FastQCFastQC Report
Wed 25 May 2016
SRR1295206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608724
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT173840.37719767987842184No Hit
GGTATCAACGCAGAGTACTTTTTTT153560.33319417695657194No Hit
TATCAACGCAGAGTACTTTTTTTTT102230.22181844692804342No Hit
GTGGTATCAACGCAGAGTACATGGG72150.15655092385658156No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65600.1423387471239328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.012.4972841
GTGGTAT19900.012.3684121
TGGTATC19650.011.9910552
AGCGTCA4350.011.5761543
CAGCGTC4650.010.829072
GCAGCGT4800.010.097091
GTGTTAG5950.09.4232841
TAGACAG5500.08.6376275
TCTATAC5800.08.5183023
TACCCCG1252.741009E-48.3612225
GTGCTAG5050.08.2799651
GTGTAGA8000.08.196461
CCTATAC3850.08.1438713
CGAGACT2451.3951649E-98.143874
GTCTAGA4450.08.1150431
GTCCTAT3550.08.0308251
GTCTAGG4900.07.9516091
GTAGGAC3001.8189894E-117.91765263
TCCGTAC1200.00168981077.91765263
TTAGACA5200.07.85674764
Produced by FastQC (version 0.11.5)