FastQCFastQC Report
Wed 25 May 2016
SRR1295202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4774396
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG57330.12007801615115295No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51500.10786704747574354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.011.0544451
AACCGAT700.001494339410.8554367
GTGGTAT13200.09.6502371
GTCTTAG4650.08.9951171
TAGCACG850.00740675958.9459794
CGTAGAC1401.0794691E-58.8257963
TGGTATC14150.08.7322362
AGGACCG2201.5934347E-98.6406395
CTAGTAC3200.08.6136373
GTCTTAT4600.08.472921
TCTAGAC4050.08.4486263
GTATTAG5550.08.3928651
ATACCGT1502.5939204E-58.2319526
GTATCAA52500.08.1843891
CCGTAGA1050.0044952998.1468892
CTTATAC4900.08.1468893
ACCGATC1401.04161896E-48.1417488
AGACAGT8750.08.1414916
GTATAAA7750.08.0956061
TAGACAC4700.08.0891085
Produced by FastQC (version 0.11.5)