##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295204_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345494
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.807469883702755	31.0	30.0	33.0	28.0	34.0
2	30.670989944832616	31.0	30.0	33.0	27.0	34.0
3	30.914635275865862	31.0	31.0	34.0	27.0	34.0
4	34.468986436812216	35.0	35.0	37.0	31.0	37.0
5	34.826355884617385	37.0	35.0	37.0	32.0	37.0
6	34.559867899297814	37.0	35.0	37.0	31.0	37.0
7	34.41739364504159	37.0	35.0	37.0	30.0	37.0
8	34.47463921225839	37.0	35.0	37.0	30.0	37.0
9	35.88494445634367	39.0	37.0	39.0	30.0	39.0
10	35.946815284780634	39.0	37.0	39.0	30.0	39.0
11	36.01629550730259	39.0	37.0	39.0	30.0	39.0
12	35.962089066669755	39.0	37.0	39.0	30.0	39.0
13	35.78242458624462	39.0	35.0	39.0	30.0	39.0
14	36.743723480002544	40.0	36.0	41.0	29.0	41.0
15	36.74716782346437	40.0	36.0	41.0	29.0	41.0
16	36.699155412250285	40.0	36.0	41.0	29.0	41.0
17	36.83371635976312	40.0	37.0	41.0	30.0	41.0
18	36.89461177328695	40.0	37.0	41.0	30.0	41.0
19	36.98144685580647	40.0	37.0	41.0	30.0	41.0
20	37.01252699033847	40.0	37.0	41.0	30.0	41.0
21	36.966887992266145	40.0	37.0	41.0	30.0	41.0
22	36.93946638725998	40.0	37.0	41.0	30.0	41.0
23	36.87928589208496	40.0	36.0	41.0	29.0	41.0
24	36.866246591836614	40.0	36.0	41.0	29.0	41.0
25	35.568907130080404	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07267101614737825
1101	2	0.04904832095754941
1101	3	0.02078323195667764
1101	4	-0.27425502492661735
1101	5	-0.24092905608654291
1101	6	-0.008069207951308499
1101	7	-0.06731195133835399
1101	8	-0.17200610207607525
1101	9	-0.03993925469919901
1101	10	-0.10008342487035549
1101	11	0.08236502174272431
1101	12	0.11234446989362112
1101	13	0.36569102730263836
1101	14	-0.04674406059041303
1101	15	0.11564185834169649
1101	16	0.16842874421333676
1101	17	0.20204046582781388
1101	18	-0.07363703478991113
1101	19	0.029566401345860527
1101	20	-0.04715175007568462
1101	21	-0.10960850323384363
1101	22	0.08875172717566926
1101	23	0.025865995805148145
1101	24	-0.029106703970356307
1101	25	0.19082877752999394
1102	1	-0.4277264676812962
1102	2	-0.43808260227448415
1102	3	-0.2874192741645949
1102	4	-0.1948729134474263
1102	5	-0.4226660573828127
1102	6	-0.37816138712457814
1102	7	-0.4615939619965701
1102	8	-0.2958718661550037
1102	9	-0.2905225760300354
1102	10	-0.16348541248378012
1102	11	-0.0915223468299402
1102	12	-0.15052460687434888
1102	13	-0.0659883620103372
1102	14	0.12710730049268903
1102	15	-0.16178864720345132
1102	16	-0.12184203210814104
1102	17	-0.3923423658011629
1102	18	-0.10168686915546488
1102	19	-0.3687844556323938
1102	20	-0.3996146623614365
1102	21	-0.3965161800306163
1102	22	-0.5811676160477219
1102	23	-0.4410215167974698
1102	24	-0.4610381457102335
1102	25	-0.01549413763635954
1103	1	-0.27698783962805607
1103	2	-0.3684467970967731
1103	3	-0.1340040335619932
1103	4	-0.16139331713325333
1103	5	-0.3583176589624557
1103	6	-0.14915743802409764
1103	7	-0.09710661374440832
1103	8	0.09582039653330554
1103	9	-0.12441170869055895
1103	10	0.12498906462606385
1103	11	0.06727537146755935
1103	12	-0.11938521825361192
1103	13	0.15261628915244785
1103	14	-0.11041646724169141
1103	15	0.15579216309345867
1103	16	-0.1930867300750876
1103	17	0.2815570052680201
1103	18	-0.2451708533590633
1103	19	-0.06700736041518951
1103	20	-0.0030810950073387744
1103	21	-0.22249979857676294
1103	22	-0.550635218797467
1103	23	-0.1228410282791188
1103	24	-0.2838259181250393
1103	25	-0.45684268231970293
1104	1	-0.24782063162493984
1104	2	-0.22665995373789016
1104	3	-0.5799353487930361
1104	4	-0.46421692806391945
1104	5	-0.3288148223594476
1104	6	-0.2833439176048813
1104	7	-0.520323451473395
1104	8	-0.21217839087304213
1104	9	-0.30701532048736624
1104	10	-0.3234973586557359
1104	11	-0.28327246102128356
