##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295204_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345494
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.587234510584842	31.0	28.0	31.0	16.0	34.0
2	28.588713552189038	31.0	28.0	31.0	16.0	34.0
3	28.807278852888906	31.0	28.0	33.0	16.0	34.0
4	31.70886614528762	35.0	32.0	37.0	17.0	37.0
5	32.44541149773947	35.0	32.0	37.0	22.0	37.0
6	32.75130103561856	35.0	33.0	37.0	23.0	37.0
7	32.80652341285232	35.0	33.0	37.0	23.0	37.0
8	32.808315050333725	35.0	33.0	37.0	23.0	37.0
9	33.481377390055975	38.0	32.0	39.0	17.0	39.0
10	33.61279790676539	37.0	32.0	39.0	17.0	39.0
11	34.037259691919395	38.0	33.0	39.0	21.0	39.0
12	34.126511603674736	38.0	33.0	39.0	22.0	39.0
13	33.96650593063845	38.0	33.0	39.0	20.0	39.0
14	34.37512373586806	38.0	32.0	41.0	17.0	41.0
15	34.72055086340139	39.0	33.0	41.0	18.0	41.0
16	34.68032440505479	39.0	33.0	41.0	18.0	41.0
17	34.36797744678634	38.0	32.0	40.0	17.0	41.0
18	34.20471267228953	38.0	32.0	40.0	17.0	41.0
19	34.496222799817076	38.0	32.0	40.0	19.0	41.0
20	34.54325979611802	38.0	33.0	40.0	19.0	41.0
21	34.54908334153415	38.0	33.0	41.0	19.0	41.0
22	34.50813328161994	38.0	32.0	40.0	20.0	41.0
23	34.22146260137658	38.0	32.0	40.0	19.0	41.0
24	34.51167603489496	38.0	32.0	40.0	22.0	41.0
25	33.318468627530436	37.0	30.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.16254528790445733
1101	2	0.07301447077167111
1101	3	-0.3211216870199358
1101	4	-0.25963508386855594
1101	5	0.06115278502434052
1101	6	-0.1890553034716831
1101	7	-0.20556065141886393
1101	8	-0.1241509924005797
1101	9	0.10769255244546372
1101	10	0.2722889733602969
1101	11	0.364109631084645
1101	12	0.3430571409033405
1101	13	0.3967915133017854
1101	14	0.8235506914958606
1101	15	0.038336896516540264
1101	16	0.10423393795114322
1101	17	-0.11764633320927231
1101	18	0.47584254764279876
1101	19	0.040310783067276645
1101	20	0.27512869693757835
1101	21	0.09172974790084254
1101	22	0.08053162456511131
1101	23	0.2597833031795105
1101	24	0.04498060540340276
1101	25	0.08660257984288933
1102	1	0.08388276913140658
1102	2	0.4206883869462352
1102	3	0.5682272975688569
1102	4	0.571374566138644
1102	5	-0.029629297648853026
1102	6	0.33280844418782607
1102	7	0.4122270484227002
1102	8	0.5870257323581711
1102	9	0.5783752544474865
1102	10	0.9004108227049628
1102	11	0.8086292911739434
1102	12	0.6961681880011383
1102	13	0.5513068524923312
1102	14	0.7022846681630028
1102	15	0.560423890611311
1102	16	0.5936621390169634
1102	17	0.6574652900083535
1102	18	1.26266380646463
1102	19	0.8025101205294689
1102	20	0.9060231229781905
1102	21	0.8771827221194783
1102	22	0.6779534883127667
1102	23	0.5659189794001591
1102	24	0.6916319088745269
1102	25	0.4181739466871903
1103	1	-0.20481110244969614
1103	2	-0.5161771973743718
1103	3	-0.42087611035859496
1103	4	-0.3363408061006439
1103	5	-0.36103195963510615
1103	6	-0.662796353237944
1103	7	-0.10524406463858327
1103	8	0.15188605121146992
1103	9	0.4559823920809478
1103	10	0.11685078175132446
1103	11	0.37419898548431263
1103	12	-0.10112594685332965
1103	13	-0.02024499773437327
1103	14	0.21055583972948
1103	15	-0.27808715224374225
1103	16	-0.2792207043353869
1103	17	0.25752379520069013
1103	18	0.3621080071374436
1103	19	-0.011926035679394431
1103	20	0.13591927626179512
1103	21	-0.32504047518928303
1103	22	-0.09875413198853522
1103	23	-2.3726505686472272
1103	24	-0.9019837120118268
1103	25	-0.047073969065330346
1104	1	-0.1647522050437793
1104	2	-0.09288457254218585
1104	3	-0.13998745243992516
1104	4	-0.1377677689866168
1104	5	-0.2661248529701723
1104	6	-0.18978738870630707
1104	7	-0.06355033114934372
