FastQCFastQC Report
Wed 25 May 2016
SRR1295201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059854
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT265462.5046846075025426No Hit
GGTATCAACGCAGAGTACTTTTTTT191751.809211457427155No Hit
GTACATAAGCAGTGGTATCAACGCA172731.629752777269322No Hit
TATCAACGCAGAGTACTTTTTTTTT168851.593143961338071No Hit
GCTTATGTACTCTGCGTTGATACCA100590.9490929882795177No Hit
ACGCAGAGTACTTTTTTTTTTTTTT85610.8077527659470078No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78820.7436873380673187No Hit
CTTATGTACTCTGCGTTGATACCAC75210.7096260428323146No Hit
CCCATGTACTCTGCGTTGATACCAC50240.47402755473867153No Hit
GTGGTATCAACGCAGAGTACATGGG48240.455157031062769No Hit
GAGTACTTTTTTTTTTTTTTTTTTT48090.4537417417870763No Hit
GAGTACATAAGCAGTGGTATCAACG47520.4483636425394441No Hit
CATAAGCAGTGGTATCAACGCAGAG34870.3290075802893606No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34770.3280640541055655No Hit
TATGTACTCTGCGTTGATACCACTG34660.32702617530339084No Hit
GCGTTGATACCACTGCTTATGTACT34610.3265544122114933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33730.3182513817940962No Hit
CATGTACTCTGCGTTGATACCACTG29580.2790950451665984No Hit
CTGCTTATGTACTCTGCGTTGATAC28180.2658856785934667No Hit
ACGCAGAGTACATAAGCAGTGGTAT27510.2595640531620393No Hit
ACTCTGCGTTGATACCACTGCTTAT27470.25918664268852126No Hit
ATACAGGGTGACAGCCCCGTACACA27010.25484642224306364No Hit
ATCAACGCAGAGTACTTTTTTTTTT26840.25324242773061195No Hit
GTGGTATCAACGCAGAGTACTTTTT26500.2500344387057085No Hit
GTACATGGGAAGCAGTGGTATCAAC25060.2364476616590587No Hit
GTACTGGTTCACTATCGGTCAGTCA24720.2332396726341553No Hit
GTATCAACGCAGAGTACATAAGCAG22080.20833058138196395No Hit
TATCAACGCAGAGTACATAAGCAGT20490.19332851505962143No Hit
GGTATCAACGCAGAGTACATGGGGG19990.18861088414064578No Hit
GGATACCACGTGTCCCGCCCTACTC19790.18672383177305554No Hit
GGTATCAACGCAGAGTACATAAGCA18970.1789869170659355No Hit
AAGCAGTGGTATCAACGCAGAGTAC17410.16426790859873153No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17190.16219215099438225No Hit
ATCATGTACTCTGCGTTGATACCAC16940.15983333553489443No Hit
ACATAAGCAGTGGTATCAACGCAGA15150.14294421684496167No Hit
GTACATGATAAGCAGTGGTATCAAC15070.14218939589792556No Hit
GTGGTATCAACGCAGAGTACATAAG14680.13850964378112457No Hit
GCAGTGGTATCAACGCAGAGTACAT13400.12643250862854696No Hit
GTACTCTGCGTTGATACCACTGCTT12460.11756336250087276No Hit
ATCATTAACTGAATCCATAGGTTAA12340.1164311310803186No Hit
CCACTGCTTATGTACTCTGCGTTGA11960.11284573158189713No Hit
CAACGCAGAGTACTTTTTTTTTTTT11820.11152479492458395No Hit
CCCATATTCAGACAGGATACCACGT11740.11076997397754784No Hit
GGTATCAACGCAGAGTACATGGGAA11540.10888292160995759No Hit
GAGTACATGGGAAGCAGTGGTATCA11280.10642975353209028No Hit
GGTATCAACGCAGAGTACATGGGCA10940.10322176450718683No Hit
CACACACACTGATTCAGGCTCTGGG10910.1029387066520483No Hit
GATACCACTGCTTATGTACTCTGCG10880.10265564879690978No Hit
AACGCAGAGTACTTTTTTTTTTTTT10860.10246694356015074No Hit
GCCCAGAGCCTGAATCAGTGTGTGT10770.10161776999473512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGCG250.006027724619.00175
TCGACTT401.2841119E-519.00080113
CTTTCGT250.00603101118.99990712
GTCGACT453.5373247E-516.88880512
ATGGCTA402.7581956E-416.6264865
ATCGATC402.7591886E-416.62570213
ATCATTC551.1276856E-515.5453788
TATGGCT400.005276159414.2506014
AAGCGCT400.005277811514.2499297
TCGGACA400.005277811514.24992914
TAACTGC400.005277811514.2499296
AAGTCAC400.005277811514.2499297
GTCTAAC759.634077E-713.937211
AGGACCG551.9577403E-413.8194185
GCACTGA551.9593083E-413.8181136
CGATCAA551.9608773E-413.8168115
TACACTG707.250632E-613.5726425
CTATGAC1205.0931703E-1113.4589013
GTGCTAG500.001495858913.3037011
ATGGACC500.001498521213.3005614
Produced by FastQC (version 0.11.5)