FastQCFastQC Report
Wed 25 May 2016
SRR1295205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127230
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA58584.604260001571956No Hit
GTACTGGTTCACTATCGGTCAGTCA11320.8897272655820169No Hit
ATACAGGGTGACAGCCCCGTACACA10210.8024836909533916No Hit
GTATCAACGCAGAGTACTTTTTTTT8810.6924467499803505No Hit
GGATACCACGTGTCCCGCCCTACTC8370.6578637113888234No Hit
GGTATCAACGCAGAGTACTTTTTTT6360.4998821032775289No Hit
TATCAACGCAGAGTACTTTTTTTTT5870.4613691739369646No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5360.4212842882967854No Hit
GTACATAAGCAGTGGTATCAACGCA5290.4157824412481333No Hit
GTGGTATCAACGCAGAGTACATGGG5110.4016348345515995No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5050.3969189656527548No Hit
CCCATATTCAGACAGGATACCACGT5020.3945610312033326No Hit
GCCTTGGAGGATGGTCCCCCCATAT4810.37805549005737643No Hit
GTTCACTATCGGTCAGTCAGGAGTA4700.3694097304094946No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4560.35840603631219053No Hit
GTATTTAGCCTTGGAGGATGGTCCC4460.35054625481411616No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3800.29867169692682544No Hit
CACACACACTGATTCAGGCTCTGGG3730.29316984987817335No Hit
CATCTAAGTACCCCGAGGAAAAGAA3520.27666430873221726No Hit
CTATCGGTCAGTCAGGAGTATTTAG3360.2640886583352983No Hit
TATTCAGACAGGATACCACGTGTCC3350.26330268018549086No Hit
GGTTAATGAGGCGAACCGGGGGAAC3310.2601587675862611No Hit
ATCATTAACTGAATCCATAGGTTAA3270.25701485498703136No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3080.24208127014069009No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3050.23972333569126777No Hit
GTCCCGCCCTACTCATCGAGCTCAC3050.23972333569126777No Hit
CGGTACTGGTTCACTATCGGTCAGT3030.2381513793916529No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2980.23422148864261574No Hit
GCCCTACTCATCGAGCTCACAGCAT2980.23422148864261574No Hit
TCACGGTACTGGTTCACTATCGGTC2970.23343551049280828No Hit
GCTTATGTACTCTGCGTTGATACCA2930.23029159789357856No Hit
TTCCAGACGCTTCCACTAACACACA2920.22950561974377112No Hit
AGCTCACAGCATGTGCATTTTTGTG2860.2247897508449265No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2800.22007388194608188No Hit
GGGTGACAGCCCCGTACACAAAAAT2650.20828420969897038No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2610.2051402970997406No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2520.1980664937514737No Hit
CTACTGGGGGAATCTCGGTTGATTT2490.19570855930205142No Hit
GGTTCACTATCGGTCAGTCAGGAGT2420.19020671225339936No Hit
GTCCCCCCATATTCAGACAGGATAC2340.18391888705493986No Hit
GTTAATGATAGTGTGTCGAAACACA2330.18313290890513242No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2310.18156095260551758No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2260.1776310618564804No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2250.17684508370667296No Hit
TTTGTGTACGGGGCTGTCACCCTGT2210.1737011711074432No Hit
GGAGTATTTAGCCTTGGAGGATGGT2140.16819932405879118No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2110.16584138960936887No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2070.16269747701013912No Hit
