##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295200_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4548108
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.65078709652453	31.0	30.0	31.0	25.0	34.0
2	29.923396058316996	31.0	30.0	33.0	25.0	34.0
3	29.910882283358266	31.0	30.0	34.0	25.0	34.0
4	33.34968518777478	35.0	33.0	37.0	27.0	37.0
5	33.396587987796245	35.0	33.0	37.0	27.0	37.0
6	33.70655204317927	35.0	35.0	37.0	28.0	37.0
7	33.63514081020064	35.0	35.0	37.0	28.0	37.0
8	33.80961204087502	36.0	35.0	37.0	28.0	37.0
9	34.72281638870493	38.0	34.0	39.0	26.0	39.0
10	34.777976028713475	38.0	34.0	39.0	27.0	39.0
11	35.170972852887395	38.0	35.0	39.0	27.0	39.0
12	35.16292950827025	39.0	35.0	39.0	27.0	39.0
13	35.08296328935021	38.0	35.0	39.0	27.0	39.0
14	35.642992206869316	39.0	34.0	41.0	25.0	41.0
15	36.01649894857378	39.0	35.0	41.0	27.0	41.0
16	35.987928606796494	39.0	35.0	41.0	27.0	41.0
17	35.63717528255705	39.0	34.0	41.0	26.0	41.0
18	35.534593725566765	39.0	34.0	41.0	25.0	41.0
19	35.98605661958775	39.0	35.0	41.0	27.0	41.0
20	35.998104706396596	39.0	35.0	41.0	27.0	41.0
21	36.05178944739219	39.0	35.0	41.0	27.0	41.0
22	35.958386652207906	39.0	35.0	41.0	27.0	41.0
23	35.655490810684356	39.0	34.0	41.0	25.0	41.0
24	35.94314734830395	39.0	35.0	41.0	27.0	41.0
25	34.503581269398175	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.25423341716912873
1101	2	0.07263111638018316
1101	3	-0.2669966227932292
1101	4	-0.10724697045488796
1101	5	-0.33608112492093767
1101	6	-0.5148002901590516
1101	7	-0.40068894368315
1101	8	-0.23066915110801745
1101	9	-0.10330593889609929
1101	10	-0.02182132737771525
1101	11	-0.10329122584233374
1101	12	-0.1397819929774471
1101	13	-0.11042684942739811
1101	14	0.3818547404412911
1101	15	0.15533657629320885
1101	16	-0.061394397130229095
1101	17	0.035843175456825804
1101	18	0.24459019642047508
1101	19	0.014966940006985396
1101	20	-0.027824510238438904
1101	21	-0.035326776293359785
1101	22	-0.09957143549519287
1101	23	0.0912762282686117
1101	24	-0.13335962932445256
1101	25	-0.199856784350537
1102	1	-0.3243013516875699
1102	2	-0.04014902977462498
1102	3	-0.2135986228668294
1102	4	-0.1548857359897724
1102	5	-0.6932312294617802
1102	6	-0.5532804441008778
1102	7	-0.5758861268843987
1102	8	-0.456797611616075
1102	9	-0.47082751112588284
1102	10	0.08374868907034028
1102	11	-0.127408534969625
1102	12	-0.20339796442004854
1102	13	-0.3086022647106077
1102	14	-0.15677377592237463
1102	15	-0.14778754249149983
1102	16	-0.2664090013109828
1102	17	-0.3309877533646244
1102	18	-0.01703115094306895
1102	19	-0.22058637218879795
1102	20	-0.09355866531350188
1102	21	-0.1898255695838671
1102	22	-0.3222188752817061
1102	23	-0.21746612053105707
1102	24	-0.2209310527578765
1102	25	-0.3544975948659328
1103	1	-0.5465955972778112
1103	2	-0.24552491303608903
1103	3	-0.47682299932462513
1103	4	-0.3717741809195232
1103	5	-0.745121223466434
1103	6	-0.7127869379526004
1103	7	-0.788358897423052
1103	8	-0.34037468426326
1103	9	-0.007625911923618389
1103	10	0.051188645803840416
1103	11	-0.18268060331373448
1103	12	-0.29083293768940877
1103	13	-0.22286975086268512
1103	14	-0.3074201230724327
1103	15	-0.2880493124147918
1103	16	-0.29074682321673606
1103	17	-0.17699985861753476
1103	18	0.1916995317052823
1103	19	-0.14975415108280288
1103	20	-0.08619183709894429
1103	21	-0.013709064752539746
1103	22	-0.17287235070798346
1103	23	-2.3828554201419223
