FastQCFastQC Report
Wed 25 May 2016
SRR1295199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5536512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT178730.3228205772876497No Hit
GGTATCAACGCAGAGTACTTTTTTT144130.26032635709992136No Hit
TATCAACGCAGAGTACTTTTTTTTT101390.18312973944606278No Hit
GTGGTATCAACGCAGAGTACATGGG73750.13320661094927636No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63990.11557818352059925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT453.54146E-516.888936
AGCGTCA4300.012.5941923
GGTATCA54650.011.8070541
GTGGTAT21000.011.72041
GCAGCGT5150.010.8869621
TGGTATC22550.010.5752592
CAGCGTC5550.010.1000362
CCGTGCG800.00451979279.499689
TAGCACT5950.09.2612784
TACACCG1856.699338E-99.2442675
GCTCTAG3950.09.1421531
CTAGCAC4500.09.0786193
CTAGGAC5300.08.78383353
GTATTAA9200.08.7799711
GGCTACG1752.6166344E-78.6884811
TCTATAC5750.08.5921013
AGTACCG1000.00290392048.5509485
TTACGCT1000.00290463288.5507154
CACACCG2409.331416E-108.313425
CAATACT6650.08.28640654
Produced by FastQC (version 0.11.5)