FastQCFastQC Report
Wed 25 May 2016
SRR1295198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5065888
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG68470.13515892968814155No Hit
GTATCAACGCAGAGTACTTTTTTTT65140.12858555104258126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN58380.11524139499333583No Hit
GGTATCAACGCAGAGTACTTTTTTT54140.10687168764883866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.010.7272921
TAGACCG1106.0127622E-610.3693975
GTAGGAC2750.010.023753
CTAGGAC3650.09.8959093
TGGTATC18200.09.870772
GTGGTAT19800.09.8426921
CTTAGAC3250.09.6515163
TAGCACG950.00181507729.005364
GTCTAAG3950.08.9049171
TAGGACC3500.08.69088654
GTCTTAG3950.08.6642441
TAACGCT1000.00289111988.5550924
TAGGACT5050.08.2821584
CCAGTAC4950.08.25711253
GTATTAA10050.08.2295941
CGAATAG1050.00452069668.14181210
GTCCTGG7850.08.1139141
TTAGACT5450.08.0231244
GGACTGT6400.08.01435856
CGCGATC1553.914233E-57.96569417
Produced by FastQC (version 0.11.5)