1103	24	-1.0915873929442128
1103	25	-0.40488893010995497
1104	1	-0.3395480245476179
1104	2	-0.024135682172857997
1104	3	-0.2842740641122816
1104	4	-0.5484650873941135
1104	5	-0.5452420569121017
1104	6	-0.552013924559418
1104	7	-0.4815653233996926
1104	8	-0.36300438329516993
1104	9	-2.6654479840865903
1104	10	-0.9038233996518983
1104	11	-0.6601222349804345
1104	12	-0.42012482426414266
1104	13	-0.29216577968308144
1104	14	-0.05593627703283488
1104	15	-0.27001989505239266
1104	16	-0.17714250554980993
1104	17	0.11904814548690013
1104	18	0.18806639220851906
1104	19	-0.11119327793921485
1104	20	-0.1693770915977808
1104	21	-0.24193892357160252
1104	22	-0.14625537131360034
1104	23	-0.10597250107835521
1104	24	-0.22210073547402942
1104	25	-0.15999921530671912
1105	1	-0.3998652428736449
1105	2	-0.302208308872256
1105	3	-0.3694113831872059
1105	4	-0.21123675235703843
1105	5	-0.4378031380141678
1105	6	-0.405511090619207
1105	7	-0.5165016689718342
1105	8	-0.5434430241023804
1105	9	-2.3915560403338816
1105	10	-0.8521373226068363
1105	11	-0.6600523096187558
1105	12	-0.42550474830422047
1105	13	-0.3977290156862665
1105	14	-0.38544151392498094
1105	15	-0.4466134463273548
1105	16	-0.42536753289378737
1105	17	-1.8550992422763528
1105	18	-0.48797663526448076
1105	19	-0.4536130224291668
1105	20	-0.5185320396758399
1105	21	-0.34679783219153393
1105	22	-0.32959685086096613
1105	23	-0.30377288637857447
1105	24	-0.3302906523536535
1105	25	-1.0722286919412554
1106	1	-0.43092240948271154
1106	2	-0.5700965085145526
1106	3	-0.5144781670368133
1106	4	-0.4397364928405665
1106	5	-0.39333217397850007
1106	6	-0.44713646479466007
1106	7	-0.48698723306604563
1106	8	-0.47057789357996427
1106	9	-3.2619807750892313
1106	10	-0.9014987925610356
1106	11	-0.7757177589357411
1106	12	-0.4646614736208434
1106	13	-0.529488555173586
1106	14	-0.738517489820083
1106	15	-0.6145188877304193
1106	16	-0.6683027341353949
1106	17	-0.7070827949589571
1106	18	-0.16615650039729246
1106	19	-0.41445063886509104
1106	20	-0.3926621000245447
1106	21	-0.3574445558882502
1106	22	-0.33393793459463694
1106	23	-0.1624346968702639
1106	24	-0.5057379654235987
1106	25	-0.7037486141854927
1107	1	-0.3740471967582053
1107	2	-0.1501462751639977
1107	3	-0.20647811189919807
1107	4	-1.4224107356465652
1107	5	-0.4773158608384449
1107	6	-0.30626510146802843
1107	7	-0.19496699541549845
1107	8	-0.12761217171860295
1107	9	0.10152626690445743
1107	10	0.39354140569005835
1107	11	0.007515953192182678
1107	12	-0.6330676370981791
1107	13	-0.2876730963941725
1107	14	0.05428499209892834
1107	15	-0.6914569490811644
1107	16	-1.1066229757933286
1107	17	-0.21002017294996023
1107	18	-0.2710825344844707
1107	19	-0.07977701990652974
1107	20	-0.45679199483426913
1107	21	-0.17675890314535536
1107	22	-0.20969190329569898
1107	23	0.08651105419669847
1107	24	-0.2908548581978181
1107	25	-0.47088765255330856
1108	1	0.2609889943863557
1108	2	0.32122964611539473
1108	3	0.369227456305083
1108	4	0.7337787790408683
1108	5	0.5842687271807208
1108	6	0.43653091528164367
1108	7	0.40788360560755166
1108	8	0.3978043017374304
1108	9	0.7801823949247506
1108	10	0.9786479646070205
1108	11	0.5624193164435596
1108	12	0.49712495840793025
1108	13	0.4858353577100729
1108	14	0.5516617426078767
1108	15	0.623057490465051
1108	16	0.6414105037982623
1108	17	0.7470712570362465
1108	18	0.822096029037148
1108	19	0.6068351021484872
1108	20	0.5955290341197994
1108	21	0.538751281632301
