FastQCFastQC Report
Wed 25 May 2016
SRR1295197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847222
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134380.349290994904895No Hit
GGTATCAACGCAGAGTACTTTTTTT118330.30757258094282053No Hit
TATCAACGCAGAGTACTTTTTTTTT78300.20352347745983984No Hit
GTGGTATCAACGCAGAGTACATGGG60310.15676246392851775No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50800.1320433289266905No Hit
GGTATCAACGCAGAGTACATGGGGG39300.10215163044919166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14550.012.4086051
GGTATCA41050.012.3843431
TGGTATC14150.012.2203942
CTAGTAC1405.2750693E-1112.2157573
GGACCGT1201.0029908E-811.875046
CGATACC600.005875339311.0840914
AGCGTCA3000.010.7679633
ACCGGGC951.6447662E-410.0002958
GCGAACC900.00111386799.50040211
ACCGTCA900.00111400039.5002798
GGTCTAG1752.6626367E-89.2308831
ATAGGAC3250.09.062633
CTAGACA3050.09.0334054
TAACCCG950.00182338019.0007325
GACCGTG1804.2231477E-88.9723697
TATAACG850.00743236638.9421382
TGCGCAA850.00743712258.9414410
TAGACAG5850.08.7699455
AGGACCG1304.367886E-58.7699455
TACCGAG1201.7646572E-48.7084767
Produced by FastQC (version 0.11.5)