##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295205_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127230
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.838685844533522	31.0	28.0	33.0	16.0	34.0
2	28.68849327988682	31.0	28.0	33.0	16.0	34.0
3	29.03004794466714	31.0	28.0	33.0	18.0	34.0
4	31.805784799182582	35.0	32.0	37.0	17.0	37.0
5	32.55016898530221	35.0	32.0	37.0	20.0	37.0
6	32.50591841546805	35.0	32.0	37.0	19.0	37.0
7	32.63779768922424	35.0	32.0	37.0	21.0	37.0
8	32.75799732767429	35.0	32.0	37.0	22.0	37.0
9	33.33852864890356	37.0	32.0	39.0	17.0	39.0
10	33.49450601273285	37.0	32.0	39.0	17.0	39.0
11	33.89338992376012	38.0	33.0	39.0	19.0	39.0
12	33.989279258036625	38.0	33.0	39.0	21.0	39.0
13	33.85774581466635	38.0	33.0	39.0	19.0	39.0
14	34.22500196494538	38.0	32.0	41.0	17.0	41.0
15	34.58063349838874	38.0	32.0	41.0	17.0	41.0
16	34.53090466085043	38.0	32.0	41.0	18.0	41.0
17	34.244470643716106	38.0	32.0	40.0	17.0	41.0
18	34.075532500196495	38.0	32.0	40.0	17.0	41.0
19	34.3881710288454	38.0	32.0	40.0	18.0	41.0
20	34.42837381120805	38.0	32.0	40.0	18.0	41.0
21	34.4203804134245	38.0	32.0	40.0	18.0	41.0
22	34.36927611412403	38.0	32.0	40.0	18.0	41.0
23	34.076397076161285	38.0	32.0	40.0	18.0	41.0
24	34.40135188241767	38.0	32.0	40.0	21.0	41.0
25	33.164741020199635	37.0	30.0	40.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6816876157809588
1101	2	0.42617820044815247
1101	3	0.6011484774162774
1101	4	0.4534304536484477
1101	5	1.0746796506729623
1101	6	0.7081389926687578
1101	7	0.7143200579477167
1101	8	0.5540590630155791
1101	9	1.446937706232184
1101	10	0.7604143730952302
1101	11	0.3302480916047159
1101	12	1.0295589485272814
1101	13	0.5576097972024101
1101	14	1.1562180550203394
1101	15	1.0397853774637795
1101	16	0.49811731709939266
1101	17	0.8924537206758174
1101	18	0.978185886510424
1101	19	0.6614579943970327
1101	20	0.5850692123064363
1101	21	0.6155939579755412
1101	22	0.8727025640737125
1101	23	0.7947030334835432
1101	24	0.5752558640265377
1101	25	1.1251536889263818
1102	1	-0.48326913799158433
1102	2	-0.8182549979324136
1102	3	-0.483089652874483
1102	4	-0.8299473527556778
1102	5	-0.6028139083995434
1102	6	-0.3310586517920484
1102	7	-0.9187080981067517
1102	8	-1.0694227183609364
1102	9	-0.3219480133114345
1102	10	-0.35543030853892077
1102	11	-0.47942250096061656
1102	12	-0.2482290529449287
1102	13	-0.23363514206884162
1102	14	-0.3145677381338743
1102	15	-0.800318597508614
1102	16	-0.7697792600077094
1102	17	-1.2753554438438783
1102	18	0.04061687657299018
1102	19	-0.4343047954447101
1102	20	-0.4575789290259138
1102	21	-0.45587733389048424
1102	22	-0.3769063796141836
1102	23	-0.2667618174549915
1102	24	-0.1035488838571581
1102	25	-0.14872374942473954
1103	1	-1.2918584637433845
1103	2	-2.046833691042
1103	3	-1.9804661639809709
1103	4	-2.3860576548614
1103	5	-1.2944210553842659
1103	6	-1.923782226208985
1103	7	-2.0475017555045305
1103	8	-1.7246477374303524
1103	9	-1.8443805769339292
1103	10	-1.2868728807137586
1103	11	-1.3546738704911618
1103	12	-2.4194377069911113
1103	13	-2.1749013510696074
1103	14	-2.740428173147624
1103	15	-2.6144091598829284
1103	16	-3.0333301002175865
1103	17	-1.7110626301231449
1103	18	-1.9061043401711117
1103	19	-1.966491652574355
1103	20	-2.01921358219375
1103	21	-1.7953359937152769
1103	22	-1.996049758482961
1103	23	-3.5055570928635404
1103	24	-3.0195741248251906
1103	25	-1.7190956607577483
1104	1	-0.4968380252446849
1104	2	-0.2962576969877446
1104	3	-1.0405181892503883
1104	4	-0.8904798027618099
1104	5	-0.9983972724039596
1104	6	-0.991861007331245
1104	7	-0.8244619933343316