1108	22	0.5785207455569363
1108	23	0.8070912993683237
1108	24	0.656354570309162
1108	25	0.8289151650237088
1109	1	0.3747743858387409
1109	2	0.21872218982504776
1109	3	0.4248180163850961
1109	4	0.7269321553660717
1109	5	0.6215300363368712
1109	6	0.48765303621394196
1109	7	0.5089393812615413
1109	8	0.46270497653537745
1109	9	0.8389575501396038
1109	10	0.6798024715293707
1109	11	0.4389569976981278
1109	12	0.4279912403623527
1109	13	0.3392766944277952
1109	14	0.837930322361629
1109	15	0.530318474073681
1109	16	0.5589858454571157
1109	17	0.8392323993574564
1109	18	0.6062781649272111
1109	19	0.37089508041410824
1109	20	0.4883272696740093
1109	21	0.4852733507600817
1109	22	0.5136717337352081
1109	23	0.7658718658580312
1109	24	0.5654435740216002
1109	25	0.6460955707212506
1110	1	0.39783481290396594
1110	2	0.26826202885041184
1110	3	0.42343734948770617
1110	4	0.6578238142463348
1110	5	0.6094083480489942
1110	6	0.5885763450268584
1110	7	0.5638070924214418
1110	8	0.4998409985221599
1110	9	0.9438965672580153
1110	10	1.0751268611540397
1110	11	0.5854935020417429
1110	12	0.5848184639362373
1110	13	0.5216431172321947
1110	14	0.7862821471946333
1110	15	0.5618772857434848
1110	16	0.5756033046274496
1110	17	0.9766806135268382
1110	18	1.0391566292291046
1110	19	0.6209792212287084
1110	20	0.6330437351494425
1110	21	0.6624159005589974
1110	22	0.629604140578067
1110	23	0.8492172656876065
1110	24	0.6122200230779313
1110	25	0.50795723954257
1111	1	0.2594212158887288
1111	2	0.26236940442047185
1111	3	0.44190800448478385
1111	4	0.6693541857550755
1111	5	0.6358396704913076
1111	6	0.4954174433465468
1111	7	0.39435459389643057
1111	8	0.27578451206028376
1111	9	0.7872156486675763
1111	10	0.7028120511674842
1111	11	0.47564278484868794
1111	12	0.27010046935474463
1111	13	0.2799986177346767
1111	14	0.2757636327573607
1111	15	0.2774945059853948
1111	16	0.16445372414474946
1111	17	0.5578723594580097
1111	18	0.5826336875891869
1111	19	0.411175751726482
1111	20	-0.13759452518227278
1111	21	-0.33443166616852693
1111	22	-0.10695948305089331
1111	23	0.20024377697692586
1111	24	0.19820469851570266
1111	25	0.2695039269186381
1112	1	0.19817176453792484
1112	2	0.08633836062579192
1112	3	0.24505226805906588
1112	4	0.5641946204713406
1112	5	0.570601053333796
1112	6	0.3522057220150856
1112	7	0.3260082097650141
1112	8	0.29531267456002297
1112	9	-1.3729088432537324
1112	10	0.0756766615641169
1112	11	-0.06188611481117334
1112	12	0.08028216859136705
1112	13	0.06589955918622081
1112	14	0.4272674615290768
1112	15	0.16321364948796457
1112	16	0.11677565717213412
1112	17	0.11937061448789876
1112	18	0.17943191193194252
1112	19	0.14145233565115944
1112	20	0.04589803980861973
1112	21	0.01731664559400059
1112	22	0.09765159216236441
1112	23	-0.06832006561560178
1112	24	0.014179086813314257
1112	25	0.08408523037805082
1113	1	0.25983260112961304
1113	2	0.21037681654854623
1113	3	0.31386814644624295
1113	4	0.5024936907689295
1113	5	0.7191018314400495
1113	6	0.45783133791393027
1113	7	0.43477497882695815
1113	8	0.40337481509331496
1113	9	0.2028544293416914
1113	10	0.5323401918130273
1113	11	0.4219957443663489
1113	12	0.1657276906565457
1113	13	0.245615980929756
1113	14	0.007028680220734884
1113	15	0.1476638364023657
1113	16	0.2209001491221798
1113	17	0.664849540112499
1113	18	0.43426593355665233
1113	19	0.3089872391506745
1113	20	0.30903638645396825
1113	21	0.3676971276363119
1113	22	0.39527538082558067
