##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1059854 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.099116482081495 31.0 28.0 33.0 22.0 34.0 2 29.164422646892874 31.0 28.0 33.0 20.0 34.0 3 29.196276090857797 31.0 28.0 34.0 22.0 34.0 4 32.02163882949916 35.0 32.0 37.0 17.0 37.0 5 32.91615920683415 35.0 33.0 37.0 25.0 37.0 6 32.98551215544783 35.0 33.0 37.0 24.0 37.0 7 32.99887248621037 35.0 33.0 37.0 24.0 37.0 8 32.91801040520676 35.0 33.0 37.0 23.0 37.0 9 33.53956299641271 37.0 32.0 39.0 17.0 39.0 10 33.67035931364131 37.0 32.0 39.0 18.0 39.0 11 34.09174188142895 38.0 33.0 39.0 22.0 39.0 12 34.16310171023556 38.0 33.0 39.0 23.0 39.0 13 33.950874365714526 38.0 33.0 39.0 21.0 39.0 14 34.40727873839227 38.0 32.0 41.0 17.0 41.0 15 34.72507628409196 38.0 33.0 41.0 19.0 41.0 16 34.65663761235038 38.0 33.0 41.0 19.0 41.0 17 34.434096583114275 38.0 32.0 40.0 18.0 41.0 18 34.32547596178342 38.0 32.0 40.0 18.0 41.0 19 34.716162792233646 39.0 33.0 40.0 20.0 41.0 20 34.72852487229373 39.0 33.0 41.0 20.0 41.0 21 34.76538938382079 39.0 33.0 41.0 21.0 41.0 22 34.69422580846041 39.0 33.0 41.0 21.0 41.0 23 34.41121512963106 38.0 32.0 40.0 19.0 41.0 24 34.747535037844834 39.0 33.0 41.0 22.0 41.0 25 33.44004362865074 38.0 30.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2529221870016407 1101 2 0.28216487966619397 1101 3 0.27379592575751843 1101 4 0.398011220634654 1101 5 0.17617258394510316 1101 6 0.058655901588331005 1101 7 0.033174220373432206 1101 8 0.1998348434972641 1101 9 0.340979103980672 1101 10 0.66814021171637 1101 11 0.3365200179843484 1101 12 0.3148791156245778 1101 13 0.33811803374585025 1101 14 1.1010788433864178 1101 15 0.5010844344158301 1101 16 0.2128048721840159 1101 17 0.3273484135348852 1101 18 0.618940458093995 1101 19 0.5915325935228566 1101 20 0.39830559319022285 1101 21 0.38101875884621705 1101 22 0.24882641971177577 1101 23 0.3644434970004511 1101 24 0.20121785671926062 1101 25 0.15866665950017023 1102 1 -0.26821556209585395 1102 2 0.05735737868026547 1102 3 -0.039363547200832016 1102 4 -0.07767428120846986 1102 5 -0.21398960605155537 1102 6 -0.1328999664910242 1102 7 -0.015482778625475646 1102 8 -0.08620130641355672 1102 9 0.07403495144693295 1102 10 0.4433358221399004 1102 11 0.05261401677556421 1102 12 0.0393746978120717 1102 13 -0.06911762674025823 1102 14 0.0665169181706986 1102 15 0.019653326077332167 1102 16 -0.04416099020923525 1102 17 -0.03380027216686443 1102 18 0.2142802667889825 1102 19 0.21472924004573457 1102 20 0.09170847171068175 1102 21 0.054572011785914754 1102 22 0.042353287899246084 1102 23 7.317877554413599E-4 1102 24 -0.11516736999747934 1102 25 0.26465137781260495 1103 1 -0.4750906692684467 1103 2 -0.0884435119094178 1103 3 -0.44204191148910255 1103 4 -0.2153420814798821 1103 5 -0.3826790705106049 1103 6 -0.4008182538400433 1103 7 -0.37201000647692695 1103 8 -0.3965746821426208 1103 9 0.2870094884031431 1103 10 0.2702942775820958 1103 11 -0.11213615179092074 1103 12 -0.210665455538809 1103 13 -0.23377895359318757 1103 14 -0.08433941507961151 1103 15 -0.03072186528151377 1103 16 -0.06087758669482213 1103 17 0.18943257304138683 1103 18 0.1765733606454063 1103 19 -0.1464146532697228 1103 20 -0.039570872241760924 1103 21 -0.22975250798388913 1103 22 -0.28595175962912833 1103 23 -2.3059598942548476 1103 24 -1.0495059725367213 1103 25 -0.42270221763870097 1104 1 -0.1832480257643141 1104 2 0.11195211370874603 1104 3 -0.05223661491832843 1104 4 -0.4924768895030205 1104 5 -0.1348411832639087 1104 6 -0.08840292194108201 1104 7 -0.09448535409465819 1104 8 0.11785737165746468 1104 9 -2.3598969911382284 1104 10 -0.554012479147211 1104 11 -0.47511953145244945 1104 12 -0.319038281121351 1104 13 -0.06488572094751532 1104 14 0.08668585214736879 1104 15 -0.14034234906351628 1104 16 0.10454454677726233 1104 17 0.3548828315574113 1104 18 0.5632458398211568 1104 19 0.07776538451158643 1104 20 0.025339385430520167 1104 21 -0.006340440152527549 1104 22 -0.07970924987520789 1104 23 0.05217816533586728 1104 24 0.20372098563039742 1104 25 -0.045964128985431785 1105 1 -0.034207044735072856 1105 2 0.049932625062226066 1105 3 -0.0042332609721995595 1105 4 0.32759993066011717 1105 5 -0.030614532955944185 1105 6 -0.06419162333506989 1105 7 -0.11810863319352904 1105 8 -0.06735258906930852 1105 9 -2.0607977476257098 1105 10 -0.4998214336901441 1105 11 -0.14153213464486214 1105 12 0.03501728094206413 1105 13 0.17206918574003538 1105 14 -0.028391800336954987 1105 15 0.21340032188600588 1105 16 0.13931014046887924 1105 17 -1.3562215661811408 1105 18 -0.31201310454896714 1105 19 -0.153999845424849 1105 20 -0.10914984679942563 1105 21 0.07685545742880606 1105 22 -0.03512997282322772 1105 23 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0.6792454941255883 1108 21 0.9380492024433735 1108 22 1.0375190469584012 1108 23 0.9812050903153704 1108 24 0.7303172751013491 1108 25 0.8641051901068408 1109 1 0.41261383054157363 1109 2 0.49408376377857977 1109 3 0.4446551519641879 1109 4 0.6011731440534156 1109 5 0.721157438201331 1109 6 0.4589667974545364 1109 7 0.5802247001398015 1109 8 0.37651547689770837 1109 9 0.7984669057618845 1109 10 0.6520030112302493 1109 11 0.4892892403819076 1109 12 0.45572764004822375 1109 13 0.4961282276476524 1109 14 1.0217909654230226 1109 15 0.5090382172240524 1109 16 0.7379076274396823 1109 17 1.1230687886414472 1109 18 0.7983092342816178 1109 19 0.6525008328074691 1109 20 0.7292159702920316 1109 21 0.5627785673024377 1109 22 0.5850931299111224 1109 23 0.8596244296880471 1109 24 0.8045851092632006 1109 25 0.6370980320491952 1110 1 0.5767117474145742 1110 2 0.5778776299434476 1110 3 0.6342728601943577 1110 4 0.75627796066561 1110 5 0.8059710792168175 1110 6 0.5517851263311044 1110 7 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2213 18 -0.1913042922884287 2213 19 -0.2924508580137868 2213 20 -0.4295521456499998 2213 21 -0.17620693994393832 2213 22 -0.04094551743187935 2213 23 0.25566307703104485 2213 24 -0.1986757603020166 2213 25 -0.38123530128414274 2214 1 0.3873814982541077 2214 2 0.40586433578232217 2214 3 0.4199987552574953 2214 4 0.9381580536604694 2214 5 0.5369042907652855 2214 6 0.5780520947407837 2214 7 0.3812809490094011 2214 8 0.29834614051326724 2214 9 0.4794036267665476 2214 10 0.5077291036913465 2214 11 0.3441081792575602 2214 12 0.30579220082264413 2214 13 -0.06405892782507294 2214 14 0.34635661531647344 2214 15 0.2029761099163352 2214 16 0.2900392339099156 2214 17 0.31858712280047286 2214 18 0.5099249836505635 2214 19 0.42551049726444035 2214 20 0.4421018604993989 2214 21 0.48796198752938835 2214 22 0.598516109143155 2214 23 0.6178852992532669 2214 24 0.7073768712769279 2214 25 0.6870889888225662 2215 1 0.464920791815036 2215 2 0.41252044707627533 2215 3 