1104	12	-0.16827872654688747
1104	13	-0.18498277541816321
1104	14	0.017761177728203847
1104	15	0.015239566843838759
1104	16	-0.28314346213519315
1104	17	-0.27663666326084524
1104	18	-0.3260465192028974
1104	19	-0.25027140268274195
1104	20	-0.5491596537531649
1104	21	-0.4000473279171928
1104	22	-0.08093923334859454
1104	23	-0.10971903269642524
1104	24	-0.12718480253887776
1104	25	-0.18426895229501383
1105	1	-0.013596803899201149
1105	2	-0.20766694047848233
1105	3	0.1258024594146363
1105	4	0.18958587503320956
1105	5	0.04210199482317023
1105	6	0.034790804675679965
1105	7	0.26797760843895446
1105	8	-0.012604736065632949
1105	9	0.27319568187888876
1105	10	0.21544776952784872
1105	11	0.06692565997953892
1105	12	0.15289431779581264
1105	13	0.11559058535807765
1105	14	0.21050210826684435
1105	15	0.09549607403359772
1105	16	0.024710670402853907
1105	17	0.03844923584446036
1105	18	-0.1365854363281116
1105	19	0.11007779433214182
1105	20	-0.32109997942768587
1105	21	-0.11477408895386532
1105	22	-0.14071506958723035
1105	23	0.19453671841319675
1105	24	0.14088411276614465
1105	25	-0.14902371630000033
1106	1	-0.41282042302848865
1106	2	-0.3155410044571667
1106	3	-0.5262426227408561
1106	4	-0.29645228554134917
1106	5	-0.042836675682011105
1106	6	-0.2966258224318068
1106	7	-0.181706938978472
1106	8	-0.08632646401074595
1106	9	-0.3995107787737595
1106	10	-0.250151054415916
1106	11	-0.30687799375765223
1106	12	-0.48487883198291115
1106	13	-0.9065178935785667
1106	14	-0.579894198181556
1106	15	-0.5061301912222049
1106	16	-0.6978863006398228
1106	17	-0.4066643117720332
1106	18	-0.3001843521512768
1106	19	-0.14621211577918558
1106	20	-0.4383342565665629
1106	21	-0.5091281002550332
1106	22	-0.5338248807750787
1106	23	-0.3041195454644239
1106	24	-0.3161125030095491
1106	25	-0.330696074384619
1107	1	-0.06401202104654757
1107	2	0.09875209217778291
1107	3	0.021352902686995634
1107	4	0.046911091058341015
1107	5	0.10488956075292322
1107	6	0.22085297502692924
1107	7	0.3544437738524877
1107	8	0.127811603290219
1107	9	-0.05788388270421052
1107	10	0.30065145037175256
1107	11	0.21796089732664115
1107	12	0.47122278822562436
1107	13	0.4802802947060769
1107	14	0.2485590042381176
1107	15	0.17146958991285288
1107	16	0.333191761189525
1107	17	0.2485968062630448
1107	18	0.31305545694986137
1107	19	0.09066358717245038
1107	20	0.2017089084636794
1107	21	0.08232780757850833
1107	22	0.3646575036368702
1107	23	0.026051138792503536
1107	24	-0.06986664472459836
1107	25	0.11862301246220142
1108	1	0.23784374437413902
1108	2	0.2285896301516317
1108	3	0.058765397733886005
1108	4	0.21187278700339363
1108	5	0.2587331745423782
1108	6	0.17331632200883007
1108	7	0.31240837562239676
1108	8	0.3248661535477808
1108	9	0.2611888950001884
1108	10	-0.12743598853988658
1108	11	-0.0824056328543179
1108	12	-0.19241807823851786
1108	13	-0.07041314077140015
1108	14	0.019860681691554305
1108	15	0.24939852928677908
1108	16	0.601884800418155
1108	17	0.4442413710497277
1108	18	0.22856859920319295
1108	19	0.010343946438069906
1108	20	0.033078608635385365
1108	21	0.04688004638448007
1108	22	0.03319204332779435
1108	23	-0.09846015429955912
1108	24	0.017539246174884227
1108	25	0.08156714137516019
1109	1	0.1946195200431866
1109	2	0.16659703270749304
1109	3	0.33093879422757055
1109	4	0.2493059583740873
1109	5	0.4156186177056753
1109	6	0.48777567649377573
1109	7	0.37897045199362367
1109	8	0.5222036704929351
1109	9	0.2755736146626546
1109	10	0.4494363554504872
1109	11	0.4635648483118189
1109	12	0.4169127977305962
1109	13	0.48530251578656447
1109	14	0.7593516417390802
1109	15	0.3774962552094294
1109	16	0.5470632385702601
1109	17	0.5545059438796045
1109	18	0.5972219737633822
1109	19	0.765889009116087
1109	20	0.42418944896256505
1109	21	0.5184807348526377