1113	23	-0.01670870928933965
1113	24	0.14381858187373808
1113	25	0.31722209168414395
1114	1	0.4111869577289582
1114	2	0.32104029129327927
1114	3	0.47188757070759024
1114	4	0.60271353798435
1114	5	0.6733700502100817
1114	6	0.5857750168499294
1114	7	0.24963557892371568
1114	8	0.34563786396060436
1114	9	0.038077263807863915
1114	10	0.2787023057793121
1114	11	0.3108048245893471
1114	12	0.2843242352704607
1114	13	0.3858562110307062
1114	14	0.6925134339872869
1114	15	0.36179413234882674
1114	16	0.2863930161209183
1114	17	0.6115875192728453
1114	18	0.5458701595865492
1114	19	0.3606207031118558
1114	20	0.34640289230351584
1114	21	0.2577138492329283
1114	22	0.04828011339098737
1114	23	0.5143052214106163
1114	24	0.44383735372135646
1114	25	0.2658348489595852
1115	1	0.5039341666675199
1115	2	0.3878089601847208
1115	3	0.6022748655918448
1115	4	0.6570284064873633
1115	5	0.8197537600874654
1115	6	0.5645294748466938
1115	7	0.5463019897865777
1115	8	0.4806014755340229
1115	9	0.6867254040636226
1115	10	0.3671474406705997
1115	11	0.3949198798522957
1115	12	0.5440847926707306
1115	13	0.4236238028041015
1115	14	0.4659227818773317
1115	15	0.5029975775032725
1115	16	0.46214072400486117
1115	17	0.7349462724848479
1115	18	0.866203918841137
1115	19	0.5358127415127001
1115	20	0.6347816437306264
1115	21	0.4983527186907608
1115	22	-0.0970589078782993
1115	23	0.4038333496715012
1115	24	0.3887081662791658
1115	25	0.4207386353470568
1116	1	0.64069607286498
1116	2	0.4535105642262707
1116	3	0.6919934665419483
1116	4	0.9382354321760928
1116	5	1.0600605514324997
1116	6	0.8106523547455922
1116	7	0.7843186619376894
1116	8	0.6468907518258433
1116	9	0.9731470295333082
1116	10	0.687611630931876
1116	11	0.5441284023677255
1116	12	0.5339721323883921
1116	13	0.47404366734365055
1116	14	0.9307107435549966
1116	15	0.6184293424324494
1116	16	0.6876310837782569
1116	17	1.064930774447518
1116	18	1.055187750583194
1116	19	0.6763330505482017
1116	20	0.5947526540884454
1116	21	0.7627286621625231
1116	22	0.7191604349206457
1116	23	0.9829847361654629
1116	24	0.6941503307824561
1116	25	0.4546252756796818
1201	1	-0.4199521699322908
1201	2	0.02342447051209362
1201	3	-0.4585132199325379
1201	4	-0.23793978731645637
1201	5	-0.7281699088911182
1201	6	-0.5680229196839051
1201	7	-0.553937450474109
1201	8	-0.49557904667452135
1201	9	-0.05462932203536042
1201	10	0.04431873085120941
1201	11	-0.09277735454658398
1201	12	-0.1110238028983801
1201	13	-0.11037603863791645
1201	14	0.3096457594869193
1201	15	-0.033522947065350195
1201	16	-0.06607850683032268
1201	17	0.015511831369884987
1201	18	0.07379444629702903
1201	19	-0.10488372644560684
1201	20	-0.11758310383719817
1201	21	-0.1782959537556792
1201	22	-0.11391467229339725
1201	23	-0.3385361821592099
1201	24	-0.339017733278034
1201	25	-0.28563579010882023
1202	1	-0.443277727249086
1202	2	-0.38546228001267835
1202	3	-0.6134825215782485
1202	4	-0.3072782930256892
1202	5	-0.8770995104835393
1202	6	-0.7281818704713743
1202	7	-0.7773174673550969
1202	8	-0.5489789083782526
1202	9	0.04557609663346085
1202	10	0.16346088086113042
1202	11	-0.2048665583317586
1202	12	-0.20819785715293904
1202	13	-0.17807146615795943
1202	14	-0.23541624005716955
1202	15	-0.2669024170378478
1202	16	-0.1026754974618811
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2211	9	-1.359036335620651
2211	10	0.17100611861974357
2211	11	0.1537414439303646
2211	12	0.17355873835109037
