FastQCFastQC Report
Wed 25 May 2016
SRR1295205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127230
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36152.841311011553879No Hit
GGTATCAACGCAGAGTACTTTTTTT26272.0647645995441324No Hit
TATCAACGCAGAGTACTTTTTTTTT22981.8061777882574865No Hit
GTACTGGTTCACTATCGGTCAGTCA12560.9871885561581387No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12430.9769708402106422No Hit
ATACAGGGTGACAGCCCCGTACACA11930.9376719327202705No Hit
GGATACCACGTGTCCCGCCCTACTC9400.7388194608189892No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9020.7089522911263066No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6040.474730802483691No Hit
ACCCTGTATCGCGCGCCTTTCCAGA5970.4692289554350389No Hit
GTGGTATCAACGCAGAGTACATGGG5910.4645130865361943No Hit
CCCATATTCAGACAGGATACCACGT5750.4519374361392753No Hit
GTACATAAGCAGTGGTATCAACGCA5100.400848856401792No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4940.3882732060048731No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4910.38591527155545075No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4870.382771358956221No Hit
GTATTTAGCCTTGGAGGATGGTCCC4750.37333962115853175No Hit
GCCTTGGAGGATGGTCCCCCCATAT4750.37333962115853175No Hit
GTTCACTATCGGTCAGTCAGGAGTA4420.3474023422148864No Hit
CATCTAAGTACCCCGAGGAAAAGAA4310.33875658256700464No Hit
CACACACACTGATTCAGGCTCTGGG4140.32539495402027824No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4100.3222510414210485No Hit
ATCAACGCAGAGTACTTTTTTTTTT4030.31674919437239646No Hit
CGGTACTGGTTCACTATCGGTCAGT3970.31203332547355184No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3890.30574550027509234No Hit
TATTCAGACAGGATACCACGTGTCC3890.30574550027509234No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3820.3002436532264403No Hit
GTGGTATCAACGCAGAGTACTTTTT3760.2955277843275957No Hit
CTATCGGTCAGTCAGGAGTATTTAG3670.2884539809793288No Hit
ATCATTAACTGAATCCATAGGTTAA3600.28295213393067675No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3400.267232570934528No Hit
TCACGGTACTGGTTCACTATCGGTC3350.26330268018549086No Hit
AGCTCACAGCATGTGCATTTTTGTG3210.25229898608818674No Hit
GGTTAATGAGGCGAACCGGGGGAAC3100.24365322644030496No Hit
GCTTATGTACTCTGCGTTGATACCA2990.23500746679242318No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2950.2318635541931934No Hit
GGTTCACTATCGGTCAGTCAGGAGT2890.2271476852943488No Hit
TTCCAGACGCTTCCACTAACACACA2880.22636170714454137No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2790.21928790379627444No Hit
GTCCCGCCCTACTCATCGAGCTCAC2750.21614399119704472No Hit
GGGTGACAGCCCCGTACACAAAAAT2730.21457203489742985No Hit
GTTAATGATAGTGTGTCGAAACACA2720.2137860567476224No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2690.2114281222982001No Hit
CTACTGGGGGAATCTCGGTTGATTT2570.20199638450051088No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2560.20121040635070345No Hit
GCCCTACTCATCGAGCTCACAGCAT2530.19885247190128114No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2530.19885247190128114No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2500.19649453745185885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2460.19335062485262908No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2430.1909926904032068No Hit
GGTATCAACGCAGAGTACATGGGGA2400.18863475595378448No Hit
GTCCCCCCATATTCAGACAGGATAC2400.18863475595378448No Hit
TAAGTACCCCGAGGAAAAGAAATCA2340.18391888705493986No Hit
GGAGTATTTAGCCTTGGAGGATGGT2280.17920301815609527No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2280.17920301815609527No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2270.17841704000628783No Hit
CTTATGTACTCTGCGTTGATACCAC2210.1737011711074432No Hit
GTATCGCGCGCCTTTCCAGACGCTT2060.16191149886033168No Hit
GGTTAGAAGAGAACGAAAAACAATA2050.16112552071052424No Hit
CACTGATTCAGGCTCTGGGCTGCTC2050.16112552071052424No Hit