1104	8	-0.31773580877928964
1104	9	-2.445049473254997
1104	10	-1.276765114084256
1104	11	-0.31654678019015847
1104	12	-0.7563384873701509
1104	13	-0.8433517412591272
1104	14	-1.3149357911335073
1104	15	-0.7932274430490409
1104	16	-1.1589339649518848
1104	17	-1.092482176760079
1104	18	0.04677563010016428
1104	19	-0.8452727748337381
1104	20	-0.32839232615510383
1104	21	-0.7578034779218967
1104	22	-0.6853102564391094
1104	23	-0.4873482485677414
1104	24	-1.0590390077683338
1104	25	-0.8588206700479759
1105	1	0.8047108488930235
1105	2	0.7406549673360878
1105	3	0.34563305375364806
1105	4	0.664017138537158
1105	5	0.6866476319302848
1105	6	1.0082170871630964
1105	7	0.8378313816494014
1105	8	0.7489048263892641
1105	9	-1.5445939220380254
1105	10	0.8686728658714884
1105	11	0.2512262251463042
1105	12	0.9211052195151765
1105	13	1.2077220580380228
1105	14	1.223916219799726
1105	15	0.31084648640560175
1105	16	0.5077327017147795
1105	17	0.05891701626347867
1105	18	1.234384636998513
1105	19	1.2206389783876546
1105	20	0.9507954911037828
1105	21	0.9065203539146296
1105	22	0.6587480541166642
1105	23	1.7150466492586318
1105	24	1.8116625411058038
1105	25	0.1487284644047122
1106	1	0.12551217357610156
1106	2	-0.243247961625066
1106	3	0.15580705413367824
1106	4	-0.6121032773264616
1106	5	0.4892209916848884
1106	6	-0.08309918092236046
1106	7	-3.292633192515382E-4
1106	8	-0.3365209452114897
1106	9	-3.0576643093992466
1106	10	-0.8414984199940321
1106	11	-1.0887667171159734
1106	12	-0.14578041798855423
1106	13	0.07511802426946446
1106	14	0.3341797991585551
1106	15	-0.4510363008578935
1106	16	0.299176551982157
1106	17	-0.6288595249021327
1106	18	0.015567622421571059
1106	19	-0.09628059054743687
1106	20	0.28658093587698374
1106	21	-0.0812643307945109
1106	22	0.3621873439996648
1106	23	0.6704743210195332
1106	24	-0.027556764521385446
1106	25	-0.029669429132027858
1107	1	-0.3231200765267346
1107	2	-0.2997833380133841
1107	3	-0.09115921489141243
1107	4	-1.017723392505399
1107	5	-0.31955111855780416
1107	6	-0.676476391946629
1107	7	-0.2904876343599767
1107	8	-0.13824862929211434
1107	9	-0.03863921684474292
1107	10	-0.05208562690476981
1107	11	-0.030328831472210993
1107	12	-1.3550564360881054
1107	13	-0.2500825104898965
1107	14	0.026410362712653068
1107	15	-0.27752231484390677
1107	16	-0.598036529054454
1107	17	0.0703383360604306
1107	18	-0.05546795964342266
1107	19	0.11338107132010578
1107	20	0.19083844307566267
1107	21	-0.312290657409072
1107	22	-0.035951282080134206
1107	23	0.6745107257912366
1107	24	0.17140971018038442
1107	25	-0.3508078495351512
1108	1	0.5028958886401718
1108	2	0.415111437022901
1108	3	1.037832076835727
1108	4	1.0812425000925678
1108	5	1.3841861818776096
1108	6	0.8082841305497439
1108	7	0.7086052538838601
1108	8	1.0152346798515737
1108	9	1.2262374159564189
1108	10	1.680951383254886
1108	11	1.4015470756395487
1108	12	1.4435647540425194
1108	13	1.1921344706421806
1108	14	1.6299118140914572
1108	15	1.46277158936509
1108	16	1.7152798715261
1108	17	1.6410930530415655
1108	18	1.5639986586439463
1108	19	1.6140160495494271
1108	20	1.0253812587505564
1108	21	1.173376976843464
1108	22	1.0063201257573127
1108	23	1.90137937600894
1108	24	1.18619563180593
1108	25	1.427221903730448
1109	1	0.6161034358099862
1109	2	0.7169982294757311
1109	3	0.5236811334394993
1109	4	0.8359594812246449
1109	5	0.9455069365945903
1109	6	-0.02662152982761512
1109	7	0.5906807255819686
1109	8	0.34070637796477854
1109	9	0.6271808121828357
1109	10	0.034724968160546155
1109	11	0.5147546228093631
1109	12	0.655828904985917
1109	13	0.8336439046044433
1109	14	1.1393457763556114