2211	13	0.2126105640979432
2211	14	0.4329525803396379
2211	15	0.20670989268457163
2211	16	0.3527278674469301
2211	17	0.44044806039259043
2211	18	0.60904051408248
2211	19	0.2087324630904277
2211	20	0.37460208866033895
2211	21	0.30850188387888267
2211	22	0.3684753808255792
2211	23	-1.8710323980196861
2211	24	-0.5375615211781835
2211	25	-0.2347563466828788
2212	1	0.202435876341152
2212	2	-1.3651398591069217
2212	3	-0.2245091230957783
2212	4	0.36747771227877735
2212	5	0.5096569556788708
2212	6	0.47630808328211316
2212	7	0.44602998705250485
2212	8	0.3803393622449107
2212	9	0.846381499331649
2212	10	0.6990625463785562
2212	11	0.497806762911722
2212	12	0.30533999327364114
2212	13	0.27748270901786753
2212	14	0.6801921007737022
2212	15	0.4763601245914586
2212	16	0.4914403904188376
2212	17	0.852367934375259
2212	18	1.0319642560970124
2212	19	0.5935033740906874
2212	20	0.5479035326088777
2212	21	0.5199038616508247
2212	22	0.44340699892306645
2212	23	0.7124547814674926
2212	24	0.49032663844376145
2212	25	0.43691586824667183
2213	1	0.37372735422103887
2213	2	0.1387535539918403
2213	3	0.28965167599468344
2213	4	0.44576267799619274
2213	5	0.5871358901183967
2213	6	0.39131360535566984
2213	7	0.4046679481194744
2213	8	0.29442342531197596
2213	9	0.591879439125762
2213	10	0.5915302879097055
2213	11	0.3515657881066474
2213	12	0.2787492944896002
2213	13	0.07220237941798757
2213	14	-1.38707120420041
2213	15	-0.31847569668234144
2213	16	0.08486532809750713
2213	17	0.4805452272341384
2213	18	0.230900674977903
2213	19	0.11155596781235744
2213	20	0.27310513168237094
2213	21	0.29467075411322696
2213	22	0.3655884273453438
2213	23	0.5398318901929713
2213	24	0.3661813959256932
2213	25	0.04204246409011603
2214	1	0.5818005043377532
2214	2	0.45831102374259913
2214	3	0.4168105458883993
2214	4	0.6962851560814016
2214	5	0.7015726799975326
2214	6	0.5518758669640107
2214	7	0.41162705296735425
2214	8	0.25600849497710243
2214	9	0.4922191935427165
2214	10	0.3819392408155693
2214	11	0.29026844313859357
2214	12	0.11916728941996979
2214	13	0.16039573168833954
2214	14	0.42578498994640057
2214	15	0.15937085863864553
2214	16	0.32453467663140856
2214	17	0.2558396676768311
2214	18	0.4460653755234958
2214	19	0.29123982651085356
2214	20	0.450835712307736
2214	21	0.38994627553894645
2214	22	0.41190245921037416
2214	23	0.42212041274832757
2214	24	0.4552983363039971
2214	25	0.43239825875391347
2215	1	0.3400347833144721
2215	2	0.10203227622967503
2215	3	0.24907229783612905
2215	4	0.5524827612099372
2215	5	0.593866934907588
2215	6	0.42885041878826513
2215	7	0.39209955260033524
2215	8	0.2638369574669497
2215	9	0.5656738598362949
2215	10	0.47503527868313
2215	11	0.25179722839946095
2215	12	0.1908274124134266
2215	13	0.04475319484279083
2215	14	0.19946288144899427
2215	15	0.17071876748646986
2215	16	0.22518534235447873
2215	17	0.39083664993300005
2215	18	0.596064497178098
2215	19	0.18420779325418835
2215	20	-0.7453501564532417
2215	21	-0.19465522124526302
2215	22	-0.9455856862636978
2215	23	-0.005859656339815444
2215	24	0.04289384068129465
2215	25	0.11629785300016238
2216	1	0.2554700158259706
2216	2	-0.45302573036003224
2216	3	0.16581526695120274
2216	4	0.4546400665606214
2216	5	0.526011864577363
2216	6	0.46298231626008146
2216	7	0.4134371373090602
2216	8	0.18744355951538694
2216	9	0.5919377202085769
2216	10	0.2690298577705761
2216	11	0.15494566630896855
2216	12	-0.3379815853646022
2216	13	0.003716827292876701