0.5777366440050535 2215 4 0.9486476035449805 2215 5 0.6416041001162753 2215 6 0.48351894760897096 2215 7 0.4593503831999115 2215 8 0.4206067099696966 2215 9 0.8761480651821714 2215 10 0.5816632630715475 2215 11 0.47021582626928904 2215 12 0.4091417526640484 2215 13 0.21495930391279927 2215 14 0.47467288511891326 2215 15 0.5085630449741103 2215 16 0.3606303644318416 2215 17 0.5740215090025842 2215 18 0.8184042687316051 2215 19 0.3428405809661754 2215 20 -0.4228581565789611 2215 21 0.22996792344404327 2215 22 -0.47819506205846807 2215 23 0.5266284686521772 2215 24 0.59233516865973 2215 25 0.7961144254918153 2216 1 0.027932964776603342 2216 2 -0.5124077461279661 2216 3 -0.11323882119225814 2216 4 0.2908361313916892 2216 5 0.2890408743140256 2216 6 0.177516485661684 2216 7 0.18984726668728058 2216 8 0.06696166024227068 2216 9 0.3301409138114906 2216 10 0.09663620119069805 2216 11 -0.004924367950991382 2216 12 -0.29087062220324356 2216 13 -0.405969853228477 2216 14 -0.24326025151916042 2216 15 -0.02458531665858743 2216 16 -0.020679026789821364 2216 17 -0.1373855134089581 2216 18 0.4029441516262864 2216 19 0.12262330434881363 2216 20 -1.0769995036158306 2216 21 -1.2257222359312223 2216 22 -1.3586186733052728 2216 23 -1.744450815168662 2216 24 -1.3578950690028506 2216 25 -1.3884482532110383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2229.0 3 266.0 4 225.0 5 187.0 6 216.0 7 240.0 8 146.0 9 339.0 10 782.0 11 1653.0 12 3286.0 13 5673.0 14 8511.0 15 11097.0 16 13504.0 17 14741.0 18 14567.0 19 13860.0 20 13132.0 21 12588.0 22 11930.0 23 11523.0 24 11488.0 25 11831.0 26 12148.0 27 13199.0 28 14741.0 29 17024.0 30 20608.0 31 25361.0 32 32681.0 33 43222.0 34 57580.0 35 79628.0 36 118812.0 37 183533.0 38 277303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.634325650310316 22.400932092559135 12.13024349311703 20.834498764013517 2 15.115979771674287 25.73462056748327 36.608386579050965 22.541013081791476 3 18.50122329023413 33.21232985661372 27.375947765885726 20.91049908726642 4 12.7567830696249 23.803557341076484 31.72172391161671 31.7179356776819 5 11.197057104782868 44.33806167843149 30.626587020081814 13.83829419670383 6 26.201717124190928 40.91740895824202 18.288063724260414 14.592810193306644 7 22.563094185432245 40.23893331111696 18.83907456181043 18.358897941640365 8 23.30133768148466 38.94748603563553 19.613457679855994 18.13771860302382 9 25.810901392875806 23.213744707602118 18.05540828151391 32.919945618008164 10 15.982221666609842 33.83063798191482 26.753683141836476 23.433457209638863 11 28.185855404790317 31.97016911172578 19.268384311625095 20.57559117185881 12 22.321724427175937 33.93157732296466 23.909215362155837 19.837482887703572 13 26.59592893669096 28.413871560952845 24.807420931137187 20.182778571219 14 23.2830215110963 30.326234905033516 23.500536688885145 22.890206894985035 15 26.094338264815274 31.96884303342954 22.150478376474332 19.78634032528085 16 20.688418211176504 32.94836532145555 24.627604496951363 21.73561197041659 17 19.29746979281088 34.57851975303966 24.09226923222605 22.03174122192341 18 19.218757839353483 30.58012872395915 29.77718959946761 20.42392383721976 19 21.209504793769 33.480549898841865 25.778898706482355 19.53104660090678 20 22.5292208099488 30.776161497619746 27.216664616083513 19.477953076347944 21 23.4716648157458 31.857898508943595 24.473055609994404 20.197381065316204 