1109	15	0.8684319667235769
1109	16	1.0926383620921385
1109	17	0.7448666379472257
1109	18	0.18192318694583776
1109	19	0.854582087285273
1109	20	0.907456730448672
1109	21	1.253565656088746
1109	22	0.8270748427384405
1109	23	0.741002017518376
1109	24	0.5588371412398914
1109	25	0.4555237905229035
1110	1	0.4179902282628163
1110	2	0.4764321917398817
1110	3	1.0802849070244065
1110	4	0.595393443488792
1110	5	1.2190918422549615
1110	6	0.728095451304462
1110	7	0.868982612374424
1110	8	0.27466864211572783
1110	9	1.5281242084092526
1110	10	0.7517061002360137
1110	11	1.4534338680923753
1110	12	1.1369609804576157
1110	13	0.5930778668685974
1110	14	1.2855721914499512
1110	15	0.22692253276131424
1110	16	0.8567893054883626
1110	17	1.1976968266264691
1110	18	1.464942054870363
1110	19	1.444204728794709
1110	20	0.8980227681845179
1110	21	1.2158298070321436
1110	22	1.1666974842478766
1110	23	1.5098699420466772
1110	24	1.4644975185983853
1110	25	1.9885426584474288
1111	1	0.01822607731941872
1111	2	0.36367820044815247
1111	3	0.40403309280089417
1111	4	0.8043919921099878
1111	5	0.8727565737498821
1111	6	0.6841005311302979
1111	7	-0.06452609589843661
1111	8	0.17905906301557906
1111	9	0.2690530908475637
1111	10	0.7988759115567703
1111	11	0.3302480916047159
1111	12	0.31321279468112806
1111	13	0.45664825874087
1111	14	0.612948824251113
1111	15	1.1022853774637795
1111	16	1.0173480863301663
1111	17	0.5414921822142773
1111	18	0.853185886510424
1111	19	0.8633810713201058
1111	20	0.12833844307566267
1111	21	-0.2882521958706121
1111	22	0.2188564102275592
1111	23	0.4389338027143097
1111	24	0.24352509479577122
1111	25	-0.0719616956889979
1112	1	0.32534330219980134
1112	2	0.6793526575699786
1112	3	0.4977715911446552
1112	4	1.2226842261916921
1112	5	0.5168033164808392
1112	6	0.24310402763379102
1112	7	0.2918182176827173
1112	8	0.7344050328352338
1112	9	-0.7525700228808105
1112	10	0.8550960072505518
1112	11	-0.07750862536252612
1112	12	0.44076616236607435
1112	13	0.4410060062565151
1112	14	0.19260929535894888
1112	15	0.458054608233013
1112	16	0.7948140414276992
1112	17	0.6528742212278971
1112	18	0.7726742928409323
1112	19	0.2738291758471547
1112	20	0.5232823149932244
1112	21	0.2618260153918115
1112	22	0.9436677830652442
1112	23	-0.15671397056504333
1112	24	0.26008747977920876
1112	25	0.3680226620585145
1113	1	-0.14112076952742925
1113	2	0.024324423594375588
1113	3	0.11700086826866851
1113	4	0.6328743205923111
1113	5	-0.1430958171025054
1113	6	0.5924078769376422
1113	7	0.4069309380586006
1113	8	-0.16804765909114394
1113	9	-0.43486236306788584
1113	10	-0.24391688123602506
1113	11	-0.3278426914860688
1113	12	-0.6812865123181808
1113	13	-0.42519512310251173
1113	14	-0.3551298313275453
1113	15	-0.19960304942464546
1113	16	0.656381350363425
1113	17	0.5005185162406107
1113	18	-0.009773226448693606
1113	19	0.7506385210775548
1113	20	0.49593199816921896
1113	21	0.4618777417593236
1113	22	0.3756045044756533
1113	23	-0.44784720906670117
1113	24	-0.08772923645856423
1113	25	-0.16027597150327466
1114	1	0.3606556547842068
1114	2	0.35372424595194474
1114	3	0.5071163358489237
1114	4	0.4950745489897237
1114	5	0.821248809213948
1114	6	0.9477561829901688
1114	7	0.8527499965537118
1114	8	0.8238315440556647
1114	9	0.3554561244337009
1114	10	0.4925378833877545
1114	11	0.8743524614133094
1114	12	0.8310215704124388
1114	13	0.47380246603592724
1114	14	1.1328793045689167
1114	15	0.33129404485273284
1114	16	0.14623035429693942
1114	17	0.4024979604735748
1114	18	0.012178302545088115
1114	19	0.22919454188709665
1114	20	0.7795888945021758
1114	21	0.5841972443605172
1114	22	0.5823676056049507