TTTGTGTACGGGGCTGTCACCCTGT1880.14776389216379784No Hit
CTAACACACACACTGATTCAGGCTC1850.14540595771437556No Hit
GTTATAACCGGCGATTTCCGAATGG1810.1422620451151458No Hit
AACACACACACTGATTCAGGCTCTG1800.14147606696533838No Hit
GGTATCAACGCAGAGTACATGGGGG1800.14147606696533838No Hit
GTACATGGGTAGGTTAATGAGGCGA1750.1375461762163012No Hit
GTACGGGGCTGTCACCCTGTATCGC1700.133616285467264No Hit
GGGCTGTCACCCTGTATCGCGCGCC1700.133616285467264No Hit
GTACATGGGAGTGTACCTGACGTTT1690.13283030731745657No Hit
CAACGCAGAGTACTTTTTTTTTTTT1680.13204432916764916No Hit
AAGTACCCCGAGGAAAAGAAATCAA1630.12811443841861198No Hit
CCACGTGTCCCGCCCTACTCATCGA1610.12654248211899707No Hit
CCTATATATTTTTCTTTCTCTTCTT1600.12575650396918966No Hit
GTACACAAAAATGCACATGCTGTGA1590.12497052581938223No Hit
TCATTAACCTATGGATTCAGTTAAT1580.12418454766957479No Hit
GAGTACATAAGCAGTGGTATCAACG1580.12418454766957479No Hit
AAAATGCACATGCTGTGAGCTCGAT1560.1226125913699599No Hit
AACGCAGAGTACTTTTTTTTTTTTT1560.1226125913699599No Hit
GGAGGATGGTCCCCCCATATTCAGA1560.1226125913699599No Hit
CTACTCATCGAGCTCACAGCATGTG1550.12182661322015248No Hit
AACGGGGAGCAGCCCAGAGCCTGAA1530.1202546569205376No Hit
CAGGGTGACAGCCCCGTACACAAAA1530.1202546569205376No Hit
GTGCATTTTTGTGTACGGGGCTGTC1520.11946867877073017No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1490.11711074432130787No Hit
CTGTGAGCTCGATGAGTAGGGCGGG1460.11475280987188556No Hit
GTACATGGGATATCAATAAGCGGAG1460.11475280987188556No Hit
GGCTCTGGGCTGCTCCCCGTTCGCT1460.11475280987188556No Hit
ATTCAGACAGGATACCACGTGTCCC1450.11396683172207812No Hit
CCTTTCCAGACGCTTCCACTAACAC1450.11396683172207812No Hit
CTATCATTAACTGAATCCATAGGTT1450.11396683172207812No Hit
GTACATGGGAGAGCTGATGACGAGT1450.11396683172207812No Hit
GCATGTGCATTTTTGTGTACGGGGC1440.11318085357227069No Hit
GGGCTGCTCCCCGTTCGCTCGCCGC1430.11239487542246325No Hit
GTACTTAGATGTTTCAGTTCCCCCG1430.11239487542246325No Hit
GGTATCAACGCAGAGTACATGGGCA1410.11082291912284839No Hit
GATATGAACCGTTATAACCGGCGAT1400.11003694097304094No Hit
CCGTTATAACCGGCGATTTCCGAAT1390.1092509628232335No Hit
CACTATCATTAACTGAATCCATAGG1390.1092509628232335No Hit
CAGCATGTGCATTTTTGTGTACGGG1390.1092509628232335No Hit
AGATTCCCCCAGTAGCGGCGAGCGA1370.10767900652361864No Hit
GTATCAACGCAGAGTACATGGGGAA1370.10767900652361864No Hit
GGATGGTCCCCCCATATTCAGACAG1360.1068930283738112No Hit
ATCCATAGGTTAATGAGGCGAACCG1360.1068930283738112No Hit
CCTATGGATTCAGTTAATGATAGTG1350.10610705022400377No Hit
ACCCTGTATCGCACGCCTTTCCAGA1340.10532107207419635No Hit
GTACATGGGTTACCGGATAAGGCGC1320.10374911577458147No Hit
GCCCCGTACACAAAAATGCACATGC1320.10374911577458147No Hit
CACTAACACACACACTGATTCAGGC1320.10374911577458147No Hit
CCGCTACTGGGGGAATCTCGGTTGA1310.10296313762477402No Hit
GTACATGGGCATCTAAGTACCCCGA1300.10217715947496661No Hit
CTGTCACCCTGTATCGCGCGCCTTT1290.10139118132515916No Hit
GGTATCAACGCAGAGTACATGGGGT1290.10139118132515916No Hit
GAGTAGGGCGGGACACGTGGTATCC1280.10060520317535172No Hit
ACGCAGAGTACATGGGTAGGTTAAT1280.10060520317535172No Hit
GAAATCAACCGAGATTCCCCCAGTA1280.10060520317535172No Hit
GTATCAACGCAGAGTACATGGGATA1280.10060520317535172No Hit
GCTGTCACCCTGTATCGCGCGCCTT1280.10060520317535172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAAC307.6610316E-419.00314513
CGAAAAA359.865887E-518.99567614
GCTAACA307.6813385E-418.99567414
GAGAACG402.732734E-416.6277529
AGAACGA402.732734E-416.62775210
AAACAAT402.7409534E-416.62121618
ATATTTG402.7409534E-416.6212165
TATTTGA350.002153114616.2884126
AAGCTAA350.002153114616.28841212
GAACGAA456.693715E-414.78022511
AACAATA456.713726E-414.77441519
GAAGCTA400.005238156814.25235911
AACAATG400.005251853314.24675717
TAACAAT400.005251853314.24675716
ACAGGGT1700.013.9674093
TAGAAGA655.387642E-513.1508534
GGTATCA6800.013.1448661
TACAGGG1850.012.8349162
CAGGGTG1900.012.4971544
GAAGAGA550.003038121612.0929116
Produced by FastQC (version 0.11.5)