1109	22	0.5473680901192637
1109	23	0.4823571731645302
1109	24	0.4291122131375502
1109	25	0.15582726657888202
1110	1	0.12743660273472202
1110	2	0.3016953443930639
1110	3	0.27492087717532243
1110	4	0.13508776460827931
1110	5	0.16185299228179417
1110	6	0.11160499426523529
1110	7	-0.04003958866940138
1110	8	0.16439314392330573
1110	9	-0.09890905456818189
1110	10	0.18352909367885673
1110	11	0.24654063436822327
1110	12	-0.030407632351007408
1110	13	0.22875769753431996
1110	14	0.3171314223576758
1110	15	0.268941947912694
1110	16	0.01441676576384765
1110	17	0.10540405722365165
1110	18	0.0032101998727256387
1110	19	0.07162132039774605
1110	20	0.09036980712274811
1110	21	0.30257181521110255
1110	22	-0.060895336366264985
1110	23	0.13147955712117465
1110	24	0.39721781040779547
1110	25	0.22570799037474387
1111	1	-0.08195674970009748
1111	2	0.2129074637299162
1111	3	0.22496821833490444
1111	4	0.3061471545571024
1111	5	0.06959342658278445
1111	6	0.1473336458776231
1111	7	0.18041550530124795
1111	8	0.021382617477236465
1111	9	-0.009310495224646331
1111	10	0.22991414748688044
1111	11	0.317131563430749
1111	12	0.34689522111418114
1111	13	0.22871128463277302
1111	14	0.2182533768977848
1111	15	0.21941009937965106
1111	16	0.23926870860790928
1111	17	0.12401872493821742
1111	18	0.18066528765498902
1111	19	0.017407957195125334
1111	20	-0.11188214686040254
1111	21	0.06631467535974167
1111	22	0.05094119120609264
1111	23	0.23054325418726052
1111	24	0.021307343989079186
1111	25	-0.10680022470882733
1112	1	0.046416272269482306
1112	2	-0.11882091236472192
1112	3	-0.09130770730651605
1112	4	-0.08796561238098377
1112	5	-0.020451127112103507
1112	6	-0.07896532477839457
1112	7	0.0027046434177080414
1112	8	0.18796734631423107
1112	9	0.04027315817832999
1112	10	-0.04812856260683418
1112	11	-0.003482970291791787
1112	12	-0.18804716634932817
1112	13	-0.39033618809729376
1112	14	-0.4203086141914767
1112	15	-0.28927018945120153
1112	16	-0.06491012070534907
1112	17	0.038223671934687786
1112	18	-0.06209704142781902
1112	19	-0.14273031289243931
1112	20	-0.1399263932884267
1112	21	0.031227872471433216
1112	22	-0.10861634451367763
1112	23	0.25659948402287114
1112	24	0.20767391335456864
1112	25	-0.01436206216465763
1113	1	-0.02174426284757658
1113	2	-0.07728245082626017
1113	3	0.2666856037302736
1113	4	0.005947431097276024
1113	5	0.21065255181766673
1113	6	-0.3238482679222088
1113	7	-0.12298097531139263
1113	8	-0.19932362358543543
1113	9	-0.23277032008253684
1113	10	-0.347192107757337
1113	11	-0.09150972614463626
1113	12	-0.2571441563158814
1113	13	-0.25762715799696423
1113	14	-0.3353587814155574
1113	15	-0.4564942697187533
1113	16	-0.07193353207659214
1113	17	0.1296617990249871
1113	18	-0.09373583741443525
1113	19	-0.21216175101952928
1113	20	-0.29062873442555315
1113	21	-0.30930724458541903
1113	22	-0.2903554749484627
1113	23	-0.4544373052747872
1113	24	-0.23266053480596582
1113	25	-0.43904433641215235
1114	1	0.45411435216354334
1114	2	0.3964766672718554
1114	3	0.410441722547084
1114	4	0.31566566842531785
1114	5	0.40191332517952105
1114	6	0.3943635945183033
1114	7	0.22204106657668632
1114	8	0.14878541443753335
1114	9	0.25498072825116225
1114	10	0.3442469247763782
1114	11	0.19101125467694402
1114	12	0.03691273948455631
1114	13	0.36768338079846075
1114	14	0.4031887741701823
1114	15	0.3487052390781926
1114	16	0.5422259051903282
1114	17	0.19145548242537558
1114	18	0.5112333492144288
1114	19	0.17947082004362613
1114	20	0.482752926949928
1114	21	0.29391896347415525
1114	22	0.556367102848931
1114	23	0.3002101659915297
1114	24	0.07414047702705062
1114	25	0.2642842999334789
1115	1	0.08889558624571237
1115	2	0.0670163074336827
1115	3	0.088039366561393
1115	4	0.21476343831858458
1115	5	0.17795416850998436
1115	6	-0.14014480424281572