1114	23	0.8828214877991769
1114	24	0.9257208333295353
1114	25	1.1148160218985765
1115	1	0.1396883414703609
1115	2	0.27831898419271184
1115	3	0.5708509447602559
1115	4	1.1614311793378498
1115	5	0.7473937290474169
1115	6	0.8790388475308788
1115	7	0.8312467633178215
1115	8	0.18504600060629173
1115	9	0.7545393027488743
1115	10	0.4734042134435654
1115	11	0.18928292469615116
1115	12	0.2548855087595001
1115	13	0.31477598007614205
1115	14	-0.20971082741797176
1115	15	-0.07496425969151232
1115	16	0.3615062866204397
1115	17	0.8061873926642065
1115	18	0.8139986586439463
1115	19	0.10458208728527296
1115	20	0.412173711580742
1115	21	0.1969618825038424
1115	22	-0.960661006318162
1115	23	-0.3863564730476625
1115	24	0.24279940539083356
1115	25	-0.1388158321186097
1116	1	0.8313945265172791
1116	2	0.6818857282145068
1116	3	0.9312081236523966
1116	4	1.2488592895184603
1116	5	0.9116808847330162
1116	6	0.9222484126605295
1116	7	0.35261191315133544
1116	8	0.626045899708302
1116	9	0.8407314125258907
1116	10	0.8964694944444673
1116	11	0.7240675074829568
1116	12	0.40502172104887535
1116	13	0.37458326491117333
1116	14	0.8903295317788746
1116	15	0.8216909718693799
1116	16	0.887411846099809
1116	17	1.1144559831192566
1116	18	1.250861740068629
1116	19	0.9304573773669986
1116	20	0.8266878877486405
1116	21	0.9615164178685873
1116	22	1.2391926915932459
1116	23	1.1891908985596444
1116	24	0.8120154279919873
1116	25	0.20562468851503013
1201	1	-0.48581621123779684
1201	2	-0.11309657949826857
1201	3	-0.15884960907434476
1201	4	-0.5187997415295094
1201	5	-1.1533149915007854
1201	6	-0.35979059018620063
1201	7	-0.717917791267741
1201	8	-0.3755237919404095
1201	9	-0.18550106146778234
1201	10	-0.09291513321934985
1201	11	-0.07996048091729335
1201	12	-0.25201395617995104
1201	13	-0.8573586298928788
1201	14	0.025668877829374992
1201	15	-0.3130896703739552
1201	16	0.1594739952471187
1201	17	-0.4532935047355906
1201	18	-0.2274685336962392
1201	19	-0.7559501571529097
1201	20	-0.9602331221750049
1201	21	-1.1179259425219712
1201	22	-1.8129796020189062
1201	23	-0.9618985738643318
1201	24	-1.2750359461533307
1201	25	-0.2943354040701749
1202	1	0.059261430854771646
1202	2	-0.008845109575155163
1202	3	-0.6914894402216412
1202	4	-0.7775815898635976
1202	5	-0.35985803386472526
1202	6	-0.994269709739946
1202	7	-1.3057362746086127
1202	8	-0.6829261739696548
1202	9	-0.1845211127266353
1202	10	-0.23900287632201866
1202	11	-1.1282794919228678
1202	12	-0.7806586116902778
1202	13	-0.878239076146464
1202	14	-0.1925581687558804
1202	15	-0.41063226045385903
1202	16	0.11884381282589374
1202	17	-0.10895459323249668
1202	18	0.3949496782742159
1202	19	-0.2166014461624144
1202	20	-0.010210507973283711
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2211	15	-0.546618288963252
2211	16	-0.2913327188459647
2211	17	-0.9600354957152675
2211	18	-0.4550456016276101
2211	19	-0.48847910121763505
2211	20	-0.21624279344482034
2211	21	-0.9106910887534312
2211	22	-0.08995919006000719
2211	23	-2.3271517472497436
2211	24	-1.061291583852686
2211	25	-0.6199038236544965
2212	1	-0.0759640635256531
2212	2	-1.8246795099165993
2212	3	-0.7510996265923886
2212	4	-0.3734700519492442
2212	5	0.023126743486251655
2212	6	-0.0757179954135836
2212	7	0.17199882284479173
2212	8	0.7487141731636484
2212	9	0.6559256080017732
2212	10	0.5676552542797779
2212	11	0.19360128770438934
2212	12	-0.0703868802917853
2212	13	-0.20694870767299278
2212	14	0.14696381161117245
2212	15	-0.0959360020956268
2212	16	-0.12607011518662858
2212	17	0.4776153313655982
2212	18	0.8901125748455598
2212	19	-0.3435754111645437
2212	20	-0.06548152803303964
