FastQCFastQC Report
Wed 25 May 2016
SRR1295201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059854
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA292482.759625382363986No Hit
GTACATAAGCAGTGGTATCAACGCA155331.46557922128897No Hit
GCTTATGTACTCTGCGTTGATACCA92440.872195604300215No Hit
GTATCAACGCAGAGTACTTTTTTTT80610.7605764567572515No Hit
CTTATGTACTCTGCGTTGATACCAC64430.6079139202192No Hit
GGTATCAACGCAGAGTACTTTTTTT57380.5413953242616436No Hit
TATCAACGCAGAGTACTTTTTTTTT53370.503559924291459No Hit
GTGGTATCAACGCAGAGTACATGGG45800.4321349921781679No Hit
CCCATGTACTCTGCGTTGATACCAC43400.40949036376708486No Hit
GAGTACATAAGCAGTGGTATCAACG43370.4092073059119464No Hit
TATGTACTCTGCGTTGATACCACTG34810.3284414645790835No Hit
GCGTTGATACCACTGCTTATGTACT32720.30872176733776535No Hit
CATAAGCAGTGGTATCAACGCAGAG28810.27182989355137593No Hit
CTGCTTATGTACTCTGCGTTGATAC26150.24673209706242558No Hit
CATGTACTCTGCGTTGATACCACTG26100.24626033397052802No Hit
ACGCAGAGTACATAAGCAGTGGTAT25300.238712124500167No Hit
ACTCTGCGTTGATACCACTGCTTAT24250.22880509957031817No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24130.22767286814976403No Hit
GTACTGGTTCACTATCGGTCAGTCA23460.22135124271833667No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22790.21502961728690934No Hit
ATACAGGGTGACAGCCCCGTACACA22630.2135199753928371No Hit
GTACATGGGAAGCAGTGGTATCAAC22240.20984022327603613No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA20320.1917245205471697No Hit
GTATCAACGCAGAGTACATAAGCAG19300.1821005534724594No Hit
GGATACCACGTGTCCCGCCCTACTC18880.17813774350051986No Hit
TATCAACGCAGAGTACATAAGCAGT18280.17247658639774913No Hit
GGTATCAACGCAGAGTACATAAGCA17260.16285261932303885No Hit
GGTATCAACGCAGAGTACATGGGGG16750.1580406357856837No Hit
GTACATGATAAGCAGTGGTATCAAC15510.14634091110662412No Hit
ACCCTGTATCGCGCGCCTTTCCAGA15320.14454821135741336No Hit
AAGCAGTGGTATCAACGCAGAGTAC15170.14313292208172068No Hit
ATCATGTACTCTGCGTTGATACCAC14400.13586777046649823No Hit
GTGGTATCAACGCAGAGTACATAAG13070.12331887222202303No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12770.12048829367063765No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN12240.11548760489652349No Hit
ACATAAGCAGTGGTATCAACGCAGA12070.11388361038407177No Hit
GTACTCTGCGTTGATACCACTGCTT12040.11360055252893322No Hit
GCAGTGGTATCAACGCAGAGTACAT11630.10973209517537322No Hit
CCCATATTCAGACAGGATACCACGT11600.10944903732023467No Hit
ATCATTAACTGAATCCATAGGTTAA11390.1074676323342649No Hit
CCACTGCTTATGTACTCTGCGTTGA10750.1014290647579761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAC250.00603499518.99772610
AAGTCCG607.6306605E-817.4261325
TAGGGAC402.7486496E-416.6340355
TGTCCCT402.7625862E-416.6230110
TGCGGGA350.00216308916.2930222
CGACGGA350.002171315516.2837669
TCTCTGA456.7271653E-414.787211
CGATGCC456.765926E-414.77601059
GGACGCC456.7707844E-414.77461117
GTTGTAA400.00525532614.2590951
TTAGACA602.5536048E-514.2577444
AGCACGA400.00525863114.2577445
GGACTTC602.5696205E-514.2482966
ACGACGG400.00528181214.2482968
CCGGATT400.00529011214.24492419
TAAGCGA1152.5465852E-1114.04113413
GTAAGAC551.9517742E-413.8243823
CTCTCGC551.9651467E-413.8132619
CTTACTA500.001493933513.3059683
TAATTGC500.001500344613.2984096
Produced by FastQC (version 0.11.5)