1115	7	-0.30386072441741163
1115	8	-0.5108291032118686
1115	9	-0.6234728926716784
1115	10	-0.38282993164549595
1115	11	-0.41840097841575385
1115	12	-0.32344358578667
1115	13	-0.45802988450169124
1115	14	-0.44419909109834776
1115	15	-0.4195545282506572
1115	16	-0.17688544792063965
1115	17	-0.3190773661276012
1115	18	-0.310311273412168
1115	19	-0.35301766056808503
1115	20	-0.3693802822608987
1115	21	-0.4998327563836895
1115	22	-0.533247004962
1115	23	-0.4500049992026405
1115	24	-0.29954438061263033
1115	25	-0.3180990863861126
1116	1	0.16274939247408682
1116	2	0.10248343546136596
1116	3	0.3097089167344045
1116	4	0.5932236459310403
1116	5	0.40232700588022396
1116	6	0.4450628082139332
1116	7	0.3489290679701327
1116	8	0.12487591408916643
1116	9	0.06110755715531724
1116	10	0.19340276731644224
1116	11	0.053809229196701835
1116	12	-0.213149468139612
1116	13	-0.10606189142210809
1116	14	-0.27443265511219295
1116	15	-0.2939057393233213
1116	16	-0.23257316418361285
1116	17	-0.33118809277119965
1116	18	-0.3883822076682222
1116	19	-0.28186714153694226
1116	20	-0.13787076668996434
1116	21	-0.19576701243904893
1116	22	-0.25718731638068704
1116	23	-0.5387809507597368
1116	24	-0.4195703321697266
1116	25	-2.3854650033411318
1201	1	-0.11088657342859065
1201	2	0.11447959668977248
1201	3	-0.03742470638854911
1201	4	-0.20042826780950662
1201	5	-0.2761300055231857
1201	6	-0.11061349301672863
1201	7	-0.25927983459281023
1201	8	-0.016872843955319183
1201	9	-0.025727008724778955
1201	10	0.23638783562399368
1201	11	0.1401004395388057
1201	12	0.08386137074488431
1201	13	0.0938964748418698
1201	14	0.2371895081485036
1201	15	0.2145268563637046
1201	16	0.2863207897511302
1201	17	0.4400662822993695
1201	18	0.360975644877783
1201	19	0.004735263340556628
1201	20	0.11388984638443844
1201	21	0.18315643793918213
1201	22	0.08496789983247766
1201	23	0.20576079588133922
1201	24	0.08118472033112312
1201	25	-0.15624306024527357
1202	1	-0.2241231196886737
1202	2	-0.03473460965141584
1202	3	-0.38547573607408836
1202	4	-0.44575247083145797
1202	5	-0.49361229089111447
1202	6	-0.49528438329424773
1202	7	-0.33164645171142126
1202	8	-0.3133566157648815
1202	9	-0.20988702488803312
1202	10	-0.48380035077290273
1202	11	-0.3766833625768484
1202	12	-0.3865793664147077
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2210	21	0.7320321036295638
2210	22	0.7607839821566174
2210	23	0.7507427522814112
2210	24	0.7549337717974396
2210	25	0.72145189132371
2211	1	0.20944971114506927
2211	2	0.17308734309902007
2211	3	0.20238601421638336
2211	4	0.14842947604283552
2211	5	0.25714559850377583
2211	6	0.22911097454974083
2211	7	0.203022814705065
2211	8	0.35602665591343197
2211	9	0.21537239364052851
2211	10	0.08478363138497969
2211	11	0.25991034193309304
2211	12	0.18857187759228822
2211	13	0.37255515486354795
2211	14	0.11652048458063291
2211	15	-0.047321004185924664
2211	16	-0.16990664553352275
2211	17	-0.16234471172428755
2211	18	0.028260515140260623
2211	19	0.24480849319296993
2211	20	0.03680076711005853
2211	21	-0.11653025556933727
2211	22	0.0010569893344936077
2211	23	-0.04949365058211441
2211	24	0.03127728040993816
2211	25	0.08553136589928556
2212	1	0.26987974461494346
2212	2	0.20792561233211515
2212	3	-0.05241093120477203
2212	4	0.1408927135580882
2212	5	0.2685609994776641
2212	6	0.0980740126957258
2212	7	0.21838935208378274
2212	8	0.10080378523763045
2212	9	0.08710545631497979
2212	10	0.39294508424320895
2212	11	0.2223141311574821
2212	12	0.1830590152537468
2212	13	0.23911367880598533
2212	14	0.14005814120633886
2212	15	0.08377033110485144
2212	16	0.13158499908169574
2212	17	-0.05517768860953254
2212	18	-0.07007988673985466
2212	19	0.08000260636221412
2212	20	0.11114133815197391
2212	21	-0.12148730055429269
2212	22	0.02308729346324867