1104	8	-0.1145272699959321
1104	9	-2.1244602046017427
1104	10	-0.18961061013295932
1104	11	-0.06644888598076193
1104	12	-0.0967519483257675
1104	13	0.24303939878375047
1104	14	0.5167465633880468
1104	15	-0.04975143703002516
1104	16	-0.016759139223438524
1104	17	0.34309826144295386
1104	18	0.5565993674076779
1104	19	0.2571181806819567
1104	20	0.44640821134473185
1104	21	0.6987350217575852
1104	22	0.6982338719957042
1104	23	0.4907810713856833
1104	24	0.6015999352966475
1104	25	0.7683303009965954
1105	1	-0.16290400913501557
1105	2	-0.24837105410923144
1105	3	-0.028878674909506685
1105	4	-0.3882438194479647
1105	5	-0.24364547015559168
1105	6	-0.33670496196907607
1105	7	-0.14308576190500588
1105	8	-0.40880346327242023
1105	9	-1.683129923591956
1105	10	-0.4115341340264891
1105	11	-0.03101462994961679
1105	12	-0.27687054089657437
1105	13	-0.20623322411427836
1105	14	0.4745005853143738
1105	15	0.014417364862978843
1105	16	-0.014445393966440179
1105	17	-0.9063451795617965
1105	18	0.18578056061415538
1105	19	-0.0030083927348272255
1105	20	0.10939948842010239
1105	21	0.16137906202585128
1105	22	-0.41098820554451976
1105	23	-0.014021437680277415
1105	24	-0.07976828973520611
1105	25	-0.3081773088100448
1106	1	-0.09907845747582655
1106	2	-0.15604757739300013
1106	3	-0.022653846034877745
1106	4	-0.1229612137483791
1106	5	0.25082645303323403
1106	6	0.10578619463935013
1106	7	0.1705062629636842
1106	8	-0.043866170958125394
1106	9	-2.469782144776282
1106	10	-0.09222261694458211
1106	11	0.17719555947505938
1106	12	0.06745896923312245
1106	13	0.061939754156249194
1106	14	-0.3475481720168361
1106	15	0.027372359273648783
1106	16	-0.029540412572018226
1106	17	-0.022840593248758978
1106	18	0.11110941227487814
1106	19	-0.05197245257893712
1106	20	0.25973714518931246
1106	21	0.22900995187777085
1106	22	0.10602096708154818
1106	23	0.6571651846801032
1106	24	0.17535015811558452
1106	25	0.22197827362874278
1107	1	-0.4121465183932358
1107	2	-0.3849438293308687
1107	3	-0.1481554115271706
1107	4	-1.584504059600583
1107	5	-0.8068117814562328
1107	6	-0.5170859517569752
1107	7	-0.2506073644187481
1107	8	-0.19346567851200547
1107	9	-0.11819613389383221
1107	10	0.8055150187060889
1107	11	0.14431642155975055
1107	12	-0.39332772864206333
1107	13	-0.03830874874675061
1107	14	0.7441813169943217
1107	15	-0.656789570385591
1107	16	-0.8737625300903318
1107	17	-0.09597036192067776
1107	18	-0.3300706345379041
1107	19	-0.008324855533615505
1107	20	-0.5033263560418035
1107	21	-0.386665489651854
1107	22	-0.21374603294884764
1107	23	0.25699191660095266
1107	24	-0.2713433093738544
1107	25	-0.13640926930830233
1108	1	0.1483342635378193
1108	2	0.38362042902337734
1108	3	0.2521979103589729
1108	4	0.6102291328442675
1108	5	0.3562281075940916
1108	6	0.12188842415109491
1108	7	0.29282481098740476
1108	8	0.04955703975506509
1108	9	0.6808297543639981
1108	10	0.5929638365637047
1108	11	0.15980644929717158
1108	12	-0.08788591118069178
1108	13	0.11644293531083605
1108	14	0.1657009313121094
1108	15	0.2554314043865631
1108	16	0.4609521707417912
1108	17	0.22370339319753896
1108	18	0.8966761624506034
1108	19	0.3823732028470488
1108	20	0.6209504898173961
1108	21	0.18845338500143072
1108	22	0.31238327625733575
1108	23	0.48849369972074896
1108	24	0.03369235302213269
1108	25	0.0167045861929509
1109	1	0.393849319970883
1109	2	0.06705606791269147
1109	3	0.3912227835559321
1109	4	0.588271263439978
1109	5	0.32692251607504375
1109	6	0.44534051316454537
1109	7	0.4210504823200196
1109	8	0.09663181166190782
1109	9	0.8519049527555396
1109	10	0.33699381234546166
1109	11	0.41257174562787924
1109	12	0.3893809311570493