2212	21	-0.33129571338596264
2212	22	-0.14533192491148128
2212	23	-0.05145081267030349
2212	24	0.01022787558305538
2212	25	-0.7772027574441438
2213	1	0.12277676856365716
2213	2	-0.40690403989215085
2213	3	-0.23964379482760734
2213	4	-0.3808260156886689
2213	5	-0.1574188959417171
2213	6	0.594172314185947
2213	7	0.08885479740181523
2213	8	0.4629566170744255
2213	9	0.7912969759946762
2213	10	0.6825164645521653
2213	11	0.09526936065824287
2213	12	-0.2908061702249327
2213	13	-0.31027836302445877
2213	14	-1.4868606404777083
2213	15	-0.3115306452232076
2213	16	0.01925343904196808
2213	17	0.267342944355363
2213	18	0.5976913810159132
2213	19	0.5733021985799454
2213	20	0.26146942499696735
2213	21	0.28638888885111413
2213	22	0.6307006144104719
2213	23	0.48311139931126235
2213	24	0.4792571047213272
2213	25	-0.04610650249509973
2214	1	0.3324866406996989
2214	2	0.2879965464214287
2214	3	0.5728440353794433
2214	4	-0.002578033169900351
2214	5	0.5630328467726358
2214	6	0.17780313134697678
2214	7	0.07810210922976779
2214	8	-0.3076238550053674
2214	9	0.045596969504117624
2214	10	0.2765754420732023
2214	11	-0.11625786722518683
2214	12	0.15496523238427073
2214	13	-0.46046983443356027
2214	14	-0.1488108362761551
2214	15	-0.6546214481050185
2214	16	-0.6941452325575241
2214	17	-0.5552485183996652
2214	18	-0.2507324955769761
2214	19	-0.8459228290049197
2214	20	-0.6758101665306882
2214	21	-0.11533327207141042
2214	22	-0.32843051646077726
2214	23	0.08469942207148051
2214	24	-0.3184106220695213
2214	25	-0.861709799697671
2215	1	0.5066520032453461
2215	2	0.7100101092800628
2215	3	0.4583422096100094
2215	4	0.6441355818535719
2215	5	0.23315543414874185
2215	6	0.16397944850920965
2215	7	-0.01912011299245364
2215	8	0.5426630516195701
2215	9	0.9026000993945758
2215	10	0.8359129485938084
2215	11	0.007242393599014463
2215	12	0.5274221963905319
2215	13	0.016121193213805896
2215	14	0.2851354339377181
2215	15	0.5043509045293106
2215	16	0.2762512202332985
2215	17	0.5564494471715449
2215	18	0.8271887355132677
2215	19	0.8507386211776549
2215	20	-0.08302195578473004
2215	21	0.9280939579755412
2215	22	-0.3326037037325591
2215	23	0.2726232614037727
2215	24	0.010619111889788257
2215	25	0.8616209253936233
2216	1	-0.027078155484815625
2216	2	-0.8349634356934814
2216	3	-0.2585890073212056
2216	4	0.5131832009011923
2216	5	0.36741469340800137
2216	6	0.3147730993028617
2216	7	0.5595835907112487
2216	8	-0.11342160446508842
2216	9	1.3159598877543601
2216	10	0.38485474753560567
2216	11	1.2096233459799706
2216	12	0.12001478898312001
2216	13	0.3091106112032236
2216	14	-0.1586476348453445
2216	15	-0.19803004785164546
2216	16	0.37016656414864
2216	17	0.6721901879122854
2216	18	0.7193845027090404
2216	19	0.18671745715649024
2216	20	-0.3918843896471671
2216	21	-0.6287843489027622
2216	22	-0.2744026455315023
2216	23	-0.7974825587020504
2216	24	-0.12033326906259134
2216	25	-0.5173473285746368
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	231.0
3	27.0
4	28.0
5	21.0
6	24.0
7	26.0
8	17.0
9	44.0
10	114.0
11	240.0
12	531.0
13	929.0
14	1191.0
15	1534.0
16	1752.0
17	1836.0
18	1863.0
19	1665.0
20	1511.0
21	1451.0
22	1426.0
23	1449.0
24	1464.0
25	1443.0
26	1600.0
27	1688.0
28	1914.0
29	2103.0
30	2497.0
31	3098.0
32	4014.0
33	5172.0
34	6865.0
35	9437.0
36	14495.0
37	23056.0
38	30474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63172392082792	23.61232440089718	12.839885098178097	20.9160665800968
2	15.77993507107511	28.034481671762222	33.905663945535345	22.27991931162732
3	18.93724377168086	33.240302743614	25.02286345001577	22.799590034689373
4	12.940953793010987	25.36770794722356	30.621692600099372	31.069645659666083