CACTGATTCAGGCTCTGGGCTGCTC1930.15169378291283503No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1930.15169378291283503No Hit
TAAGTACCCCGAGGAAAAGAAATCA1900.14933584846341272No Hit
CTTATGTACTCTGCGTTGATACCAC1900.14933584846341272No Hit
GGTATCAACGCAGAGTACATGGGGA1880.14776389216379784No Hit
GTATCGCGCGCCTTTCCAGACGCTT1840.1446199795645681No Hit
AACACACACACTGATTCAGGCTCTG1780.13990411066572347No Hit
ACTTAGATGTTTCAGTTCCCCCGGT1770.13911813251591607No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1720.13518824176687888No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1670.1312583510178417No Hit
GTACGGGGCTGTCACCCTGTATCGC1650.12968639471822682No Hit
CTAACACACACACTGATTCAGGCTC1630.12811443841861198No Hit
CTACTCATCGAGCTCACAGCATGTG1620.12732846026880454No Hit
GTACATGGGTAGGTTAATGAGGCGA1590.12497052581938223No Hit
GTTATAACCGGCGATTTCCGAATGG1580.12418454766957479No Hit
GGTTAGAAGAGAACGAAAAACAATA1560.1226125913699599No Hit
GTACACAAAAATGCACATGCTGTGA1560.1226125913699599No Hit
CCTATATATTTTTCTTTCTCTTCTT1560.1226125913699599No Hit
GTGCATTTTTGTGTACGGGGCTGTC1550.12182661322015248No Hit
GTACATGGGAGAGCTGATGACGAGT1530.1202546569205376No Hit
GGCTCTGGGCTGCTCCCCGTTCGCT1530.1202546569205376No Hit
GCATGTGCATTTTTGTGTACGGGGC1490.11711074432130787No Hit
GTACATGGGATATCAATAAGCGGAG1490.11711074432130787No Hit
GTACATGGGCATCTAAGTACCCCGA1470.11553878802169301No Hit
GTACTTAGATGTTTCAGTTCCCCCG1460.11475280987188556No Hit
GGGCTGTCACCCTGTATCGCGCGCC1460.11475280987188556No Hit
GGAGGATGGTCCCCCCATATTCAGA1450.11396683172207812No Hit
CAGCATGTGCATTTTTGTGTACGGG1450.11396683172207812No Hit
TCATTAACCTATGGATTCAGTTAAT1400.11003694097304094No Hit
GGTATCAACGCAGAGTACATGGGGG1360.1068930283738112No Hit
GTACATGGGAGTGTACCTGACGTTT1350.10610705022400377No Hit
AAGTACCCCGAGGAAAAGAAATCAA1350.10610705022400377No Hit
CCGCTACTGGGGGAATCTCGGTTGA1350.10610705022400377No Hit
TTTCCAGACGCTTCCACTAACACAC1340.10532107207419635No Hit
CCACGTGTCCCGCCCTACTCATCGA1320.10374911577458147No Hit
GGGCTGCTCCCCGTTCGCTCGCCGC1300.10217715947496661No Hit
AAAATGCACATGCTGTGAGCTCGAT1300.10217715947496661No Hit
GAGTACATAAGCAGTGGTATCAACG1300.10217715947496661No Hit
CTGTGAGCTCGATGAGTAGGGCGGG1300.10217715947496661No Hit
GGGTTTCCCCATTCGGAAATCGCCG1300.10217715947496661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAAAA402.711893E-416.6443521
CTGACAC350.00214439816.2982353
CGAATGG350.002155746816.28537619
CTGAACC350.002161440216.2789549
AATAACT400.005217090714.2609564
TAGTGTG400.005258269614.2440859
GTCACAG400.005258269614.24408515
ACTGAAC400.005258269614.2440858
ACCGACT400.005258269614.24408513
GTGTGTC400.005258269614.24408511
AAATAAC500.00147774413.31022553
ACAGGGT1950.012.6764053
TACAGGG2250.012.2538592
TGTGACT550.003042273212.09065819
GAACCGA550.003051285412.08589111
ATAGGTT951.0152435E-611.99975117
CCCCGTA2450.011.62782515
ACGCTTC907.344408E-611.6062927
CAGGGTG2150.011.4972054
AGGGTGA2250.011.4087655
Produced by FastQC (version 0.11.5)