1109	13	0.059415838300793666
1109	14	0.5136119178626117
1109	15	0.5240649778714044
1109	16	0.03757471655081446
1109	17	0.4842246184271559
1109	18	0.2523400315807294
1109	19	0.16453395711570096
1109	20	0.35267333568887693
1109	21	0.15327144358214895
1109	22	0.18555586547640956
1109	23	0.4971208759350887
1109	24	0.44410162601803194
1109	25	0.28877440783095665
1110	1	-0.08504298178555558
1110	2	0.027059202793971338
1110	3	0.1557268845232258
1110	4	0.2726037899432896
1110	5	-0.02185412664795905
1110	6	-0.06423745344294218
1110	7	-0.10746035864202952
1110	8	-0.1555543158855599
1110	9	0.8104672479709265
1110	10	0.4825969906327927
1110	11	0.03515012519568472
1110	12	0.16558959367803538
1110	13	-0.2976109679484722
1110	14	0.4418412223711101
1110	15	0.1212869907666132
1110	16	0.2792650204233027
1110	17	0.49824115492591403
1110	18	0.47721953458147226
1110	19	0.15455056595787653
1110	20	-0.11407732971981943
1110	21	0.7168417116446406
1110	22	0.29179216921023254
1110	23	0.5180552514154968
1110	24	0.551718483239263
1110	25	-0.019806425384317095
1111	1	0.3555608810055304
1111	2	0.6946933206084829
1111	3	0.31931070312922927
1111	4	0.567300961231652
1111	5	0.3459557413537482
1111	6	0.3747512403742377
1111	7	0.435768617860667
1111	8	0.21059397024069426
1111	9	0.7070438323526744
1111	10	0.47782265016012104
1111	11	0.17712719103393226
1111	12	0.1576189513853663
1111	13	0.2788620228370249
1111	14	0.3908705738900551
1111	15	0.7643841546289138
1111	16	0.31232338309990126
1111	17	0.40826200978967364
1111	18	0.5873387847965219
1111	19	0.01691774770461052
1111	20	0.004136330525362553
1111	21	-0.2933753150829972
1111	22	-0.00703884450941672
1111	23	0.3300365872406843
1111	24	0.5521826122547537
1111	25	0.33594846498841235
1112	1	0.3255562720026113
1112	2	-0.018688151199786063
1112	3	0.06934296614966229
1112	4	0.4033187827096043
1112	5	0.30206881555869103
1112	6	0.7674597977563238
1112	7	0.39206297951376
1112	8	0.2103727381005811
1112	9	-1.5689586257822121
1112	10	-0.21346940699151418
1112	11	0.08682453317558725
1112	12	0.18960543164347143
1112	13	-0.24131004660528532
1112	14	0.2917186304271553
1112	15	0.10007934590252887
1112	16	0.33531923499727156
1112	17	0.5482282104349352
1112	18	1.0551986711077816
1112	19	0.36415080961888435
1112	20	0.13891893922101417
1112	21	0.3047134850399047
1112	22	0.35772379184025027
1112	23	0.3600289055691519
1112	24	0.22961386129454553
1112	25	0.23917476703173435
1113	1	0.33157633889224414
1113	2	-0.06417310103255858
1113	3	0.29656704641721987
1113	4	0.3530177793651248
1113	5	0.23103202626103325
1113	6	-0.17581779421691834
1113	7	-0.09696712082068615
1113	8	-0.012570405712125421
1113	9	-0.46695193681900093
1113	10	-0.06189750063699506
1113	11	-0.36942964742642204
1113	12	-0.9833042673531907
1113	13	-0.24866790613705803
1113	14	-0.610689396328695
1113	15	-0.3244691490807483
1113	16	-0.15458043055456727
1113	17	0.14541884588643939
1113	18	0.13780736675995797
1113	19	0.32221101028778065
1113	20	0.03256442417085026
1113	21	-0.01990189957547983
1113	22	0.2903659323084753
1113	23	-0.2667938369057623
1113	24	-0.08443296144793067
1113	25	-0.010323560727464098
1114	1	0.4166031343582368
1114	2	0.3831951710984569
1114	3	0.25665113947416884
1114	4	0.6005320542464219
1114	5	0.572525668350572
1114	6	0.47912903927770145
1114	7	0.3276151019297089
1114	8	0.14400622592519596
1114	9	0.2811112631311943
1114	10	0.31306925261269924
1114	11	0.3119080320352907
1114	12	-0.01199833500113101
1114	13	-0.010191088317931474
1114	14	0.7758472260278353
1114	15	0.48512458977805295
1114	16	-0.15871151612462597
1114	17	0.27440491903495
1114	18	0.25165272834312447
