FastQCFastQC Report
Wed 25 May 2016
SRR1295204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345494
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107193.1025140812865057No Hit
GGTATCAACGCAGAGTACTTTTTTT74152.1462022495325535No Hit
TATCAACGCAGAGTACTTTTTTTTT66351.9204385604380974No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34861.008990025875992No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31650.916079584594812No Hit
ATACAGGGTGACAGCCCCGTACACA29110.8425616653255918No Hit
GTACTGGTTCACTATCGGTCAGTCA26740.7739642367161224No Hit
GGATACCACGTGTCCCGCCCTACTC22620.6547146983739226No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21020.6084041980468546No Hit
GTACATAAGCAGTGGTATCAACGCA20080.5811967791047022No Hit
ACCCTGTATCGCGCGCCTTTCCAGA19200.5557260039248149No Hit
GTGGTATCAACGCAGAGTACATGGG15250.44139695624236597No Hit
TCTAAGTACCCCGAGGAAAAGAAAT14300.4139000966731694No Hit
CCCATATTCAGACAGGATACCACGT14290.41361065604612524No Hit
CATCTAAGTACCCCGAGGAAAAGAA13950.40376967472662334No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13690.3962442184234748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13440.3890082027473704No Hit
GGTTAATGAGGCGAACCGGGGGAAC11950.3458815493177885No Hit
GTATTTAGCCTTGGAGGATGGTCCC11490.33256728047375644No Hit
GCTTATGTACTCTGCGTTGATACCA11110.32156853664607776No Hit
GCCTTGGAGGATGGTCCCCCCATAT10840.3137536397158851No Hit
ATCAACGCAGAGTACTTTTTTTTTT10740.3108592334454433No Hit
CCCCAGTAGCGGCGAGCGAACGGGG10720.310280352191355No Hit
CACACACACTGATTCAGGCTCTGGG10190.29493999895801376No Hit
GTTCACTATCGGTCAGTCAGGAGTA9960.2882828645359977No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9850.2850990176385118No Hit
CTATCGGTCAGTCAGGAGTATTTAG9670.27988908635171666No Hit
TATTCAGACAGGATACCACGTGTCC9480.27438971443787735No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG9300.2691797831510822No Hit
GTGGTATCAACGCAGAGTACTTTTT9290.26889034252403804No Hit
GTCAGGAGTATTTAGCCTTGGAGGA9070.26252264872906617No Hit
CTTATGTACTCTGCGTTGATACCAC8890.2573127174422711No Hit
CGGTACTGGTTCACTATCGGTCAGT8590.24862949863094583No Hit
CAGTAGCGGCGAGCGAACGGGGAGC8450.24457732985232739No Hit
TCACGGTACTGGTTCACTATCGGTC7900.22865809536489778No Hit
GGTATCAACGCAGAGTACATGGGGG7570.21910655467244003No Hit
GCCTGAATCAGTGTGTGTGTTAGTG7460.21592270777495412No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7080.20492396394727552No Hit
TTCCAGACGCTTCCACTAACACACA6940.20087179516865705No Hit
GTCCCGCCCTACTCATCGAGCTCAC6880.199135151406392No Hit
AGCTCACAGCATGTGCATTTTTGTG6850.19826682952525948No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC6810.19710906701708278No Hit
GTATCAACGCAGAGTACATGGGGGT6370.18437367942713911No Hit
GTATCGCGCGCCTTTCCAGACGCTT6360.18408423880009495No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6280.18176871378374154No Hit
GCCCTACTCATCGAGCTCACAGCAT6230.18032151064852067No Hit
GGGTGACAGCCCCGTACACAAAAAT6160.17829542625921146No Hit
GGTTCACTATCGGTCAGTCAGGAGT6110.17684822312399057No Hit
TCCCCCAGTAGCGGCGAGCGAACGG6060.1754010199887697No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6000.17366437622650466No Hit
CTACTGGGGGAATCTCGGTTGATTT5820.1684544449397095No Hit
GAATCAGTGTGTGTGTTAGTGGAAG5780.1672966824315328No Hit
GGAGTATTTAGCCTTGGAGGATGGT5630.1629550730258702No Hit
