FastQCFastQC Report
Wed 25 May 2016
SRR1295196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704009
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG73890.15707878109927084No Hit
GTATCAACGCAGAGTACTTTTTTTT62510.1328866505144867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54920.11675147730372114No Hit
GGTATCAACGCAGAGTACTTTTTTT51300.10905591379608329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG1101.8553692E-1013.8227093
GGTATCA36900.011.6170241
GTGGTAT15650.011.4179521
TGGTATC15650.010.6872062
GCGTAGC1303.8297912E-710.2341212
ACCGTTT900.00111573559.4987528
CGTGTCG800.00452481079.4983469
TCTGTCG4650.09.1923418
GTACTAG2800.09.165381
AGACCGT1356.8699355E-69.1465556
GTATTAG6400.08.9107861
GTACCGG1502.71122E-68.8651236
CTAGACT4300.08.84019854
GTCTTAG4550.08.7736961
ACAGCGC2304.0563464E-108.6727728
CTGTCGC5400.08.6188699
CCTACTC3750.08.6161563
TCTAGAC4150.08.4726543
AGTACCG1356.778687E-58.4471225
AACACCG1356.778687E-58.4471225
Produced by FastQC (version 0.11.5)