829
1116	9	1.105362742340077
1116	10	0.7381132508311765
1116	11	0.43482982069136966
1116	12	0.5717883335633758
1116	13	0.4158651571964356
1116	14	0.8667786991596742
1116	15	0.5010371782344762
1116	16	0.6138978354273235
1116	17	0.9458058630067114
1116	18	0.9565351713720034
1116	19	0.6215719168296303
1116	20	0.6021107663630261
1116	21	0.6684907768493105
1116	22	0.7481480027961496
1116	23	0.970282418634163
1116	24	0.7081650416698437
1116	25	0.4602909774912618
1201	1	-0.27879241848777525
1201	2	0.10304029651675606
1201	3	-0.29930514250950324
1201	4	-0.4917703031535581
1201	5	-0.6044881510968665
1201	6	-0.6145260622587188
1201	7	-0.5140358879674096
1201	8	-0.5800753830136287
1201	9	2.151907468430636E-4
1201	10	0.11235772159130164
1201	11	-0.05190784335565013
1201	12	-0.021350774481987855
1201	13	-0.08559740064180943
1201	14	0.49868850961215117
1201	15	0.12137371195329649
1201	16	0.028270763817339173
1201	17	0.16951254810392413
1201	18	0.2726333806084682
1201	19	0.003790582237087392
1201	20	-0.041510299312328414
1201	21	-0.19391188763314915
1201	22	-0.06395882444699907
1201	23	-0.08760437784707875
1201	24	-0.16999522304351444
1201	25	-0.04710550423310167
1202	1	-0.37336945706644187
1202	2	-0.3377147079921379
1202	3	-0.615970898246534
1202	4	-0.7082035115677954
1202	5	-0.8924558872285075
1202	6	-0.781703731066365
1202	7	-0.8265753633340651
1202	8	-0.6928409158541555
1202	9	-0.14797570299602825
1202	10	-0.09087374462173159
1202	11	-0.3243495814295443
1202	12	-0.32905661372565476
1202	13	-0.19197402143539932
1202	14	-0.2873916082025332
1202	15	-0.3312370837466787
1202	16	-0.2734827169623415
1202	17	0.060417977739895434
1202	18	0.1817519038043045
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2211	13	-0.11780302526071296
2211	14	0.2114997307733475
2211	15	0.06848999312143889
2211	16	0.05541125254364232
2211	17	0.3389135218382293
2211	18	0.2941248898375548
2211	19	0.06093433659351888
2211	20	0.2186014965293097
2211	21	-0.06353246656523481
2211	22	0.030403605495465058
2211	23	-2.05200319488668
2211	24	-0.7781703858221647
2211	25	-0.42361047921472306
2212	1	0.06772859885021631
2212	2	-1.6488800246678643
2212	3	-0.23408608686042953
2212	4	0.5242219241980592
2212	5	0.434425136413239
2212	6	0.41998785058964927
2212	7	0.4210270463344017
2212	8	0.35249327974417355
2212	9	0.822020023651362
2212	10	0.7182572386013462
2212	11	0.4559092163679992
2212	12	0.2833149246360094
2212	13	0.2641093574282678
2212	14	0.5401573275997649
2212	15	0.2799959040818649
2212	16	0.38648295747157135
2212	17	0.6800620655945337
2212	18	0.8126659003193168
2212	19	0.4760538186686176
2212	20	0.4184821485493586
2212	21	0.3682504632339416
2212	22	0.3406950426930351
2212	23	0.5975677895845735
2212	24	0.36252824398896166
2212	25	0.18046557850114908
2213	1	0.18867235895345758
2213	2	0.0349114275549276
2213	3	0.21499013147955992
2213	4	0.6189217838139172
2213	5	0.44456912803019577
2213	6	0.3809698255793137
2213	7	0.27149192903350894
2213	8	0.38985047043794907
2213	9	0.623706353063632
2213	10	0.5958613970425688
2213	11	0.2799496495287528
2213	12	0.24948764496016906
2213	13	0.09345534521136045
2213	14	-1.2887683468421898
2213	15	-0.39405298224571084
2213	16	-0.046820918194548256
2213	17	0.40915904627776456
2213	18	0.2275934144536862
2213	19	0.06122415644082935
2213	20	0.1705740879929678
2213	21	0.2150186527691602
2213	22	0.30699085159091055
2213	23	0.5475713038941663
2213	24	0.21456762671731155
2213	25	-0.08899462769358735
2214	1	0.4228830129454053
2214	2	0.36595571083985234
2214	3	0.5530193557119851
2214	4	1.0636357685663498
2214	5	0.6845482663769147
2214	6	0.572585208767876
2214	7	0.43767438843732265
2214	8	0.396242324916912
2214	9	0.5314834734337168
2214	10	0.43766229285833447
2214	11	0.38879027510327546
2214	12	0.19143004431149535
2214	13	0.11685789183967188
2214	14	0.39883833206664576
2214	15	0.1773824706028222
2214	16	0.20626657936225712
2214	17	0.19264469378080662
2214	18	0.4879371282670846
2214	19	0.213993637758783
2214	20	0.3565415063125883
2214	21	0.3383660799896475
2214	22	0.410588398463382
