FastQCFastQC Report
Wed 25 May 2016
SRR1295199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5536512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG66200.11956986637074028No Hit
GTATCAACGCAGAGTACTTTTTTTT63690.11503632612012762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN62060.11209223424423174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36000.010.7207971
AGACCGT1701.5661499E-910.0579166
CGTATAC1054.0840285E-59.9579853
GTGGTAT17250.09.8092191
GTACCGT1651.0257281E-89.7869966
CTAGACT4500.09.7168444
TGGTATC16850.09.5332962
ACCGCGT1201.7041535E-59.4991438
CCGCGTG1002.7604497E-49.4990569
ATTAGAC4000.09.26771453
GTATTAG7400.09.2492031
TGTACCG2304.1836756E-119.0921555
GTCTTAG4600.08.6795051
TAGACAG7000.08.5548935
GTCGTTA1000.00291159388.54845312
TACTCCG1901.0036092E-78.5048635
GTATCAA65000.08.4531381
GTATTAA9600.08.317861
TAGGACG1606.4064134E-68.3174074
GTCCTAG5750.08.2661951
Produced by FastQC (version 0.11.5)