FastQCFastQC Report
Wed 25 May 2016
SRR1295198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5065888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167870.33137329526432485No Hit
GGTATCAACGCAGAGTACTTTTTTT145440.2870967538169024No Hit
TATCAACGCAGAGTACTTTTTTTTT95280.18808153674143605No Hit
GTGGTATCAACGCAGAGTACATGGG73040.144180052934451No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62340.12305838581508316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51000.011.9253791
AGCGTCA4750.011.8006253
TAGACCG658.024683E-411.6930435
GCAGCGT4850.011.3644561
CGGTCGT855.3284766E-511.176410
GTGGTAT19500.011.0137761
CAGCGTC5400.010.5560112
TGGTATC19550.010.3022352
CTACACT6150.09.8867034
GTCTAAG4700.09.5030361
TAACGGT900.00111388859.5005044
TGGACCG1554.262729E-79.1941265
TACACCG2002.6575435E-99.0255685
CCGGTCG850.00743998078.941129
GCGTCAG6450.08.5430884
GCACCGT1356.807038E-58.4441416
CTAGTAC4000.08.3129413
ACCCGAC1150.00111139978.26081857
TACACTG8850.08.1587055
AGGACAG9800.08.143375
Produced by FastQC (version 0.11.5)