FastQCFastQC Report
Wed 25 May 2016
SRR1295204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345494
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA203065.877381372759006No Hit
GTATCAACGCAGAGTACTTTTTTTT27550.7974089275067006No Hit
GTACTGGTTCACTATCGGTCAGTCA25620.7415468864871748No Hit
ATACAGGGTGACAGCCCCGTACACA24190.7001568768198579No Hit
GGATACCACGTGTCCCGCCCTACTC21790.6306911263292561No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18590.5380701256751202No Hit
GGTATCAACGCAGAGTACTTTTTTT18490.5351757194046786No Hit
GTACATAAGCAGTGGTATCAACGCA17660.511152147360012No Hit
TATCAACGCAGAGTACTTTTTTTTT16420.4752615096065344No Hit
GTGGTATCAACGCAGAGTACATGGG14090.40782184350524175No Hit
TCTAAGTACCCCGAGGAAAAGAAAT13720.3971125403046073No Hit
GTATTTAGCCTTGGAGGATGGTCCC12470.3609324619240855No Hit
CCCATATTCAGACAGGATACCACGT12030.3481970743341418No Hit
GGTTAATGAGGCGAACCGGGGGAAC11830.34240826179325834No Hit
CATCTAAGTACCCCGAGGAAAAGAA11420.3305411960844472No Hit
GCTTATGTACTCTGCGTTGATACCA10650.3082542678020458No Hit
TATTCAGACAGGATACCACGTGTCC9940.2877039832819094No Hit
GCCTTGGAGGATGGTCCCCCCATAT9940.2877039832819094No Hit
GTTCACTATCGGTCAGTCAGGAGTA9760.28249405199511424No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9200.26628537688064047No Hit
CACACACACTGATTCAGGCTCTGGG8700.25181334552843176No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8610.24920837988503416No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG8500.24602453298754826No Hit
CTATCGGTCAGTCAGGAGTATTTAG8200.23734131417622303No Hit
GTCAGGAGTATTTAGCCTTGGAGGA7870.22778977348376525No Hit
TTCCAGACGCTTCCACTAACACACA7740.22402704533219098No Hit
CTTATGTACTCTGCGTTGATACCAC7500.2170804702831308No Hit
CGGTACTGGTTCACTATCGGTCAGT7450.21563326714790998No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7030.20347676081205462No Hit
TCACGGTACTGGTTCACTATCGGTC7030.20347676081205462No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6970.20174011704978956No Hit
GTCCCGCCCTACTCATCGAGCTCAC6770.1959513045089061No Hit
GGTATCAACGCAGAGTACATGGGGG6570.1901624919680226No Hit
AGCTCACAGCATGTGCATTTTTGTG6440.18639976381644832No Hit
GGGTGACAGCCCCGTACACAAAAAT6270.18147927315669737No Hit
GTATCGCGCGCCTTTCCAGACGCTT6250.18090039190260904No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6130.17742710437807893No Hit
GCCTGAATCAGTGTGTGTGTTAGTG6050.17511157936172553No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6030.1745326981076372No Hit
GCCCTACTCATCGAGCTCACAGCAT5920.17134885121015125No Hit
GGTTCACTATCGGTCAGTCAGGAGT5850.16932276682084205No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC5760.16671780117744447No Hit
GGAGTATTTAGCCTTGGAGGATGGT5700.1649811574151794No Hit
GTATCAACGCAGAGTACATGGGGGT5690.16469171678813524No Hit
CTACTGGGGGAATCTCGGTTGATTT5670.1641128355340469No Hit
CAGTAGCGGCGAGCGAACGGGGAGC5600.1620867511447377No Hit
GAATCAGTGTGTGTGTTAGTGGAAG5450.15774514173907506No Hit
GAGTACATAAGCAGTGGTATCAACG5180.14993024480888234No Hit
TTTGTGTACGGGGCTGTCACCCTGT5170.14964080418183817No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA5110.14790416041957313No Hit
GGTATCAACGCAGAGTACATGGGCA5030.14558863540321973No Hit
TCCCCCAGTAGCGGCGAGCGAACGG4980.14414143226799886No Hit
CACTGATTCAGGCTCTGGGCTGCTC4840.14008926348938042No Hit
TAAGTACCCCGAGGAAAAGAAATCA4830.13979982286233625No Hit
GTCCCCCCATATTCAGACAGGATAC4620.1337215696944086No Hit
GTTAATGATAGTGTGTCGAAACACA4530.13111660405101103No Hit
GGGCTGTCACCCTGTATCGCGCGCC4480.12966940091579013No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA4360.12619611339126005No Hit
AACACACACACTGATTCAGGCTCTG4350.12590667276421588No Hit
CATGTACTCTGCGTTGATACCACTG4240.12272282586672995No Hit
CTGTCACCCTGTATCGCGCGCCTTT4230.12243338523968579No Hit
TATGTACTCTGCGTTGATACCACTG4200.12156506335855326No Hit
AAGTACCCCGAGGAAAAGAAATCAA4190.1212756227315091No Hit
CCCATGTACTCTGCGTTGATACCAC4120.11924953834219985No Hit
GTACGGGGCTGTCACCCTGTATCGC4030.11664457269880228No Hit
GTACATGGGGGTTAATGAGGCGAAC4010.11606569144471394No Hit
GTATCAACGCAGAGTACATGGGCAT3980.11519736956358143No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3970.11490792893653724No Hit
CGTCTGGAAAGGCGCGCGATACAGG3900.11288184454722804No Hit
CTAACACACACACTGATTCAGGCTC3900.11288184454722804No Hit
GTGCATTTTTGTGTACGGGGCTGTC3830.1108557601579188No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN3830.1108557601579188No Hit
CCACGTGTCCCGCCCTACTCATCGA3800.10998743827678628No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3790.1096979976497421No Hit
GCGTTGATACCACTGCTTATGTACT3790.1096979976497421No Hit
ATCATGTACTCTGCGTTGATACCAC3780.10940855702269794No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3670.10622471012521201No Hit
GTACATGGGCATCTAAGTACCCCGA3630.1050669476170353No Hit
GGTATCAACGCAGAGTACATGGGGA3530.10217254134659358No Hit
TATCAACGCAGAGTACATGGGGGTT3480.1007253382113727No Hit
GCTGTCACCCTGTATCGCGCGCCTT3460.10014645695728436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCT307.6826254E-419.0085874
TAAGCTG307.6901517E-419.0058215
TGTAAGA502.1540654E-719.0058212
ATCGATT401.28028805E-519.0002928
TCGATTA401.281896E-518.9975289
ACATCGA453.5299054E-516.886696
GTCTTGT402.7437962E-416.6325151
CCTTAAG402.746845E-416.6300952
TTAATCC350.0021590516.2930744
GACCAAA350.0021590516.2930741
CTAGCCT350.0021590516.2930744
TAATCCG350.002161150616.2907035
GTGTAAG601.4518555E-615.8404881
TTAGGAT508.6491E-515.2068694
TAACATC508.6491E-515.2068694
TTAACAT508.6491E-515.2068693
CCGCGAA508.6921646E-515.1980229
CTTAGGA456.720321E-414.7844563
TCTTGTA456.727744E-414.7823052
CAGGCGT456.742611E-414.77800414
Produced by FastQC (version 0.11.5)