1114	19	0.31631148055245006
1114	20	0.21818032375717422
1114	21	0.273747546689286
1114	22	0.1641329730364518
1114	23	0.49550746070782736
1114	24	0.4010403308009103
1114	25	0.11652340235874448
1115	1	0.6738938679105395
1115	2	0.6619497305513811
1115	3	0.7518794487734013
1115	4	0.978973965498831
1115	5	0.7790930370119824
1115	6	0.6540071123343338
1115	7	0.40317175038784114
1115	8	0.25009291261540056
1115	9	0.7550221174102063
1115	10	0.679107695460722
1115	11	0.43912481901882217
1115	12	0.38516733927097135
1115	13	0.23249768620650713
1115	14	0.3695040975551862
1115	15	0.20331087892494537
1115	16	-0.015882811038530065
1115	17	0.38630252967669776
1115	18	0.9224470538368337
1115	19	0.41693853340404985
1115	20	0.505866434331594
1115	21	0.7000527358297362
1115	22	-0.15008725073534634
1115	23	0.35194104638456025
1115	24	0.4870683520422503
1115	25	0.24680226816006723
1116	1	0.591173279675246
1116	2	0.6126417720738715
1116	3	0.4439209712647525
1116	4	0.6345751765714951
1116	5	0.931774697911635
1116	6	0.5106247593931528
1116	7	0.3589459718411234
1116	8	0.34719498873996457
1116	9	0.24777730772161988
1116	10	0.3348170380212778
1116	11	-0.2315066688704519
1116	12	-0.08250134585014024
1116	13	-0.033397642513214976
1116	14	0.33965980689774256
1116	15	0.3061439239076478
1116	16	0.03558777719675987
1116	17	0.6029980314068055
1116	18	0.31907335141468707
1116	19	0.34524735693346287
1116	20	0.1640628011135945
1116	21	0.21421796074323396
1116	22	0.2713011575691482
1116	23	0.36794450659216693
1116	24	-0.14236503896120922
1116	25	-0.5633188391063726
1201	1	-0.22166896374803358
1201	2	0.028612190951598393
1201	3	0.17086011544449775
1201	4	0.2169147102736524
1201	5	-0.0682958677426484
1201	6	0.2345648632657955
1201	7	0.21053581603216287
1201	8	0.6763061437585947
1201	9	0.9441015427899302
1201	10	0.9259314108337406
1201	11	0.7406975199067887
1201	12	0.6260236908440007
1201	13	0.9055313119860529
1201	14	1.1069201659357901
1201	15	0.9733598265834971
1201	16	0.7343712253168917
1201	17	0.8623832634266719
1201	18	0.9767875887410966
1201	19	0.7802669741853521
1201	20	1.026209267185628
1201	21	0.7498006919163132
1201	22	0.8400003502980624
1201	23	0.4639561358119977
1201	24	0.6143422264785556
1201	25	0.6818685832714095
1202	1	-0.15726491139065502
1202	2	-0.25588985110171336
1202	3	-0.28075837415762805
1202	4	0.11739527829639584
1202	5	-0.457931184441307
1202	6	-0.06790796948786948
1202	7	0.03736536591454609
1202	8	-0.16256286176865586
1202	9	0.7623587982125528
1202	10	0.8140297757479473
1202	11	0.38815830238120697
1202	12	0.4204978801560557
1202	13	0.667826926261661
1202	14	0.9481847958406888
1202	15	0.2963722520810208
1202	16	0.8032237789658936
1202	17	0.9865119632953494
1202	18	1.0523219139976163
1202	19	0.8090216824531424
1202	20	0.8819558793975446
1202	21	0.48468733270254205
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2211	5	0.03585211928871246
2211	6	-0.2621980908837216
2211	7	-0.13135350052331063
2211	8	-0.029117570031324647
2211	9	-1.5441690667429064
2211	10	-0.20039349379358384
2211	11	-0.5144512410154718
2211	12	-0.4394161144218671
2211	13	-0.5884565444043446
2211	14	-0.38650517063856427
2211	15	-0.1835286547152748
2211	16	-0.34322582639742905
2211	17	0.2149638656978894
2211	18	0.09413604078883964
2211	19	0.12073124054018791
2211	20	0.042324607612393095
2211	21	0.08619776954063951
2211	22	-0.01872535481199833
2211	23	-2.0543598503193365
2211	24	-1.358725006571703
2211	25	-0.5328230706391253
2212	1	-0.13112814089297942
2212	2	-1.4691909610193647
2212	3	-0.6003967558260861
2212	4	0.08159148309410469
2212	5	0.20023208086481503
2212	6	0.005847848628846464
