FastQCFastQC Report
Wed 25 May 2016
SRR1295195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625970
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA397772.446355098802561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA329432.026052141183417No Hit
GCTTATGTACTCTGCGTTGATACCA232161.4278246216104848No Hit
CTTATGTACTCTGCGTTGATACCAC162020.9964513490408802No Hit
GTATCAACGCAGAGTACTTTTTTTT109400.6728291419890896No Hit
GAGTACATAAGCAGTGGTATCAACG105810.6507500138378937No Hit
TATGTACTCTGCGTTGATACCACTG92580.5693831989520102No Hit
CCCATGTACTCTGCGTTGATACCAC87260.5366642680984274No Hit
GGTATCAACGCAGAGTACTTTTTTT79450.4886314015633744No Hit
GCGTTGATACCACTGCTTATGTACT77020.473686476380253No Hit
TATCAACGCAGAGTACTTTTTTTTT70250.4320497918165772No Hit
CATAAGCAGTGGTATCAACGCAGAG69380.42669913959052136No Hit
GTGGTATCAACGCAGAGTACATGGG63520.39065911425180044No Hit
ACTCTGCGTTGATACCACTGCTTAT62910.38690750751858893No Hit
ACGCAGAGTACATAAGCAGTGGTAT61320.3771287293123489No Hit
GTACTGGTTCACTATCGGTCAGTCA60190.37017903159344884No Hit
CTGCTTATGTACTCTGCGTTGATAC59490.3658739091127143No Hit
CATGTACTCTGCGTTGATACCACTG55810.34324126521399534No Hit
ATCATGTACTCTGCGTTGATACCAC51550.3170415198312392No Hit
GTACATGATAAGCAGTGGTATCAAC51340.31574998308701885No Hit
GTATCAACGCAGAGTACATAAGCAG50500.31058383611013735No Hit
ATACAGGGTGACAGCCCCGTACACA50400.30996881861288955No Hit
TATCAACGCAGAGTACATAAGCAGT47170.2901037534517857No Hit
GGATACCACGTGTCCCGCCCTACTC45260.2783569192543528No Hit
GGTATCAACGCAGAGTACATAAGCA44580.2741748002730678No Hit
GTACATGGGAAGCAGTGGTATCAAC38580.23727375043819995No Hit
ACCCTGTATCGCGCGCCTTTCCAGA36630.22528090924186792No Hit
AAGCAGTGGTATCAACGCAGAGTAC36360.22362036199929888No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34160.2100899770598474No Hit
GTGGTATCAACGCAGAGTACATAAG31850.19588307287342327No Hit
ACATAAGCAGTGGTATCAACGCAGA31490.1936690098833312No Hit
ATCATTAACTGAATCCATAGGTTAA31200.19188545914131258No Hit
TCTAAGTACCCCGAGGAAAAGAAAT31030.19083992939599131No Hit
GGTTAATGAGGCGAACCGGGGGAAC30440.18721132616222932No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29950.1841977404257151No Hit
CCCATATTCAGACAGGATACCACGT29240.17983111619525577No Hit
GGTATCAACGCAGAGTACATGGGGG28940.1779860637035124No Hit
GCAGTGGTATCAACGCAGAGTACAT26460.162733629771767No Hit
GTACTCTGCGTTGATACCACTGCTT26280.161626598276721No Hit
GTATTTAGCCTTGGAGGATGGTCCC25910.1593510335369041No Hit
CCACTGCTTATGTACTCTGCGTTGA24100.148219216836719No Hit
CATCTAAGTACCCCGAGGAAAAGAA23820.14649716784442518No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA22990.14139252261726848No Hit
GATACCACTGCTTATGTACTCTGCG22810.1402854911222224No Hit
TATTCAGACAGGATACCACGTGTCC22760.13997798237359854No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG22760.13997798237359854No Hit
CACACACACTGATTCAGGCTCTGGG22720.1397319753746994No Hit
GCCTTGGAGGATGGTCCCCCCATAT22470.13819443163157993No Hit
GTTCACTATCGGTCAGTCAGGAGTA21890.1346273301475427No Hit
GTACATGGGATAAGCAGTGGTATCA21730.13364330215194622No Hit
ATACCACTGCTTATGTACTCTGCGT21470.13204425665910197No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21000.12915367442203732No Hit
GTTGATACCACTGCTTATGTACTCT20360.1252175624396514No Hit
GAGTACATGATAAGCAGTGGTATCA19730.12134295220699029No Hit
GTTAATGATAGTGTGTCGAAACACA19560.12029742246166904No Hit
GCTTATCCCATGTACTCTGCGTTGA19500.11992841196332037No Hit
CTATCGGTCAGTCAGGAGTATTTAG18890.1161768052301088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN18610.11445475623781495No Hit
TCACGGTACTGGTTCACTATCGGTC18610.11445475623781495No Hit
GAGTAAGCAGTGGTATCAACGCAGA18570.11420874923891584No Hit
CGGTACTGGTTCACTATCGGTCAGT18230.11211768974827332No Hit
GTCAGGAGTATTTAGCCTTGGAGGA18090.11125666525212642No Hit
GCTTATCATGTACTCTGCGTTGATA17840.10971912150900694No Hit
CCCCAGTAGCGGCGAGCGAACGGGG17700.10885809701286No Hit
GGTATCAACGCAGAGTACATGGGAA17170.10559850427744669No Hit
GAGTACATGGGAAGCAGTGGTATCA17060.10492198503047412No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16830.1035074447868042No Hit
CAGTGGTATCAACGCAGAGTACATA16470.10129338179671213No Hit
TTCCAGACGCTTCCACTAACACACA16460.10123188004698734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT250.006037111418.99731416
GTCTTAG402.753787E-416.6308651
ACGTAAT402.7628848E-416.62367612
GTAACCG402.7628848E-416.6236769
CACGTAA402.7635356E-416.62316311
CACGGGC350.00217140716.28441812
TCCCGGT551.129219E-515.54421612
GTACAAA2000.015.2053631
TAGGGGT456.7444827E-414.7829914
GCGTTAC456.766629E-414.77660215
CGTAATA456.766629E-414.77660213
GGACCGT456.766629E-414.7766026
ACGCGAT653.3762044E-614.613770511
AGCGCCA851.7234925E-814.52511619
GTCTTCG400.0052668914.2550281
GTAAGAC1006.566552E-1014.2545873
GATACAC400.005267969314.2545863
TTCGGTA400.005267969314.2545862
TCTATAG400.005267969314.2545862
CGGGCAA400.005282019714.24886514
Produced by FastQC (version 0.11.5)