5	14.117257317736884	41.64504144942855	27.773877789258645	16.463823443575933
6	27.009854158454864	40.34607804493496	17.42767047694127	15.2163973196689
7	24.100061493826967	38.59683700982324	18.723293546301697	18.579807950048092
8	23.452370626779054	39.663941083242	19.472020059452937	17.411668230526008
9	25.359308110153655	23.83614131077491	17.463596155817125	33.34095442325431
10	17.221284716198525	35.38804863816298	24.20868358383144	23.181983061807056
11	29.649623301705386	33.437884184976205	17.961731236011726	18.950761277306686
12	21.08765235489822	34.61392914019016	23.362923952633984	20.935494552277635
13	27.102266009852215	29.351724137931036	22.855566502463056	20.690443349753696
14	21.707051757027322	31.371096151723922	22.95249956645804	23.969352524790718
15	25.825934745241515	32.73697822213111	21.227508555028148	20.20957847759923
16	20.579819811298446	34.703271930446846	20.64681910977638	24.070089148478328
17	21.09698986108264	34.224759141582176	21.272804679985494	23.40544631734969
18	19.921172946555256	32.181932839350466	26.604130537600508	21.292763676493774
19	24.12724692526017	32.852412488174075	23.78902554399243	19.23131504257332
20	22.876666981935116	30.135723068192565	26.01595258362496	20.97165736624736
21	25.339997950157283	30.879303684198078	22.982678829066767	20.798019536577865
22	23.934048958875177	31.40792232152709	23.071042385838812	21.586986333758926
23	24.0384236530839	30.511973900914885	23.954893972466724	21.494708473534487
24	24.085663839581297	30.775294006368824	23.39754705678343	21.74149509726645
25	23.603360761688577	31.977678993663105	24.054982817869416	20.363977426778902
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6248.0
1	6248.0
2	3766.5
3	1285.0
4	1285.0
5	1285.0
6	1130.0
7	975.0
8	975.0
9	975.0
10	1077.0
11	1179.0
12	1179.0
13	1179.0
14	1295.5
15	1412.0
16	1412.0
17	1412.0
18	1750.0
19	2088.0
20	2088.0
21	2088.0
22	2734.0
23	3380.0
24	3380.0
25	3380.0
26	4566.0
27	5752.0
28	5752.0
29	5752.0
30	6110.5
31	6469.0
32	6469.0
33	6469.0
34	6671.0
35	6873.0
36	6873.0
37	6873.0
38	7161.5
39	7450.0
40	7450.0
41	7450.0
42	9874.0
43	12298.0
44	12298.0
45	12298.0
46	15359.0
47	18420.0
48	18420.0
49	18420.0
50	17242.0
51	16064.0
52	16064.0
53	16064.0
54	13827.0
55	11590.0
56	11590.0
57	11590.0
58	10422.0
59	9254.0
60	9254.0
61	9254.0
62	8675.0
63	8096.0
64	8096.0
65	8096.0
66	5824.0
67	3552.0
68	3552.0
69	3552.0
70	3214.5
71	2877.0
72	2877.0
73	2877.0
74	2094.5
75	1312.0
76	1312.0
77	1312.0
78	895.0
79	478.0
80	478.0
81	478.0
82	309.0
83	140.0
84	140.0
85	140.0
86	84.5
87	29.0
88	29.0
89	29.0
90	19.0
91	9.0
92	9.0
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12968639471822682
2	0.25308496423799415
3	0.30653147842489975
4	0.33875658256700464
5	0.35290418926353845
6	0.29867169692682544
7	0.30495952212528493
8	0.31989310697162615
9	0.30574550027509234
10	0.3269669103198931
11	0.26566061463491314
12	0.30495952212528493
13	0.27902224318163954
14	0.2892399591291362
15	0.3175351725222039
16	0.28531006838009904
17	0.3081034347245147
18	0.2908119154287511
19	0.30653147842489975
20	0.27823626503183213
21	0.3073174565747072
22	0.2735203961329875
23	0.25858681128664623
24	0.2845240902302916
25	0.27823626503183213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	127230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.96030810343472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41187050359711	30.209856165998584
2	5.334982014388489	3.730252298986088
3	1.7221223021582732	1.8061777882574865
4	0.9554856115107914	1.33616285467264
5	0.7261690647482014	1.269354711939008
