FastQCFastQC Report
Wed 25 May 2016
SRR1295200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4548108
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT148640.32681721718129825No Hit
GGTATCAACGCAGAGTACTTTTTTT129610.28497564261886477No Hit
TATCAACGCAGAGTACTTTTTTTTT89780.19740076532923143No Hit
GTGGTATCAACGCAGAGTACATGGG65300.14357618596568067No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60280.13253862924978912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.012.2294661
TGGTATC15050.011.425542
AGCGTCA3600.011.3475113
GTGGTAT15550.011.1209661
GTCTACG1451.6153353E-79.82934951
CCCTTAC3750.09.6283941
CGCAGTA1002.7567556E-49.5002412
TAGGACG1304.242036E-69.5002414
CAGCGTC4350.09.3910442
TCTATAC5500.09.15477853
TAGCGAG950.00182407729.0004275
GTCTCGC2252.6193447E-108.86825751
AGGACCG1304.370175E-58.7696475
TGCACCG1304.370175E-58.7696475
GTATTAG6000.08.7098961
GTATCAA76450.08.6006271
TCCCGTA1000.00290595978.5502182
GCAGCGT4400.08.4219661
GTATTAT7800.08.40535451
TAGACCC3200.08.3127124
Produced by FastQC (version 0.11.5)