2212	23	0.03243503563481909
2212	24	0.03973839131966628
2212	25	0.21903929837955616
2213	1	0.10512742603046377
2213	2	0.24212689895881923
2213	3	0.019205321408328757
2213	4	0.19085921968515862
2213	5	0.31599236591218016
2213	6	0.20396373700650372
2213	7	0.011914062477274001
2213	8	-0.03085600941028588
2213	9	-0.06727242386935472
2213	10	-0.055714005878407136
2213	11	-0.06150024348880834
2213	12	-0.012397945119538178
2213	13	-0.0478219783091518
2213	14	0.050363859418915524
2213	15	-0.01097979305347252
2213	16	-0.11080958184065537
2213	17	-0.14133490191930775
2213	18	-0.10541881008508369
2213	19	-0.04066195827518726
2213	20	0.19411952093612683
2213	21	-0.07875662594149446
2213	22	0.2642691651932694
2213	23	0.092002156201211
2213	24	-0.073826049736887
2213	25	0.1345683283277026
2214	1	0.2491895589929527
2214	2	0.3625336867176472
2214	3	0.43776956310350457
2214	4	0.1993055259192147
2214	5	0.2307614575503436
2214	6	0.39509483107332954
2214	7	0.2610601892628708
2214	8	0.4962333138329882
2214	9	0.49354240295293295
2214	10	0.2818197295787357
2214	11	0.32532920653437003
2214	12	0.23311371049867802
2214	13	0.44171683061947675
2214	14	0.39555330409561407
2214	15	0.36639946315968075
2214	16	0.32767599829544736
2214	17	0.3389271895226216
2214	18	0.29452374365703093
2214	19	0.47758576022678056
2214	20	0.4040514670276423
2214	21	0.4053390078585153
2214	22	0.3991427407678003
2214	23	0.06889283483490516
2214	24	0.3429124247521358
2214	25	0.11387914386548914
2215	1	0.3293800153114539
2215	2	0.20196440431949725
2215	3	0.2748905776340571
2215	4	0.26147624108674705
2215	5	0.15027250761033173
2215	6	-0.39966037517707065
2215	7	-0.16891262753159708
2215	8	-0.05581180217380677
2215	9	-0.16845559163361656
2215	10	-0.22193027766626017
2215	11	-0.27480236249880363
2215	12	-0.3563155581050097
2215	13	-0.4026665627023789
2215	14	-0.16911258590803868
2215	15	-0.10813584312955271
2215	16	-0.44678164169226164
2215	17	-0.2792849785843501
2215	18	-0.11999985472704822
2215	19	-0.2855436121251813
2215	20	-0.2603837424685125
2215	21	0.11781430243984659
2215	22	0.1363031680483786
2215	23	0.01539292467278841
2215	24	0.05166669205172525
2215	25	-0.04474268500202783
2216	1	0.03436770196004346
2216	2	-0.10157061859268879
2216	3	0.1025348308838474
2216	4	0.08107473231153506
2216	5	0.26329348706729405
2216	6	-0.34008557546385987
2216	7	-0.28564631623760306
2216	8	-0.3945630101291684
2216	9	-0.5477722785627606
2216	10	-0.4474391775696205
2216	11	-0.34255073370738387
2216	12	-0.26361316713272487
2216	13	-0.44346583948781415
2216	14	-0.9060900477989975
2216	15	-0.4537825278765766
2216	16	-0.29828262364307534
2216	17	-0.3711456974346845
2216	18	-0.6594805118547669
2216	19	-0.508543082183472
2216	20	-0.3966909839660602
2216	21	-0.22158842208398966
2216	22	-0.5622316989986018
2216	23	-0.9455940138227987
2216	24	-0.45332099459685793
2216	25	-0.6334611612637602
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	54.0
9	135.0
10	254.0
11	401.0
12	519.0
13	690.0
14	1016.0
15	1314.0
16	1810.0
17	2149.0
18	2269.0
19	2367.0
20	2228.0
21	2164.0
22	2183.0
23	1993.0
24	2181.0
25	2304.0
26	2276.0
27	2493.0
28	3034.0
29	3556.0
30	4609.0
31	6054.0
32	8257.0
33	11346.0
34	16146.0
35	23742.0
36	36986.0
37	65554.0
38	135404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16128397234735	18.67827738341999	13.745266741549614	21.415171902683046
2	16.365983484374414	22.398846858528877	37.52456648653656	23.71060317056016
3	18.873953666076204	29.37086821113079	28.59024232109571	23.16493580169729
4	12.18805536420314	18.93607414311102	34.11028845652891	34.76558203615692
5	13.00278767632849	37.62813034630293	31.410425272616216	17.958656704752364
6	29.245820971126708	36.97778421014545	16.994572689774948	16.781822128952893