6	0.5238309352517986	1.0987974534307947
7	0.39568345323741005	0.9683250805627603
8	0.3125	0.874007702585868
9	0.3237410071942446	1.0186276821504363
>10	2.5719424460431655	19.441169535486914
>50	0.4226618705035971	10.266446592784721
>100	0.2720323741007194	17.342607875501063
>500	0.020233812949640287	4.341743299536273
>1k	0.0044964028776978415	1.6922109565354084
>5k	0.0022482014388489208	4.604260001571956
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	5858	4.604260001571956	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1132	0.8897272655820169	No Hit
ATACAGGGTGACAGCCCCGTACACA	1021	0.8024836909533916	No Hit
GTATCAACGCAGAGTACTTTTTTTT	881	0.6924467499803505	No Hit
GGATACCACGTGTCCCGCCCTACTC	837	0.6578637113888234	No Hit
GGTATCAACGCAGAGTACTTTTTTT	636	0.4998821032775289	No Hit
TATCAACGCAGAGTACTTTTTTTTT	587	0.4613691739369646	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	536	0.4212842882967854	No Hit
GTACATAAGCAGTGGTATCAACGCA	529	0.4157824412481333	No Hit
GTGGTATCAACGCAGAGTACATGGG	511	0.4016348345515995	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	505	0.3969189656527548	No Hit
CCCATATTCAGACAGGATACCACGT	502	0.3945610312033326	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	481	0.37805549005737643	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	470	0.3694097304094946	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	456	0.35840603631219053	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	446	0.35054625481411616	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	380	0.29867169692682544	No Hit
CACACACACTGATTCAGGCTCTGGG	373	0.29316984987817335	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	352	0.27666430873221726	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	336	0.2640886583352983	No Hit
TATTCAGACAGGATACCACGTGTCC	335	0.26330268018549086	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	331	0.2601587675862611	No Hit
ATCATTAACTGAATCCATAGGTTAA	327	0.25701485498703136	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	308	0.24208127014069009	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	305	0.23972333569126777	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	305	0.23972333569126777	No Hit
CGGTACTGGTTCACTATCGGTCAGT	303	0.2381513793916529	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	298	0.23422148864261574	No Hit
GCCCTACTCATCGAGCTCACAGCAT	298	0.23422148864261574	No Hit
TCACGGTACTGGTTCACTATCGGTC	297	0.23343551049280828	No Hit
GCTTATGTACTCTGCGTTGATACCA	293	0.23029159789357856	No Hit
TTCCAGACGCTTCCACTAACACACA	292	0.22950561974377112	No Hit
AGCTCACAGCATGTGCATTTTTGTG	286	0.2247897508449265	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG	280	0.22007388194608188	No Hit
GGGTGACAGCCCCGTACACAAAAAT	265	0.20828420969897038	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	261	0.2051402970997406	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	252	0.1980664937514737	No Hit
CTACTGGGGGAATCTCGGTTGATTT	249	0.19570855930205142	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	242	0.19020671225339936	No Hit
GTCCCCCCATATTCAGACAGGATAC	234	0.18391888705493986	No Hit
GTTAATGATAGTGTGTCGAAACACA	233	0.18313290890513242	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	231	0.18156095260551758	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	226	0.1776310618564804	No Hit
CAGTAGCGGCGAGCGAACGGGGAGC	225	0.17684508370667296	No Hit
TTTGTGTACGGGGCTGTCACCCTGT	221	0.1737011711074432	No Hit
GGAGTATTTAGCCTTGGAGGATGGT	214	0.16819932405879118	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA	211	0.16584138960936887	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA	207	0.16269747701013912	No Hit
