FastQCFastQC Report
Wed 25 May 2016
SRR1295197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847222
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG56440.1467032575713073No Hit
GTATCAACGCAGAGTACTTTTTTTT51000.1325631845523861No Hit
GGTATCAACGCAGAGTACTTTTTTT44050.1144982015594629No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN43440.11291264190109124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11850.012.1926081
GGTATCA28550.011.5862921
TGGTATC12300.011.049432
GCGACCA750.002651472610.1322349
ACACCGT1554.2736247E-79.192316
TTAGACT3000.09.1879734
CTGACGA1151.1053211E-49.0915474
TTCGCGG950.00182696488.99878814
AGCCTCG4900.08.9221742
TACACCG1201.7558222E-48.7125055
CAGGACT5650.08.5795544
CCTATAC3000.08.553653
GTCGTAG1356.7576264E-58.4492641
GTGTTAG4750.08.4047931
GTATCAA50800.08.4014451
CCTACAC4550.08.3552133
ACAACGC1150.00111237268.2599738
TATTCCG1401.034024E-48.1467595
CTTCGCG1050.0045199218.14186713
CGGGCGA1050.00453002478.13984818
Produced by FastQC (version 0.11.5)