7	25.63850971947252	34.342329200097254	20.464439118706075	19.55472196172415
8	26.772854628814898	32.282452646691056	20.83458759783263	20.110105126661416
9	27.06232415972954	15.753262090284933	20.45796621551215	36.72644753447338
10	19.925902341601784	28.276650554285215	25.82187617586616	25.97557092824684
11	31.531036079714024	26.29665542642951	20.12243657467039	22.04987191918607
12	25.578110391659116	27.706170273735886	25.315576344727177	21.40014298987782
13	31.20151223917927	22.40713508255946	26.084967925708064	20.306384752553207
14	24.552714683926098	25.13857812332657	26.48513776764031	23.823569425107028
15	28.500060782531676	25.39725726061813	25.33821137270112	20.764470584149073
16	20.97634112314541	28.76721448129345	25.512165189554665	24.74427920600647
17	22.07274221838874	26.78628282980312	25.792633157160473	25.348341794647666
18	20.592083174140065	23.058131592048444	33.10670001041992	23.243085223391567
19	22.979336773827544	25.734240635845012	32.1968896620192	19.089532928308245
20	22.687776780804075	22.134938782598628	34.03513849894353	21.142145937653765
21	24.59412600690615	23.976740214594386	31.029352598780278	20.39978117971918
22	23.704151808456444	23.6141342101607	31.149272912517944	21.53244106886491
23	23.8059677732142	22.876560469363714	31.735597949572924	21.581873807849163
24	23.575654429046114	23.139175436768433	32.23345258356199	21.051717550623458
25	22.986659256750695	24.746224850426792	31.44148916155064	20.82562673127187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1735.0
1	1735.0
2	1388.0
3	1041.0
4	1041.0
5	1041.0
6	2559.0
7	4077.0
8	4077.0
9	4077.0
10	3692.0
11	3307.0
12	3307.0
13	3307.0
14	2493.0
15	1679.0
16	1679.0
17	1679.0
18	2968.0
19	4257.0
20	4257.0
21	4257.0
22	6437.5
23	8618.0
24	8618.0
25	8618.0
26	12328.5
27	16039.0
28	16039.0
29	16039.0
30	18584.0
31	21129.0
32	21129.0
33	21129.0
34	21172.0
35	21215.0
36	21215.0
37	21215.0
38	21157.5
39	21100.0
40	21100.0
41	21100.0
42	30346.5
43	39593.0
44	39593.0
45	39593.0
46	45404.5
47	51216.0
48	51216.0
49	51216.0
50	48990.5
51	46765.0
52	46765.0
53	46765.0
54	40427.0
55	34089.0
56	34089.0
57	34089.0
58	29506.0
59	24923.0
60	24923.0
61	24923.0
62	23675.0
63	22427.0
64	22427.0
65	22427.0
66	15731.0
67	9035.0
68	9035.0
69	9035.0
70	8623.0
71	8211.0
72	8211.0
73	8211.0
74	5854.0
75	3497.0
76	3497.0
77	3497.0
78	2318.5
79	1140.0
80	1140.0
81	1140.0
82	733.5
83	327.0
84	327.0
85	327.0
86	193.0
87	59.0
88	59.0
89	59.0
90	37.0
91	15.0
92	15.0
93	15.0
94	7.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019103081384915514
2	8.683218811325233E-4
3	5.788812540883489E-4
4	0.0
5	0.013024828216987848
6	0.005499371913839314
7	5.788812540883489E-4
8	0.0017366437622650466
9	0.0028944062704417445
10	0.0011577625081766979
11	0.00260496564339757
12	0.004341609405662616
13	0.013314268844032024
14	0.006078253167927663
15	0.0
16	0.0
17	0.0
18	5.788812540883489E-4
19	2.8944062704417447E-4
20	0.0011577625081766979
21	0.0014472031352208722
22	0.0017366437622650466
23	0.0014472031352208722
24	5.788812540883489E-4
25	0.0031838468974859186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	345494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.761628277191498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.51416042389914	19.606708075972374
2	6.688592484316949	3.1786369661991234
3	2.2486144101346	1.6029221925706378
4	1.2448992021438576	1.183233283356585
5	0.8819051099336135	1.0477750698999115
6	0.6516840246056398	0.9291044128117999
7	0.5664169559656496	0.9421292410287878
8	0.4446068579085206	0.8451666309689894
9	0.36299409221024426	0.7762797617324758
>10	3.4045922406967537	16.995664179406877
>50	0.5189110177233692	8.506660028828286
>100	0.4019733235885255	19.54100505363335
>500	0.04141543333942384	7.046142624763382