CACTGATTCAGGCTCTGGGCTGCTC	193	0.15169378291283503	No Hit
TCCCCCAGTAGCGGCGAGCGAACGG	193	0.15169378291283503	No Hit
TAAGTACCCCGAGGAAAAGAAATCA	190	0.14933584846341272	No Hit
CTTATGTACTCTGCGTTGATACCAC	190	0.14933584846341272	No Hit
GGTATCAACGCAGAGTACATGGGGA	188	0.14776389216379784	No Hit
GTATCGCGCGCCTTTCCAGACGCTT	184	0.1446199795645681	No Hit
AACACACACACTGATTCAGGCTCTG	178	0.13990411066572347	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	177	0.13911813251591607	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	172	0.13518824176687888	No Hit
AGTCAGGAGTATTTAGCCTTGGAGG	167	0.1312583510178417	No Hit
GTACGGGGCTGTCACCCTGTATCGC	165	0.12968639471822682	No Hit
CTAACACACACACTGATTCAGGCTC	163	0.12811443841861198	No Hit
CTACTCATCGAGCTCACAGCATGTG	162	0.12732846026880454	No Hit
GTACATGGGTAGGTTAATGAGGCGA	159	0.12497052581938223	No Hit
GTTATAACCGGCGATTTCCGAATGG	158	0.12418454766957479	No Hit
GGTTAGAAGAGAACGAAAAACAATA	156	0.1226125913699599	No Hit
GTACACAAAAATGCACATGCTGTGA	156	0.1226125913699599	No Hit
CCTATATATTTTTCTTTCTCTTCTT	156	0.1226125913699599	No Hit
GTGCATTTTTGTGTACGGGGCTGTC	155	0.12182661322015248	No Hit
GTACATGGGAGAGCTGATGACGAGT	153	0.1202546569205376	No Hit
GGCTCTGGGCTGCTCCCCGTTCGCT	153	0.1202546569205376	No Hit
GCATGTGCATTTTTGTGTACGGGGC	149	0.11711074432130787	No Hit
GTACATGGGATATCAATAAGCGGAG	149	0.11711074432130787	No Hit
GTACATGGGCATCTAAGTACCCCGA	147	0.11553878802169301	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	146	0.11475280987188556	No Hit
GGGCTGTCACCCTGTATCGCGCGCC	146	0.11475280987188556	No Hit
GGAGGATGGTCCCCCCATATTCAGA	145	0.11396683172207812	No Hit
CAGCATGTGCATTTTTGTGTACGGG	145	0.11396683172207812	No Hit
TCATTAACCTATGGATTCAGTTAAT	140	0.11003694097304094	No Hit
GGTATCAACGCAGAGTACATGGGGG	136	0.1068930283738112	No Hit
GTACATGGGAGTGTACCTGACGTTT	135	0.10610705022400377	No Hit
AAGTACCCCGAGGAAAAGAAATCAA	135	0.10610705022400377	No Hit
CCGCTACTGGGGGAATCTCGGTTGA	135	0.10610705022400377	No Hit
TTTCCAGACGCTTCCACTAACACAC	134	0.10532107207419635	No Hit
CCACGTGTCCCGCCCTACTCATCGA	132	0.10374911577458147	No Hit
GGGCTGCTCCCCGTTCGCTCGCCGC	130	0.10217715947496661	No Hit
AAAATGCACATGCTGTGAGCTCGAT	130	0.10217715947496661	No Hit
GAGTACATAAGCAGTGGTATCAACG	130	0.10217715947496661	No Hit
CTGTGAGCTCGATGAGTAGGGCGGG	130	0.10217715947496661	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	130	0.10217715947496661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.859781498074353E-4	0.0	0.0	0.0	0.0
6	7.859781498074353E-4	0.0	0.0	0.0	0.0
7	7.859781498074353E-4	0.0	0.0	0.0	0.0
8	7.859781498074353E-4	0.0	0.0	0.0	0.0
9	7.859781498074353E-4	0.0	0.0	0.0	0.0
10	7.859781498074353E-4	0.0	0.0	0.0	0.0
11	7.859781498074353E-4	0.0	0.0	0.0	0.0
12	7.859781498074353E-4	0.0	0.0	7.859781498074353E-4	0.0
13	7.859781498074353E-4	0.0	0.0	7.859781498074353E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAAAA	40	2.711893E-4	16.644352	1
CTGACAC	35	0.002144398	16.298235	3
CGAATGG	35	0.0021557468	16.285376	19
CTGAACC	35	0.0021614402	16.278954	9
AATAACT	40	0.0052170907	14.260956	4
TAGTGTG	40	0.0052582696	14.244085	9
GTCACAG	40	0.0052582696	14.244085	15
ACTGAAC	40	0.0052582696	14.244085	8
ACCGACT	40	0.0052582696	14.244085	13
GTGTGTC	40	0.0052582696	14.244085	11
AAATAAC	50	0.001477744	13.3102255	3
ACAGGGT	195	0.0	12.676405	3
TACAGGG	225	0.0	12.253859	2
TGTGACT	55	0.0030422732	12.090658	19
GAACCGA	55	0.0030512854	12.085891	11
ATAGGTT	95	1.0152435E-6	11.999751	17
CCCCGTA	245	0.0	11.627825	15
ACGCTTC	90	7.344408E-6	11.606292	7
CAGGGTG	215	0.0	11.497205	4
AGGGTGA	225	0.0	11.408765	5
>>END_MODULE