FastQCFastQC Report
Wed 25 May 2016
SRR1295196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704009
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164620.3499568134329675No Hit
GGTATCAACGCAGAGTACTTTTTTT140330.2983200074659721No Hit
TATCAACGCAGAGTACTTTTTTTTT93220.19817138955303867No Hit
GTGGTATCAACGCAGAGTACATGGG76250.16209577830314525No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60300.1281885302515365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19100.012.8869731
TGGTATC19100.012.3362392
GGTATCA51450.012.1541621
AGCGTCA4700.012.1289283
CAGCGTC5550.010.4424232
GCAGCGT5400.09.8555021
TGTACCG1304.2373013E-69.5010955
CGTGTTA1752.6770067E-89.22796710
TAGGACG1554.259855E-79.19450954
GAACCGT1356.854947E-69.1481336
TAACACG1401.0847973E-58.8223514
GTATAGG4900.08.7277221
AATCGTG1000.0029064318.55007657
ACCGTTC1000.00290810958.5495318
ACACCGC1451.690847E-58.5172276
GTGTAGG5150.08.3040471
TACACCG1150.00111002718.2618225
GTATTAG5100.08.1991151
CTAGACA5150.08.1173544
TATACTG8200.08.1106915
Produced by FastQC (version 0.11.5)