>1k	0.025580120591997074	10.629417587570261
>5k	0.0024362019611425785	4.066640809970651
>10k+	0.0012181009805712893	3.1025140812865057
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10719	3.1025140812865057	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7415	2.1462022495325535	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6635	1.9204385604380974	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3486	1.008990025875992	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3165	0.916079584594812	No Hit
ATACAGGGTGACAGCCCCGTACACA	2911	0.8425616653255918	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2674	0.7739642367161224	No Hit
GGATACCACGTGTCCCGCCCTACTC	2262	0.6547146983739226	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2102	0.6084041980468546	No Hit
GTACATAAGCAGTGGTATCAACGCA	2008	0.5811967791047022	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1920	0.5557260039248149	No Hit
GTGGTATCAACGCAGAGTACATGGG	1525	0.44139695624236597	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1430	0.4139000966731694	No Hit
CCCATATTCAGACAGGATACCACGT	1429	0.41361065604612524	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	1395	0.40376967472662334	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1369	0.3962442184234748	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1344	0.3890082027473704	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1195	0.3458815493177885	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1149	0.33256728047375644	No Hit
GCTTATGTACTCTGCGTTGATACCA	1111	0.32156853664607776	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1084	0.3137536397158851	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1074	0.3108592334454433	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	1072	0.310280352191355	No Hit
CACACACACTGATTCAGGCTCTGGG	1019	0.29493999895801376	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	996	0.2882828645359977	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	985	0.2850990176385118	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	967	0.27988908635171666	No Hit
TATTCAGACAGGATACCACGTGTCC	948	0.27438971443787735	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	930	0.2691797831510822	No Hit
GTGGTATCAACGCAGAGTACTTTTT	929	0.26889034252403804	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	907	0.26252264872906617	No Hit
CTTATGTACTCTGCGTTGATACCAC	889	0.2573127174422711	No Hit
CGGTACTGGTTCACTATCGGTCAGT	859	0.24862949863094583	No Hit
CAGTAGCGGCGAGCGAACGGGGAGC	845	0.24457732985232739	No Hit
TCACGGTACTGGTTCACTATCGGTC	790	0.22865809536489778	No Hit
GGTATCAACGCAGAGTACATGGGGG	757	0.21910655467244003	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	746	0.21592270777495412	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG	708	0.20492396394727552	No Hit
TTCCAGACGCTTCCACTAACACACA	694	0.20087179516865705	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	688	0.199135151406392	No Hit
AGCTCACAGCATGTGCATTTTTGTG	685	0.19826682952525948	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	681	0.19710906701708278	No Hit
GTATCAACGCAGAGTACATGGGGGT	637	0.18437367942713911	No Hit
GTATCGCGCGCCTTTCCAGACGCTT	636	0.18408423880009495	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	628	0.18176871378374154	No Hit
GCCCTACTCATCGAGCTCACAGCAT	623	0.18032151064852067	No Hit
GGGTGACAGCCCCGTACACAAAAAT	616	0.17829542625921146	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	611	0.17684822312399057	No Hit
TCCCCCAGTAGCGGCGAGCGAACGG	606	0.1754010199887697	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA	600	0.17366437622650466	No Hit
CTACTGGGGGAATCTCGGTTGATTT	582	0.1684544449397095	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	578	0.1672966824315328	No Hit
GGAGTATTTAGCCTTGGAGGATGGT	563	0.1629550730258702	No Hit