2212	7	-0.03907573684234933
2212	8	-0.43279200605795154
2212	9	0.18636524289568968
2212	10	0.3125081144518447
2212	11	-0.46692583653751996
2212	12	-0.31750784845117863
2212	13	-0.14142835479812987
2212	14	0.06069822226388766
2212	15	-0.08717590402352471
2212	16	0.002024351826612758
2212	17	0.42707766325954566
2212	18	0.15355714493209405
2212	19	0.16043593236363307
2212	20	-0.03667999600524752
2212	21	6.200215675633558E-4
2212	22	0.16061938486006966
2212	23	0.2660537502896503
2212	24	0.24917907868584876
2212	25	0.04207331775637613
2213	1	0.3927593427931946
2213	2	0.5561402240862563
2213	3	0.324626054656342
2213	4	0.6847006384710284
2213	5	0.7050569310086061
2213	6	0.5818009803059709
2213	7	0.2754201803700411
2213	8	0.30342064538632485
2213	9	0.4534817669246607
2213	10	0.42814571074673324
2213	11	0.5083377833416733
2213	12	0.4179511908521505
2213	13	-0.005287310944254386
2213	14	-0.8483832371450006
2213	15	0.22930943374485935
2213	16	0.506837652359053
2213	17	0.46656289708142396
2213	18	-0.1933729683695944
2213	19	-0.0036147473309924294
2213	20	0.2970056004744279
2213	21	0.2730695786399693
2213	22	0.17820655555177467
2213	23	0.3756220258171794
2213	24	0.2462079411646272
2213	25	0.20109991651132475
2214	1	0.5340771371981532
2214	2	0.7909105670290408
2214	3	0.8678543637172105
2214	4	0.9544250386268729
2214	5	1.0462564202488096
2214	6	0.8888085706060807
2214	7	0.4100747776348044
2214	8	0.39411463599585517
2214	9	0.6922022510657939
2214	10	0.42137729026287474
2214	11	0.7204652002792216
2214	12	0.569708847272338
2214	13	0.38903005972345994
2214	14	0.6401943423199512
2214	15	0.47846693602706836
2214	16	0.5095876786648859
2214	17	0.5046509036109583
2214	18	0.8602966246435741
2214	19	0.4659088753096583
2214	20	1.0317745214801377
2214	21	1.0579987519541874
2214	22	1.1710542120572782
2214	23	1.2942303475617223
2214	24	1.2127469178818302
2214	25	0.7221403194314178
2215	1	0.29500113434652775
2215	2	0.2501850246690296
2215	3	0.22419778787374867
2215	4	0.3059635848759932
2215	5	0.25833179133724116
2215	6	0.25954344451426437
2215	7	0.21978074692763272
2215	8	-0.08554722579290797
2215	9	0.2913542973410017
2215	10	0.5337789757375404
2215	11	0.04293104739252129
2215	12	0.09104969221214532
2215	13	-0.1446649435512768
2215	14	0.06846603527144879
2215	15	0.1548679723505515
2215	16	-0.13179976605583477
2215	17	0.46588730476320706
2215	18	0.7511667770202237
2215	19	0.07091777215837425
2215	20	-0.7640297594400351
2215	21	-0.2757258108830669
2215	22	-0.7487031676903655
2215	23	0.12356734396241364
2215	24	0.2517742343951923
2215	25	0.1447261435925924
2216	1	0.37733848900222
2216	2	-0.1923661770517242
2216	3	0.15335393363888983
2216	4	0.5309020181189297
2216	5	0.24837512186500277
2216	6	0.07647664068621651
2216	7	-0.006385904152516275
2216	8	0.01988311804574039
2216	9	0.01709699505601492
2216	10	-0.21298370389711607
2216	11	-0.2006176916579463
2216	12	0.1147131480555359
2216	13	-0.0444436149562506
2216	14	-0.1918849731764496
2216	15	-0.3818164629498
2216	16	0.045166473540163565
2216	17	-0.3701736697060767
2216	18	0.5576663561841642
2216	19	0.02647748411946793
2216	20	-0.7877555613665308
2216	21	-1.0004647366600494
2216	22	-0.8535100506979418
2216	23	-1.6937274594915621
2216	24	-1.2431632636564913
2216	25	-1.076845601162546
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	687.0
3	76.0
4	86.0
5	61.0
6	79.0
7	84.0
8	62.0
9	120.0
10	305.0
11	706.0
12	1404.0
13	2256.0
14	3131.0
15	4026.0
16	4746.0
17	4978.0
18	4742.0
19	4514.0
20	4213.0
21	3892.0
22	3720.0
23	3775.0
24	3892.0
25	4047.0
26	4269.0
27	4433.0
28	4739.0
29	5463.0
30	6448.0
31	8082.0
