FastQCFastQC Report
Wed 25 May 2016
SRR1295202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4774396
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133690.2800144772239253No Hit
GGTATCAACGCAGAGTACTTTTTTT111840.2342495260133428No Hit
TATCAACGCAGAGTACTTTTTTTTT79520.1665550993256529No Hit
GTGGTATCAACGCAGAGTACATGGG68710.1439134918846279No Hit
GGTATCAACGCAGAGTACATGGGGG55480.11620318046513109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.012.2744991
CCGTGCG855.3242504E-511.177249
GTGGTAT16450.011.089841
TACGCTG600.005877042711.0837495
GATCGAC1002.399406E-510.45072111
TGGTATC16800.010.178742
AGCGTCA4650.09.8067163
GGATCGT800.0045188969.4998596
GTGCTAG3450.09.0883451
CTTAGAC2950.09.0171933
TCTATAC5300.08.9625063
GCGTTAT850.00743526558.941792511
TTAGGAC3150.08.7462673
GCAGCGT5050.08.6547871
TACACTG9950.08.5932875
ACCGCGT1000.00290487058.550598
GTATTAG5450.08.5425881
GTCCTAG4500.08.4457341
CCGTGCA2252.4483597E-98.4450269
TAAAGCG1252.744283E-48.3603135
Produced by FastQC (version 0.11.5)