GAGTACATAAGCAGTGGTATCAACG	558	0.16150786989064933	No Hit
CACTGATTCAGGCTCTGGGCTGCTC	543	0.15716626048498672	No Hit
TAAGTACCCCGAGGAAAAGAAATCA	533	0.15427185421454495	No Hit
CATGTACTCTGCGTTGATACCACTG	517	0.14964080418183817	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA	509	0.14732527916548477	No Hit
GGTATCAACGCAGAGTACATGGGCA	498	0.14414143226799886	No Hit
CTGTCACCCTGTATCGCGCGCCTTT	491	0.14211534787868965	No Hit
GTACGGGGCTGTCACCCTGTATCGC	473	0.1369054165918945	No Hit
GGGCTGTCACCCTGTATCGCGCGCC	466	0.13487933220258527	No Hit
CCCATGTACTCTGCGTTGATACCAC	462	0.1337215696944086	No Hit
GTCCCCCCATATTCAGACAGGATAC	451	0.13053772279692266	No Hit
GTACATGGGGGTTAATGAGGCGAAC	449	0.12995884154283432	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	449	0.12995884154283432	No Hit
AAGTACCCCGAGGAAAAGAAATCAA	447	0.129379960288746	No Hit
GTTAATGATAGTGTGTCGAAACACA	444	0.12851163840761343	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	439	0.12706443527239258	No Hit
AACACACACACTGATTCAGGCTCTG	431	0.1247489102560392	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	429	0.12417002900195083	No Hit
CTAACACACACACTGATTCAGGCTC	427	0.12359114774786249	No Hit
GGTATCAACGCAGAGTACATGGGGT	425	0.12301226649377413	No Hit
TTTGTGTACGGGGCTGTCACCCTGT	420	0.12156506335855326	No Hit
CGTCTGGAAAGGCGCGCGATACAGG	419	0.1212756227315091	No Hit
GTGCATTTTTGTGTACGGGGCTGTC	413	0.11953897896924404	No Hit
CCACGTGTCCCGCCCTACTCATCGA	412	0.11924953834219985	No Hit
GGTATCAACGCAGAGTACATGGGGA	412	0.11924953834219985	No Hit
TATGTACTCTGCGTTGATACCACTG	406	0.11751289457993483	No Hit
GTATCAACGCAGAGTACATGGGCAT	400	0.11577625081766976	No Hit
ACCGAGATTCCCCCAGTAGCGGCGA	399	0.1154868101906256	No Hit
CCGCTACTGGGGGAATCTCGGTTGA	395	0.11432904768244889	No Hit
CATAAGCAGTGGTATCAACGCAGAG	385	0.11143464141200715	No Hit
ATCATGTACTCTGCGTTGATACCAC	384	0.11114520078496298	No Hit
AGTCAGGAGTATTTAGCCTTGGAGG	383	0.1108557601579188	No Hit
GTACATGATAAGCAGTGGTATCAAC	381	0.11027687890383046	No Hit
CTACTCATCGAGCTCACAGCATGTG	378	0.10940855702269794	No Hit
GCGTTGATACCACTGCTTATGTACT	369	0.10680359137930037	No Hit
GTCTGGAAAGGCGCGCGATACAGGG	368	0.10651415075225619	No Hit
TATCAACGCAGAGTACATGGGGGTT	366	0.10593526949816785	No Hit
GCTGTCACCCTGTATCGCGCGCCTT	365	0.10564582887112367	No Hit
GTATGTGTGTTAGTGGAAGCGTCTG	362	0.10477750698999115	No Hit
GTACACAAAAATGCACATGCTGTGA	361	0.10448806636294697	No Hit
GGCTCTGGGCTGCTCCCCGTTCGCT	360	0.10419862573590279	No Hit
GCATGTGCATTTTTGTGTACGGGGC	355	0.10275142260068192	No Hit
GGAGGATGGTCCCCCCATATTCAGA	351	0.10159366009250521	No Hit
GTCACCCTGTATCGCGCGCCTTTCC	351	0.10159366009250521	No Hit
GTACATGGGCATCTAAGTACCCCGA	348	0.1007253382113727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.894406270441744E-4	0.0	0.0	0.0	0.0
9	2.894406270441744E-4	0.0	0.0	0.0	0.0
10	2.894406270441744E-4	0.0	0.0	0.0	0.0
11	5.788812540883488E-4	0.0	0.0	0.0	0.0
12	5.788812540883488E-4	0.0	0.0	2.894406270441744E-4	0.0
13	5.788812540883488E-4	0.0	0.0	2.894406270441744E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	25	0.006021468	19.000725	1
AACCCCG	25	0.006021468	19.000725	13
AGGACCG	25	0.006021468	19.000725	5
GCACGCC	25	0.006021468	19.000725	11
TATTCTG	40	1.280067E-5	19.000725	5
ATTCTGT	35	9.908291E-5	19.000725	6
TCTATTA	25	0.006021468	19.000725	1
TTCTGTT	30	7.7041483E-4	19.000723	7
ACCCCGT	25	0.006026506	18.997972	14
GCTCCGG	55	5.8745536E-7	17.273386	6
TTAAGCT	50	4.5399665E-6	17.100653	4
TAAGCTG	50	4.5399665E-6	17.100653	5
TGGCTCC	50	4.5399665E-6	17.100653	4
TTATTCT	45	3.5248944E-5	16.889532	4
TGATCGA	35	0.0021650563	16.286335	10
CAGAACG	35	0.0021650563	16.286335	9
AGAACGA	35	0.0021650563	16.286335	10
ATCCGGG	35	0.0021650563	16.286335	19
TCTGTTA	35	0.0021650563	16.286335	8
GTGTAAG	35	0.0021650563	16.286335	1
>>END_MODULE