22 22.052435633669795 30.6706060267667 24.61185252761937 22.665105811944134 23 23.271844053902154 29.284553184000423 25.226374283502924 22.217228478594503 24 21.76210628141869 30.620626466520967 24.52387465369045 23.093392598369896 25 22.115016028871846 31.762910884772904 25.047537649155586 21.074535437199664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33196.0 1 33196.0 2 21829.0 3 10462.0 4 10462.0 5 10462.0 6 10860.5 7 11259.0 8 11259.0 9 11259.0 10 11306.0 11 11353.0 12 11353.0 13 11353.0 14 12364.5 15 13376.0 16 13376.0 17 13376.0 18 17028.0 19 20680.0 20 20680.0 21 20680.0 22 26596.5 23 32513.0 24 32513.0 25 32513.0 26 40744.0 27 48975.0 28 48975.0 29 48975.0 30 55751.5 31 62528.0 32 62528.0 33 62528.0 34 65413.5 35 68299.0 36 68299.0 37 68299.0 38 73013.5 39 77728.0 40 77728.0 41 77728.0 42 115228.0 43 152728.0 44 152728.0 45 152728.0 46 143495.5 47 134263.0 48 134263.0 49 134263.0 50 128402.5 51 122542.0 52 122542.0 53 122542.0 54 108907.0 55 95272.0 56 95272.0 57 95272.0 58 81392.0 59 67512.0 60 67512.0 61 67512.0 62 58007.5 63 48503.0 64 48503.0 65 48503.0 66 36396.0 67 24289.0 68 24289.0 69 24289.0 70 19187.0 71 14085.0 72 14085.0 73 14085.0 74 10441.0 75 6797.0 76 6797.0 77 6797.0 78 4580.5 79 2364.0 80 2364.0 81 2364.0 82 1550.0 83 736.0 84 736.0 85 736.0 86 539.5 87 343.0 88 343.0 89 343.0 90 188.0 91 33.0 92 33.0 93 33.0 94 24.5 95 16.0 96 16.0 97 16.0 98 9.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1500206632234251 2 0.29419146410732044 3 0.3477837513468836 4 0.37297590045421347 5 0.3795805837407794 6 0.3352348531024084 7 0.33740496332513725 8 0.35599242914590123 9 0.3417451837705948 10 0.3738250740196291 11 0.2922100591213507 12 0.3392920156927275 13 0.30900482519290395 14 0.31853443964923467 15 0.3568416027113169 16 0.3128732825464639 17 0.35986088649946124 18 0.3288188750526016 19 0.3384428421273119 20 0.30607989402313907 21 0.34183953638897435 22 0.29890909502629603 23 0.2807933922974296 24 0.31674173990002397 25 0.31353375087512053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1059854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.21788513812223 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42292006554314 19.77660190409008 2 7.231723225991674 3.647375313274976 3 3.036632272443826 2.297323315596105 4 1.864296407889319 1.8805445071026685 5 1.2440857126351779 1.5686605301606427 6 0.9733133740791907 1.4726942922556334 7 0.7522484398547804 1.327908032611265 8 0.6066384378307285 1.223851075646817 9 0.5016139331070342 1.1384678293897423 >10 4.468978524847529 23.47090707429483 >50 0.5683223778707326 9.785151505781956 >100 0.28774384294723787 13.285289600003262 >500 0.024890030975776797 4.369181250605604 >1k 0.013953502213693054 7.185535208975599 >5k 0.001885608407255818 3.3117408621705398 >10k+ 7.542433629023273E-4 4.258767698040345 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 29248 2.759625382363986 No Hit GTACATAAGCAGTGGTATCAACGCA 15533 1.46557922128897 No Hit GCTTATGTACTCTGCGTTGATACCA 9244 0.872195604300215 No Hit GTATCAACGCAGAGTACTTTTTTTT 8061 0.7605764567572515 No Hit CTTATGTACTCTGCGTTGATACCAC 6443 0.6079139202192 No Hit GGTATCAACGCAGAGTACTTTTTTT 5738 0.5413953242616436 No Hit TATCAACGCAGAGTACTTTTTTTTT 5337 0.503559924291459 No Hit GTGGTATCAACGCAGAGTACATGGG 4580 0.4321349921781679 No Hit CCCATGTACTCTGCGTTGATACCAC 4340 0.40949036376708486 No Hit