32	10427.0
33	13660.0
34	18419.0
35	25766.0
36	40702.0
37	67536.0
38	79948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.908234093919276	25.00195628925258	11.682379775157155	20.40742984167099
2	16.289504770031375	28.414410806072905	32.97605261401202	22.3200318098837
3	19.06086653502149	33.788767568823324	23.8790800325241	23.271285863631082
4	12.23110393344913	26.06004449892238	29.88689373122883	31.821957836399655
5	13.755602482956542	43.16823860550096	27.16176688442401	15.914392027118488
6	28.117703771678148	41.223617419182474	15.91579644321574	14.742882365923638
7	24.702267731078283	38.66642660293362	18.227806930678696	18.403498735309405
8	23.799454515352288	39.06024439338796	18.915362741481523	18.224938349778235
9	24.341293280944623	24.516987126432777	17.468324287278314	33.673395305344286
10	17.810260536643323	35.00265794434875	23.2519295514029	23.93515196760503
11	29.232618015784784	33.43599151255845	17.595186192523272	19.73620427913349
12	22.56086051232311	34.08537169706668	21.971349167859724	21.38241862275049
13	27.641636788550027	29.724927784471124	22.58466272807769	20.048772698901164
14	21.90814670278777	32.52815791078319	21.4757968274985	24.087898558930537
15	26.268734750784244	33.35744161728825	19.772278378064367	20.601545253863137
16	20.752005434924616	35.388559367551295	20.15306048385926	23.70637471366483
17	21.814759845366964	34.1490971510228	20.786002863789903	23.25014013982033
18	20.405597289049044	32.07145574233189	25.53567124783307	21.98727572078599
19	23.03675565158802	34.025986849747916	23.32078715643224	19.616470342231825
20	23.064193975427923	30.55049120932717	25.12164108042827	21.26367373481664
21	25.26638921722665	32.0323413499377	21.96081050861536	20.740458924220288
22	24.031073011707534	31.67159681722253	22.30005312370275	21.997277047367184
23	24.197902093842696	30.98939437036693	22.785269378348726	22.027434157441647
24	24.0477586977857	30.885777501611507	23.376152010174277	21.690311790428517
25	23.161458348455504	32.752157994524076	22.75874884224646	21.32763481477395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21244.0
1	21244.0
2	12366.5
3	3489.0
4	3489.0
5	3489.0
6	3192.5
7	2896.0
8	2896.0
9	2896.0
10	2808.5
11	2721.0
12	2721.0
13	2721.0
14	2783.5
15	2846.0
16	2846.0
17	2846.0
18	3640.0
19	4434.0
20	4434.0
21	4434.0
22	5604.0
23	6774.0
24	6774.0
25	6774.0
26	8818.5
27	10863.0
28	10863.0
29	10863.0
30	12516.5
31	14170.0
32	14170.0
33	14170.0
34	15243.0
35	16316.0
36	16316.0
37	16316.0
38	18571.5
39	20827.0
40	20827.0
41	20827.0
42	29596.0
43	38365.0
44	38365.0
45	38365.0
46	44240.5
47	50116.0
48	50116.0
49	50116.0
50	48149.0
51	46182.0
52	46182.0
53	46182.0
54	40057.0
55	33932.0
56	33932.0
57	33932.0
58	29565.0
59	25198.0
60	25198.0
61	25198.0
62	23531.5
63	21865.0
64	21865.0
65	21865.0
66	15744.5
67	9624.0
68	9624.0
69	9624.0
70	8768.0
71	7912.0
72	7912.0
73	7912.0
74	5827.5
75	3743.0
76	3743.0
77	3743.0
78	2596.0
79	1449.0
80	1449.0
81	1449.0
82	928.5
83	408.0
84	408.0
85	408.0
86	246.5
87	85.0
88	85.0
89	85.0
90	56.5
91	28.0
92	28.0
93	28.0
94	16.5
95	5.0
96	5.0
97	5.0
98	3.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13111660405101103
2	0.2741002738108332
3	0.3282256710680938
4	0.3519598024857161
5	0.35514364938320203
6	0.3293834335762705
7	0.33025175545740304
8	0.3510914806045836
9	0.3314095179655797
10	0.35977469941590884
11	0.2850990176385118
12	0.33025175545740304
13	0.30072881149889724
14	0.31751636786745935
15	0.35138092123162773
16	0.305359861531604
17	0.3458815493177885
18	0.32214741790016616
19	0.33748777113350736
20	0.3004393708718531
21	0.33777721176055153
22	0.29378223644983703