2216	14	-0.03265520760312057
2216	15	-0.029224707362345725
2216	16	0.07740344802398624
2216	17	0.200485498816235
2216	18	0.48261837005245667
2216	19	0.18870121748873459
2216	20	-0.9808948853016659
2216	21	-1.2334175156050478
2216	22	-1.2020973613747472
2216	23	-1.7616371364667884
2216	24	-1.3709929075258316
2216	25	-1.1530182279871255
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8955.0
3	1381.0
4	1148.0
5	829.0
6	985.0
7	1093.0
8	478.0
9	761.0
10	1867.0
11	4210.0
12	8313.0
13	13513.0
14	19439.0
15	24759.0
16	28864.0
17	31839.0
18	33176.0
19	34214.0
20	35052.0
21	35642.0
22	36651.0
23	38553.0
24	40615.0
25	43671.0
26	48279.0
27	54585.0
28	63456.0
29	75857.0
30	93267.0
31	116687.0
32	150246.0
33	198102.0
34	266038.0
35	367738.0
36	524438.0
37	810161.0
38	1333246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.928924330720555	20.997457682726026	13.50310446312539	23.570513523428026
2	15.913608018200412	21.533412916771216	39.33653960216807	23.216439462860304
3	18.166578820042975	26.216889011655987	30.192491110355586	25.424041057945455
4	13.209380562768441	17.442833554244068	37.55508975243432	31.792696130553168
5	13.617091996408673	37.38809106149838	34.87833365262124	14.11648328947171
6	31.00519089884024	38.14049821068532	17.595732913043125	13.258577977431315
7	25.763681334646186	32.61938963737726	24.110331845818873	17.50659718215768
8	23.52697412233647	37.13111740825295	21.33284288003474	18.009065589375847
9	24.816782112082993	16.844749357135054	21.837838076358345	36.50063045442361
10	15.108522259289895	29.709436130947076	33.90861548267378	21.27342612708925
11	32.12085394279263	25.594379378706115	23.794558882226728	18.49020779627453
12	20.787011267317894	27.40746231743916	31.42249364447024	20.383032770772704
13	26.37468407922507	24.350938438264574	26.72854622517269	22.545831257337664
14	21.01084661762568	23.329834187238212	27.73871415093944	27.920605044196662
15	22.7390964903725	30.85451704060237	24.0852341259561	22.321152343069034
16	22.37409516583664	30.86270781961985	25.381320605737123	21.38187640880639
17	20.90048573040065	29.972072487958247	27.912462608115398	21.21497917352571
18	20.881097287246398	29.861665515515394	28.599186039355036	20.658051157883172
19	21.473675246146822	28.750800596307734	28.817164873088245	20.958359284457188
20	22.50258532909271	28.481096827935183	28.087800400464115	20.928517442507992
21	22.920547901684106	28.830630037515785	27.192965812184553	21.05585624861555
22	22.07231545022827	28.292331584233132	28.551741506602017	21.083611458936577
23	22.293477304613162	28.702311553091064	27.836071711350108	21.16813943094567
24	21.97674872570603	27.863304193694887	28.253339941641322	21.906607138957764
25	22.55779659005743	28.106176600459026	27.646574110956575	21.68945269852697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13325.0
1	13325.0
2	11549.0
3	9773.0
4	9773.0
5	9773.0
6	10196.5
7	10620.0
8	10620.0
9	10620.0
10	14654.5
11	18689.0
12	18689.0
13	18689.0
14	29615.5
15	40542.0
16	40542.0
17	40542.0
18	63794.5
19	87047.0
20	87047.0
21	87047.0
22	123835.5
23	160624.0
24	160624.0
25	160624.0
26	208916.0
27	257208.0
28	257208.0
29	257208.0
30	310991.0
31	364774.0
32	364774.0
33	364774.0
34	413069.5
35	461365.0
36	461365.0
37	461365.0
38	496638.0
39	531911.0
40	531911.0
41	531911.0
42	534779.0
43	537647.0
44	537647.0
45	537647.0
46	521455.5
47	505264.0
48	505264.0
49	505264.0
50	484276.0
51	463288.0
