FastQCFastQC Report
Wed 25 May 2016
SRR1295200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4548108
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG56560.12435940395434761No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51070.11228845049413953No Hit
GTATCAACGCAGAGTACTTTTTTTT48740.10716544110210223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.011.4949561
ACCGTCC1053.473584E-610.856318
TGGTATC12550.010.677912
GTGGTAT13900.010.5989141
CCGTCCA1606.193659E-910.0928649
CATACCG951.638074E-410.0044245
CTAGGAC4100.09.2724953
GTCCTAA3700.09.2479451
GTAGACG950.00181698459.004284
GTCTTAG4550.08.7736911
AACAGCG2002.5460395E-88.5492517
ATACCGT1701.616052E-68.3814336
CCAGTAC4450.08.32961753
ACACCGT1606.465876E-68.3115876
GTACTAA3900.08.042551
TATTCCG2252.1698725E-88.0257715
GTATCAA52800.08.0107011
GTGTAGG4700.07.8869881
CTATACT6400.07.87092834
TGTACCG1451.5553976E-47.86554675
Produced by FastQC (version 0.11.5)