23	0.27786300196240743
24	0.3172269272404152
25	0.31172755532657587
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	345494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.02302700165928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.89699700046059	24.349448093024954
2	6.469999768680407	3.7555795597428143
3	2.2030034725104817	1.918134878022626
4	1.2599999787557028	1.4627605362206755
5	0.8359999702958423	1.2131624855641292
6	0.5939999787644709	1.0343806453599766
7	0.46699998330353043	0.9487627187634956
8	0.37699998652125405	0.8753344630745233
9	0.35299998737931737	0.9220615348765782
>10	2.772999900857924	16.815941243547037
>50	0.40499998552017996	8.124125427845508
>100	0.3139999887736704	18.24503504201486
>500	0.03499999874865753	6.996871099403383
>1k	0.014999999463710369	7.444986620287687
>5k	0.0	0.0
>10k+	9.99999964247358E-4	5.893415652251746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	20306	5.877381372759006	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2755	0.7974089275067006	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2562	0.7415468864871748	No Hit
ATACAGGGTGACAGCCCCGTACACA	2419	0.7001568768198579	No Hit
GGATACCACGTGTCCCGCCCTACTC	2179	0.6306911263292561	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1859	0.5380701256751202	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1849	0.5351757194046786	No Hit
GTACATAAGCAGTGGTATCAACGCA	1766	0.511152147360012	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1642	0.4752615096065344	No Hit
GTGGTATCAACGCAGAGTACATGGG	1409	0.40782184350524175	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1372	0.3971125403046073	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1247	0.3609324619240855	No Hit
CCCATATTCAGACAGGATACCACGT	1203	0.3481970743341418	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1183	0.34240826179325834	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	1142	0.3305411960844472	No Hit
GCTTATGTACTCTGCGTTGATACCA	1065	0.3082542678020458	No Hit
TATTCAGACAGGATACCACGTGTCC	994	0.2877039832819094	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	994	0.2877039832819094	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	976	0.28249405199511424	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	920	0.26628537688064047	No Hit
CACACACACTGATTCAGGCTCTGGG	870	0.25181334552843176	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	861	0.24920837988503416	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	850	0.24602453298754826	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	820	0.23734131417622303	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	787	0.22778977348376525	No Hit
TTCCAGACGCTTCCACTAACACACA	774	0.22402704533219098	No Hit
CTTATGTACTCTGCGTTGATACCAC	750	0.2170804702831308	No Hit
CGGTACTGGTTCACTATCGGTCAGT	745	0.21563326714790998	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG	703	0.20347676081205462	No Hit
TCACGGTACTGGTTCACTATCGGTC	703	0.20347676081205462	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	697	0.20174011704978956	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	677	0.1959513045089061	No Hit
GGTATCAACGCAGAGTACATGGGGG	657	0.1901624919680226	No Hit
AGCTCACAGCATGTGCATTTTTGTG	644	0.18639976381644832	No Hit
GGGTGACAGCCCCGTACACAAAAAT	627	0.18147927315669737	No Hit
GTATCGCGCGCCTTTCCAGACGCTT	625	0.18090039190260904	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	613	0.17742710437807893	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	605	0.17511157936172553	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	603	0.1745326981076372	No Hit