52	463288.0
53	463288.0
54	426003.0
55	388718.0
56	388718.0
57	388718.0
58	338645.5
59	288573.0
60	288573.0
61	288573.0
62	242402.0
63	196231.0
64	196231.0
65	196231.0
66	155058.5
67	113886.0
68	113886.0
69	113886.0
70	85697.0
71	57508.0
72	57508.0
73	57508.0
74	41688.5
75	25869.0
76	25869.0
77	25869.0
78	18062.5
79	10256.0
80	10256.0
81	10256.0
82	6952.0
83	3648.0
84	3648.0
85	3648.0
86	2359.0
87	1070.0
88	1070.0
89	1070.0
90	644.0
91	218.0
92	218.0
93	218.0
94	131.5
95	45.0
96	45.0
97	45.0
98	27.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14471951853386067
2	0.2820073753745514
3	0.34271393731195476
4	0.3708355210562282
5	0.37800333677212594
6	0.33787676106196246
7	0.3406031694937763
8	0.357203478897159
9	0.3443849618346794
10	0.37501308236303976
11	0.2941442903290775
12	0.3395038112551417
13	0.31087652272109634
14	0.32147433614153403
15	0.360633476601699
16	0.31063466390859673
17	0.360061810317609
18	0.32903792082334016
19	0.34187842505059246
20	0.3049399882324694
21	0.34335156509036285
22	0.2987176206017975
23	0.28053423533478095
24	0.31707690318699555
25	0.31199786812450364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4548108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.77282551712406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.53066178167533	64.80899911547037
2	10.054307814753194	15.236866234855015
3	2.2539940516437165	5.123744939755046
4	0.831737740273005	2.5209247467885385
5	0.38902735118706144	1.4738850801443077
6	0.23424790606863555	1.0649775428574233
7	0.1464925431573483	0.7770107738555063
8	0.10803110783056756	0.6548657827253769
9	0.07507090602108385	0.5119501197013204
>10	0.34819547785211963	4.531447039192441
>50	0.018553629176682723	0.9608958061391778
>100	0.008660155419164432	1.0522757609202058
>500	6.117212802816211E-4	0.32267500135345745
>1k	3.4955456724224603E-4	0.721918095548703
>5k	5.8259094540332484E-5	0.23756396069307623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5656	0.12435940395434761	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5107	0.11228845049413953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4874	0.10716544110210223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1987164772692294E-5	0.0
3	0.0	0.0	0.0	4.397432954538459E-5	0.0
4	0.0	0.0	0.0	4.397432954538459E-5	0.0
5	0.0	0.0	0.0	4.397432954538459E-5	0.0
6	0.0	0.0	0.0	4.397432954538459E-5	0.0
7	0.0	0.0	0.0	6.596149431807688E-5	0.0
8	0.0	0.0	0.0	6.596149431807688E-5	0.0
9	0.0	0.0	0.0	1.0993582386346147E-4	0.0
10	0.0	2.1987164772692294E-5	0.0	1.0993582386346147E-4	0.0
11	0.0	2.1987164772692294E-5	0.0	1.0993582386346147E-4	0.0
12	0.0	2.1987164772692294E-5	0.0	1.3192298863615377E-4	0.0
13	0.0	2.1987164772692294E-5	0.0	1.3192298863615377E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2985	0.0	11.494956	1
ACCGTCC	105	3.473584E-6	10.85631	8
TGGTATC	1255	0.0	10.67791	2
GTGGTAT	1390	0.0	10.598914	1
CCGTCCA	160	6.193659E-9	10.092864	9
CATACCG	95	1.638074E-4	10.004424	5
CTAGGAC	410	0.0	9.272495	3
GTCCTAA	370	0.0	9.247945	1
GTAGACG	95	0.0018169845	9.00428	4
GTCTTAG	455	0.0	8.773691	1
AACAGCG	200	2.5460395E-8	8.549251	7
ATACCGT	170	1.616052E-6	8.381433	6
CCAGTAC	445	0.0	8.3296175	3
ACACCGT	160	6.465876E-6	8.311587	6
GTACTAA	390	0.0	8.04255	1
TATTCCG	225	2.1698725E-8	8.025771	5
GTATCAA	5280	0.0	8.010701	1
GTGTAGG	470	0.0	7.886988	1
CTATACT	640	0.0	7.8709283	4
TGTACCG	145	1.5553976E-4	7.8655467	5
>>END_MODULE