GAGTACATAAGCAGTGGTATCAACG5580.16150786989064933No Hit
CACTGATTCAGGCTCTGGGCTGCTC5430.15716626048498672No Hit
TAAGTACCCCGAGGAAAAGAAATCA5330.15427185421454495No Hit
CATGTACTCTGCGTTGATACCACTG5170.14964080418183817No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA5090.14732527916548477No Hit
GGTATCAACGCAGAGTACATGGGCA4980.14414143226799886No Hit
CTGTCACCCTGTATCGCGCGCCTTT4910.14211534787868965No Hit
GTACGGGGCTGTCACCCTGTATCGC4730.1369054165918945No Hit
GGGCTGTCACCCTGTATCGCGCGCC4660.13487933220258527No Hit
CCCATGTACTCTGCGTTGATACCAC4620.1337215696944086No Hit
GTCCCCCCATATTCAGACAGGATAC4510.13053772279692266No Hit
GTACATGGGGGTTAATGAGGCGAAC4490.12995884154283432No Hit
AACGCAGAGTACTTTTTTTTTTTTT4490.12995884154283432No Hit
AAGTACCCCGAGGAAAAGAAATCAA4470.129379960288746No Hit
GTTAATGATAGTGTGTCGAAACACA4440.12851163840761343No Hit
CAACGCAGAGTACTTTTTTTTTTTT4390.12706443527239258No Hit
AACACACACACTGATTCAGGCTCTG4310.1247489102560392No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4290.12417002900195083No Hit
CTAACACACACACTGATTCAGGCTC4270.12359114774786249No Hit
GGTATCAACGCAGAGTACATGGGGT4250.12301226649377413No Hit
TTTGTGTACGGGGCTGTCACCCTGT4200.12156506335855326No Hit
CGTCTGGAAAGGCGCGCGATACAGG4190.1212756227315091No Hit
GTGCATTTTTGTGTACGGGGCTGTC4130.11953897896924404No Hit
CCACGTGTCCCGCCCTACTCATCGA4120.11924953834219985No Hit
GGTATCAACGCAGAGTACATGGGGA4120.11924953834219985No Hit
TATGTACTCTGCGTTGATACCACTG4060.11751289457993483No Hit
GTATCAACGCAGAGTACATGGGCAT4000.11577625081766976No Hit
ACCGAGATTCCCCCAGTAGCGGCGA3990.1154868101906256No Hit
CCGCTACTGGGGGAATCTCGGTTGA3950.11432904768244889No Hit
CATAAGCAGTGGTATCAACGCAGAG3850.11143464141200715No Hit
ATCATGTACTCTGCGTTGATACCAC3840.11114520078496298No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3830.1108557601579188No Hit
GTACATGATAAGCAGTGGTATCAAC3810.11027687890383046No Hit
CTACTCATCGAGCTCACAGCATGTG3780.10940855702269794No Hit
GCGTTGATACCACTGCTTATGTACT3690.10680359137930037No Hit
GTCTGGAAAGGCGCGCGATACAGGG3680.10651415075225619No Hit
TATCAACGCAGAGTACATGGGGGTT3660.10593526949816785No Hit
GCTGTCACCCTGTATCGCGCGCCTT3650.10564582887112367No Hit
GTATGTGTGTTAGTGGAAGCGTCTG3620.10477750698999115No Hit
GTACACAAAAATGCACATGCTGTGA3610.10448806636294697No Hit
GGCTCTGGGCTGCTCCCCGTTCGCT3600.10419862573590279No Hit
GCATGTGCATTTTTGTGTACGGGGC3550.10275142260068192No Hit
GGAGGATGGTCCCCCCATATTCAGA3510.10159366009250521No Hit
GTCACCCTGTATCGCGCGCCTTTCC3510.10159366009250521No Hit
GTACATGGGCATCTAAGTACCCCGA3480.1007253382113727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA250.00602146819.0007251
AACCCCG250.00602146819.00072513
AGGACCG250.00602146819.0007255
GCACGCC250.00602146819.00072511
TATTCTG401.280067E-519.0007255
ATTCTGT359.908291E-519.0007256
TCTATTA250.00602146819.0007251
TTCTGTT307.7041483E-419.0007237
ACCCCGT250.00602650618.99797214
GCTCCGG555.8745536E-717.2733866
TTAAGCT504.5399665E-617.1006534
TAAGCTG504.5399665E-617.1006535
TGGCTCC504.5399665E-617.1006534
TTATTCT453.5248944E-516.8895324
TGATCGA350.002165056316.28633510
CAGAACG350.002165056316.2863359
AGAACGA350.002165056316.28633510
ATCCGGG350.002165056316.28633519
TCTGTTA350.002165056316.2863358
GTGTAAG350.002165056316.2863351
Produced by FastQC (version 0.11.5)