FastQCFastQC Report
Wed 25 May 2016
SRR1295203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590467
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144130.31397676968378163No Hit
GGTATCAACGCAGAGTACTTTTTTT123320.2686436913717058No Hit
TATCAACGCAGAGTACTTTTTTTTT86660.1887825356330848No Hit
GTGGTATCAACGCAGAGTACATGGG63720.1388094065375048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57800.12591311515800027No Hit
ATTCCATTCCATTCCATTCCATTCC46150.10053443364259017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.012.1881971
AGCGTCA4650.012.0537833
TGGTATC15700.011.6781092
GTGGTAT17500.011.1306521
GCGGTAT909.505423E-510.55752851
GCAGCGT5050.010.5366231
ATACCGA856.5980694E-410.0588416
CAGCGTC5750.09.5824172
ACCCGTG1304.2398187E-69.5006377
GCTTACG800.0045190359.4998092
GTCTACG1054.4873098E-49.0493111
GACCCGT850.0074392718.9411926
ACTAGAC3300.08.9242583
TCTATAC6050.08.4793533
CGCAGTA1356.8053545E-58.4442752
GTCTAGA3950.08.4192941
AGTACTG5700.08.3338925
GTGTAGG5150.08.3025231
CTACACT5750.08.2608834
TTAGACT3950.08.177234
Produced by FastQC (version 0.11.5)