FastQCFastQC Report
Wed 25 May 2016
SRR1295203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590467
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG56380.12281974796899749No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54540.11881144118888122No Hit
GTATCAACGCAGAGTACTTTTTTTT49980.10887781134250611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG701.0864872E-412.2206645
GGTATCA33850.010.8964531
GTGGTAT14900.010.8461281
TGGTATC15450.09.8432182
GTGTTAG5950.09.5861981
GTCGTAA800.0044935919.5063121
TACATCG1106.803531E-59.5049615
GGACCGT1252.7617434E-59.1185786
TATACTG8450.08.8862945
TAGAACG1554.180243E-68.5854074
ACACCGT1554.2204956E-68.5793076
GACTCGC1000.00291045278.5487611
ACTAGAC2901.8189894E-128.5217833
GGACAGT6400.08.4596196
CTAGCAC4300.08.3998253
CCTATAC3750.08.3644573
CCGGTCG1252.7496283E-48.3587889
GCTAGAC3001.8189894E-128.2377223
CTAGACT3950.08.1817544
GTTAGAC2105.7640136E-88.1471993
Produced by FastQC (version 0.11.5)