2214	23	0.5282202529819102
2214	24	0.399119504953795
2214	25	0.3457820909133318
2215	1	0.3306707112455669
2215	2	0.1955513918898255
2215	3	0.4022242014542279
2215	4	0.9472329454460748
2215	5	0.6077782830972325
2215	6	0.6393313177577511
2215	7	0.49622755883260083
2215	8	0.43693282024729285
2215	9	0.702362612456902
2215	10	0.7551348901093249
2215	11	0.47295420483506945
2215	12	0.33009329204676163
2215	13	0.2420139595165196
2215	14	0.40109795759497047
2215	15	0.3108397127858282
2215	16	0.34869290773040973
2215	17	0.5868339598537275
2215	18	0.6930356730470706
2215	19	0.3527495871102957
2215	20	-0.5351136214921226
2215	21	-0.020867082913873958
2215	22	-0.7701733252346301
2215	23	0.15025670015686643
2215	24	0.06952549391934326
2215	25	0.12759390146475624
2216	1	0.03784001163834105
2216	2	-0.5946993022142344
2216	3	0.1610463436529379
2216	4	0.6000224696287617
2216	5	0.37353026624574426
2216	6	0.37275105959553656
2216	7	0.27589753878820744
2216	8	0.24597529746589686
2216	9	0.6135281256305873
2216	10	0.3052057463051625
2216	11	0.14767016757019746
2216	12	-0.22147755223276988
2216	13	-0.056088747718476384
2216	14	-0.09374041478079675
2216	15	-0.033077062391456025
2216	16	0.0774446237203108
2216	17	0.12643834845467694
2216	18	0.4445647519198559
2216	19	0.16507739855465076
2216	20	-1.0327329766415758
2216	21	-1.2597676427469864
2216	22	-1.298543036081682
2216	23	-1.783090710529578
2216	24	-1.5593789007980803
2216	25	-1.4448035145465283
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10204.0
3	1361.0
4	1082.0
5	842.0
6	973.0
7	1269.0
8	946.0
9	1937.0
10	3693.0
11	6721.0
12	11119.0
13	16812.0
14	23077.0
15	28630.0
16	32168.0
17	34490.0
18	35319.0
19	35670.0
20	35784.0
21	36272.0
22	36901.0
23	38105.0
24	40157.0
25	43235.0
26	47257.0
27	53149.0
28	61863.0
29	74315.0
30	91732.0
31	114232.0
32	148193.0
33	195691.0
34	265265.0
35	367076.0
36	526400.0
37	819974.0
38	1366810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77955229417775	21.13089849997533	13.672373087750541	23.417176118096382
2	15.853269967902317	21.535199938015865	39.75653379833044	22.854996295751388
3	17.838607112524226	26.68307236655851	30.242339772207583	25.235980748709686
4	13.511434617410522	18.054587984752974	37.86293707877994	30.571040319056564
5	13.220223925517633	38.40566844008752	35.03621965728193	13.337887977112914
6	30.783891223183886	38.816612244142455	17.454180009167807	12.945316523505848
7	25.720138127147617	33.491673891186	23.363257107304637	17.424930874361742
8	24.111506575475357	36.7718694806421	21.200367570586252	17.91625637329629
9	25.098620381763343	17.131417620349097	21.769482888160546	36.00047910972702
10	15.007429679461778	30.3156628012582	33.96939638674243	20.707511132537597
11	31.85872490920101	25.723573172690696	24.035754538208277	18.38194737990002
12	20.890201614949223	27.817575502981185	31.219426255087413	20.072796626982186
13	26.534906346966764	24.478255824509606	26.67889583098848	22.307941997535156
14	21.007094965766235	24.017349636411854	27.831966058429288	27.143589339392626
15	22.666731721087302	31.279770021006186	24.267310066503626	21.786188191402882
16	21.95755412191249	31.215306396576324	25.71080931748982	21.116330164021367
17	20.71929944549309	30.27817262332867	27.88833453202779	21.114193399150444
18	20.71539698490274	30.013794064523996	28.8158552265569	20.45495372401637
19	21.309568271922593	29.28529506881146	28.98670374649246	20.418432912773486
20	22.402649210185057	28.811924058122578	28.381026851944775	20.404399879747594
21	22.828652747993203	28.950977281304873	27.536010631196824	20.6843593395051
22	21.933990603297556	28.550407144001504	28.67916338699229	20.836438865708654
23	22.28500427990235	28.838597950565546	27.9505590207566	20.9258387487755
24	21.99763205289431	28.280007350302895	28.38954341033153	21.33281718647126
25	22.244065988493922	28.534526394853287	27.962880111468518	21.258527505184265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14134.0
1	14134.0
2	12239.5
3	10345.0
4	10345.0
5	10345.0
6	10900.5
7	11456.0
8	11456.0
9	11456.0
10	15738.5
11	20021.0
12	20021.0