GAGTACATAAGCAGTGGTATCAACG 4337 0.4092073059119464 No Hit TATGTACTCTGCGTTGATACCACTG 3481 0.3284414645790835 No Hit GCGTTGATACCACTGCTTATGTACT 3272 0.30872176733776535 No Hit CATAAGCAGTGGTATCAACGCAGAG 2881 0.27182989355137593 No Hit CTGCTTATGTACTCTGCGTTGATAC 2615 0.24673209706242558 No Hit CATGTACTCTGCGTTGATACCACTG 2610 0.24626033397052802 No Hit ACGCAGAGTACATAAGCAGTGGTAT 2530 0.238712124500167 No Hit ACTCTGCGTTGATACCACTGCTTAT 2425 0.22880509957031817 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2413 0.22767286814976403 No Hit GTACTGGTTCACTATCGGTCAGTCA 2346 0.22135124271833667 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2279 0.21502961728690934 No Hit ATACAGGGTGACAGCCCCGTACACA 2263 0.2135199753928371 No Hit GTACATGGGAAGCAGTGGTATCAAC 2224 0.20984022327603613 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 2032 0.1917245205471697 No Hit GTATCAACGCAGAGTACATAAGCAG 1930 0.1821005534724594 No Hit GGATACCACGTGTCCCGCCCTACTC 1888 0.17813774350051986 No Hit TATCAACGCAGAGTACATAAGCAGT 1828 0.17247658639774913 No Hit GGTATCAACGCAGAGTACATAAGCA 1726 0.16285261932303885 No Hit GGTATCAACGCAGAGTACATGGGGG 1675 0.1580406357856837 No Hit GTACATGATAAGCAGTGGTATCAAC 1551 0.14634091110662412 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1532 0.14454821135741336 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1517 0.14313292208172068 No Hit ATCATGTACTCTGCGTTGATACCAC 1440 0.13586777046649823 No Hit GTGGTATCAACGCAGAGTACATAAG 1307 0.12331887222202303 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1277 0.12048829367063765 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 1224 0.11548760489652349 No Hit ACATAAGCAGTGGTATCAACGCAGA 1207 0.11388361038407177 No Hit GTACTCTGCGTTGATACCACTGCTT 1204 0.11360055252893322 No Hit GCAGTGGTATCAACGCAGAGTACAT 1163 0.10973209517537322 No Hit CCCATATTCAGACAGGATACCACGT 1160 0.10944903732023467 No Hit ATCATTAACTGAATCCATAGGTTAA 1139 0.1074676323342649 No Hit CCACTGCTTATGTACTCTGCGTTGA 1075 0.1014290647579761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.435261837951266E-5 0.0 0.0 0.0 0.0 2 9.435261837951266E-5 0.0 0.0 0.0 0.0 3 9.435261837951266E-5 0.0 0.0 0.0 0.0 4 9.435261837951266E-5 0.0 0.0 0.0 0.0 5 9.435261837951266E-5 0.0 0.0 0.0 0.0 6 1.887052367590253E-4 0.0 0.0 0.0 0.0 7 1.887052367590253E-4 0.0 0.0 0.0 0.0 8 3.774104735180506E-4 0.0 0.0 0.0 0.0 9 3.774104735180506E-4 0.0 0.0 0.0 0.0 10 3.774104735180506E-4 0.0 0.0 9.435261837951266E-5 0.0 11 3.774104735180506E-4 0.0 0.0 1.887052367590253E-4 0.0 12 3.774104735180506E-4 0.0 0.0 1.887052367590253E-4 0.0 13 3.774104735180506E-4 0.0 0.0 3.774104735180506E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACTAC 25 0.006034995 18.997726 10 AAGTCCG 60 7.6306605E-8 17.426132 5 TAGGGAC 40 2.7486496E-4 16.634035 5 TGTCCCT 40 2.7625862E-4 16.62301 10 TGCGGGA 35 0.002163089 16.293022 2 CGACGGA 35 0.0021713155 16.283766 9 TCTCTGA 45 6.7271653E-4 14.78721 1 CGATGCC 45 6.765926E-4 14.7760105 9 GGACGCC 45 6.7707844E-4 14.774611 17 GTTGTAA 40 0.005255326 14.259095 1 TTAGACA 60 2.5536048E-5 14.257744 4 AGCACGA 40 0.005258631 14.257744 5 GGACTTC 60 2.5696205E-5 14.248296 6 ACGACGG 40 0.005281812 14.248296 8 CCGGATT 40 0.005290112 14.244924 19 TAAGCGA 115 2.5465852E-11 14.041134 13 GTAAGAC 55 1.9517742E-4 13.824382 3 CTCTCGC 55 1.9651467E-4 13.81326 19 CTTACTA 50 0.0014939335 13.305968 3 TAATTGC 50 0.0015003446 13.298409 6 >>END_MODULE