GCCCTACTCATCGAGCTCACAGCAT	592	0.17134885121015125	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	585	0.16932276682084205	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	576	0.16671780117744447	No Hit
GGAGTATTTAGCCTTGGAGGATGGT	570	0.1649811574151794	No Hit
GTATCAACGCAGAGTACATGGGGGT	569	0.16469171678813524	No Hit
CTACTGGGGGAATCTCGGTTGATTT	567	0.1641128355340469	No Hit
CAGTAGCGGCGAGCGAACGGGGAGC	560	0.1620867511447377	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	545	0.15774514173907506	No Hit
GAGTACATAAGCAGTGGTATCAACG	518	0.14993024480888234	No Hit
TTTGTGTACGGGGCTGTCACCCTGT	517	0.14964080418183817	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA	511	0.14790416041957313	No Hit
GGTATCAACGCAGAGTACATGGGCA	503	0.14558863540321973	No Hit
TCCCCCAGTAGCGGCGAGCGAACGG	498	0.14414143226799886	No Hit
CACTGATTCAGGCTCTGGGCTGCTC	484	0.14008926348938042	No Hit
TAAGTACCCCGAGGAAAAGAAATCA	483	0.13979982286233625	No Hit
GTCCCCCCATATTCAGACAGGATAC	462	0.1337215696944086	No Hit
GTTAATGATAGTGTGTCGAAACACA	453	0.13111660405101103	No Hit
GGGCTGTCACCCTGTATCGCGCGCC	448	0.12966940091579013	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA	436	0.12619611339126005	No Hit
AACACACACACTGATTCAGGCTCTG	435	0.12590667276421588	No Hit
CATGTACTCTGCGTTGATACCACTG	424	0.12272282586672995	No Hit
CTGTCACCCTGTATCGCGCGCCTTT	423	0.12243338523968579	No Hit
TATGTACTCTGCGTTGATACCACTG	420	0.12156506335855326	No Hit
AAGTACCCCGAGGAAAAGAAATCAA	419	0.1212756227315091	No Hit
CCCATGTACTCTGCGTTGATACCAC	412	0.11924953834219985	No Hit
GTACGGGGCTGTCACCCTGTATCGC	403	0.11664457269880228	No Hit
GTACATGGGGGTTAATGAGGCGAAC	401	0.11606569144471394	No Hit
GTATCAACGCAGAGTACATGGGCAT	398	0.11519736956358143	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	397	0.11490792893653724	No Hit
CGTCTGGAAAGGCGCGCGATACAGG	390	0.11288184454722804	No Hit
CTAACACACACACTGATTCAGGCTC	390	0.11288184454722804	No Hit
GTGCATTTTTGTGTACGGGGCTGTC	383	0.1108557601579188	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	383	0.1108557601579188	No Hit
CCACGTGTCCCGCCCTACTCATCGA	380	0.10998743827678628	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	379	0.1096979976497421	No Hit
GCGTTGATACCACTGCTTATGTACT	379	0.1096979976497421	No Hit
ATCATGTACTCTGCGTTGATACCAC	378	0.10940855702269794	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	367	0.10622471012521201	No Hit
GTACATGGGCATCTAAGTACCCCGA	363	0.1050669476170353	No Hit
GGTATCAACGCAGAGTACATGGGGA	353	0.10217254134659358	No Hit
TATCAACGCAGAGTACATGGGGGTT	348	0.1007253382113727	No Hit
GCTGTCACCCTGTATCGCGCGCCTT	346	0.10014645695728436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.894406270441744E-4	0.0
9	0.0	0.0	0.0	2.894406270441744E-4	0.0
10	0.0	0.0	0.0	2.894406270441744E-4	0.0
11	0.0	0.0	0.0	5.788812540883488E-4	0.0
12	0.0	0.0	0.0	8.683218811325233E-4	0.0
13	2.894406270441744E-4	0.0	0.0	8.683218811325233E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCT	30	7.6826254E-4	19.008587	4
TAAGCTG	30	7.6901517E-4	19.005821	5
TGTAAGA	50	2.1540654E-7	19.005821	2
ATCGATT	40	1.28028805E-5	19.000292	8
TCGATTA	40	1.281896E-5	18.997528	9
ACATCGA	45	3.5299054E-5	16.88669	6
GTCTTGT	40	2.7437962E-4	16.632515	1
CCTTAAG	40	2.746845E-4	16.630095	2
TTAATCC	35	0.00215905	16.293074	4
GACCAAA	35	0.00215905	16.293074	1
CTAGCCT	35	0.00215905	16.293074	4
TAATCCG	35	0.0021611506	16.290703	5
GTGTAAG	60	1.4518555E-6	15.840488	1
TTAGGAT	50	8.6491E-5	15.206869	4
TAACATC	50	8.6491E-5	15.206869	4
TTAACAT	50	8.6491E-5	15.206869	3
CCGCGAA	50	8.6921646E-5	15.198022	9
CTTAGGA	45	6.720321E-4	14.784456	3
TCTTGTA	45	6.727744E-4	14.782305	2
CAGGCGT	45	6.742611E-4	14.778004	14
>>END_MODULE