13	20021.0
14	31134.0
15	42247.0
16	42247.0
17	42247.0
18	67093.5
19	91940.0
20	91940.0
21	91940.0
22	132852.0
23	173764.0
24	173764.0
25	173764.0
26	225916.5
27	278069.0
28	278069.0
29	278069.0
30	332727.5
31	387386.0
32	387386.0
33	387386.0
34	432559.0
35	477732.0
36	477732.0
37	477732.0
38	506012.5
39	534293.0
40	534293.0
41	534293.0
42	537478.0
43	540663.0
44	540663.0
45	540663.0
46	525592.5
47	510522.0
48	510522.0
49	510522.0
50	490113.5
51	469705.0
52	469705.0
53	469705.0
54	428316.0
55	386927.0
56	386927.0
57	386927.0
58	334592.0
59	282257.0
60	282257.0
61	282257.0
62	234158.0
63	186059.0
64	186059.0
65	186059.0
66	144677.0
67	103295.0
68	103295.0
69	103295.0
70	77072.5
71	50850.0
72	50850.0
73	50850.0
74	36820.5
75	22791.0
76	22791.0
77	22791.0
78	15971.5
79	9152.0
80	9152.0
81	9152.0
82	6325.5
83	3499.0
84	3499.0
85	3499.0
86	2360.0
87	1221.0
88	1221.0
89	1221.0
90	770.0
91	319.0
92	319.0
93	319.0
94	191.0
95	63.0
96	63.0
97	63.0
98	38.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16345088141533318
2	0.30377171642302725
3	0.3650468112215008
4	0.3946862515524905
5	0.4022154505238326
6	0.3595138263866528
7	0.3628553152673061
8	0.3796495515895506
9	0.36613171020872587
10	0.3981796262913553
11	0.3148376860927233
12	0.36350625465964115
13	0.3326951234224484
14	0.34478089814013596
15	0.3839674495587065
16	0.33193569413139085
17	0.3822750071386353
18	0.350834634488852
19	0.36515530112022326
20	0.3266196890939878
21	0.36788924656803057
22	0.3205225567857828
23	0.3036198305648158
24	0.3410054496645926
25	0.3362969880600357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4608724.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.56111157960237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.96708692721438	69.19198106769082
2	9.70832207390962	15.448097915460798
3	1.860959649934218	4.441800550606622
4	0.5920496797010331	1.8841652250944692
5	0.26705601548398283	1.0623636722962586
6	0.14346213774303998	0.6848404289053373
7	0.0994731382379832	0.5539935415367774
8	0.06255767806695176	0.3981726723876473
9	0.04825459588546966	0.34552703587252254
>10	0.23170368317632892	3.1853388276230583
>50	0.012504039851621162	0.6661592815883786
>100	0.005667547778830964	0.8192982842304457
>500	4.1037888167664595E-4	0.24485466190352032
>1k	3.830198827062751E-4	0.5644125243665746
>5k	1.0943425219926826E-4	0.5089943104366009
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6334	0.13743500370167536	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6015	0.13051334816317922	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5992	0.13001429462905567	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5043	0.10942291185152334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1697979744501947E-5
2	0.0	0.0	0.0	0.0	2.1697979744501947E-5
3	0.0	0.0	0.0	0.0	2.1697979744501947E-5
4	0.0	0.0	0.0	0.0	2.1697979744501947E-5
5	0.0	0.0	0.0	2.1697979744501947E-5	4.3395959489003895E-5
6	0.0	0.0	0.0	2.1697979744501947E-5	4.3395959489003895E-5
7	0.0	0.0	0.0	2.1697979744501947E-5	4.3395959489003895E-5
8	0.0	0.0	0.0	2.1697979744501947E-5	4.3395959489003895E-5
9	0.0	0.0	0.0	8.679191897800779E-5	4.3395959489003895E-5
10	0.0	0.0	0.0	8.679191897800779E-5	4.3395959489003895E-5
11	0.0	0.0	0.0	8.679191897800779E-5	4.3395959489003895E-5
12	0.0	0.0	0.0	8.679191897800779E-5	4.3395959489003895E-5
13	0.0	0.0	0.0	8.679191897800779E-5	4.3395959489003895E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTATC	1500	0.0	11.153216	2
GTGGTAT	1555	0.0	11.065943	1
GGTATCA	3395	0.0	10.94907	1
ATAGGAC	380	0.0	9.756154	3
GCTAGAC	230	3.6379788E-12	9.505997	3
TAGGACC	285	0.0	9.00627	4
GTCTTAT	530	0.0	8.9688015	1
GTCTAGA	500	0.0	8.746374	1
CTAGAAC	470	0.0	8.696976	3
ACCGTTC	100	0.0029095376	8.549059	8
GTCTAAT	460	0.0	8.473568	1
CCTACAC	450	0.0	8.449774	3
GTCCTAC	295	1.8189894E-12	8.378989	1
GTCTTAG	455	0.0	8.357739	1
GTATCAA	6055	0.0	8.352909	1
GCACCGT	195	1.5422302E-7	8.280688	6
GTCCTAA	440	0.0	8.210529	1
GTACTAA	440	0.0	8.210529	1
TATACTG	770	0.0	8.148263	5
GCGGGAC	105	0.004489737	8.147997	3
>>END_MODULE