FastQCFastQC Report
Wed 25 May 2016
SRR1295195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625970
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA424442.610380265318548No Hit
GTATCAACGCAGAGTACTTTTTTTT308641.8981900035055996No Hit
GCTTATGTACTCTGCGTTGATACCA251641.5476300300743557No Hit
GGTATCAACGCAGAGTACTTTTTTT230811.4195218853976395No Hit
TATCAACGCAGAGTACTTTTTTTTT201041.2364311764669704No Hit
CTTATGTACTCTGCGTTGATACCAC190371.1708088095106306No Hit
GAGTACATAAGCAGTGGTATCAACG114180.7022269783575343No Hit
ACGCAGAGTACTTTTTTTTTTTTTT105720.6501964980903707No Hit
CCCATGTACTCTGCGTTGATACCAC101870.6265183244463305No Hit
GTACTTTTTTTTTTTTTTTTTTTTT97590.6001955755641248No Hit
GCGTTGATACCACTGCTTATGTACT86820.5339581911105371No Hit
CATAAGCAGTGGTATCAACGCAGAG85570.5262704723949396No Hit
TATGTACTCTGCGTTGATACCACTG84620.5204278061710855No Hit
ACTCTGCGTTGATACCACTGCTTAT70370.4327878128132745No Hit
CTGCTTATGTACTCTGCGTTGATAC69580.4279291745850169No Hit
ACGCAGAGTACATAAGCAGTGGTAT67530.41532131589143717No Hit
GTGGTATCAACGCAGAGTACATGGG66020.4060345516829954No Hit
GTACTGGTTCACTATCGGTCAGTCA64110.39428771748556246No Hit
CATGTACTCTGCGTTGATACCACTG63940.3932421877402412No Hit
GAGTACTTTTTTTTTTTTTTTTTTT58490.3597237341402363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA58350.3588627096440894No Hit
ATACAGGGTGACAGCCCCGTACACA58030.35689465365289647No Hit
ATCATGTACTCTGCGTTGATACCAC54760.33678358149289345No Hit
GTATCAACGCAGAGTACATAAGCAG53960.3318634415149111No Hit
GTACATGATAAGCAGTGGTATCAAC53910.3315559327662872No Hit
TATCAACGCAGAGTACATAAGCAGT51410.3161804953350923No Hit
GGATACCACGTGTCCCGCCCTACTC49510.30449516288738415No Hit
GGTATCAACGCAGAGTACATAAGCA47500.2921333111927034No Hit
GTACATGGGAAGCAGTGGTATCAAC41190.2533257071163674No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41060.25252618436994534No Hit
AAGCAGTGGTATCAACGCAGAGTAC40800.25092713887710105No Hit
ACCCTGTATCGCGCGCCTTTCCAGA40550.2493895951339816No Hit
ACATAAGCAGTGGTATCAACGCAGA35340.21734718352737137No Hit
CCCATATTCAGACAGGATACCACGT33920.20861393506645265No Hit
GTGGTATCAACGCAGAGTACTTTTT33190.20412430733654374No Hit
GTGGTATCAACGCAGAGTACATAAG32660.2008647146011304No Hit
ATCATTAACTGAATCCATAGGTTAA31700.19496054662755155No Hit
GGTATCAACGCAGAGTACATGGGGG30810.18948689090204615No Hit
TCTAAGTACCCCGAGGAAAAGAAAT30610.1882568559075506No Hit
GGTTAATGAGGCGAACCGGGGGAAC30140.18536627367048591No Hit
CACACACACTGATTCAGGCTCTGGG29240.17983111619525577No Hit
ATCAACGCAGAGTACTTTTTTTTTT29230.17976961444553097No Hit
GCAGTGGTATCAACGCAGAGTACAT28240.1736809412227778No Hit
CCACTGCTTATGTACTCTGCGTTGA27280.16777677324919893No Hit
CATCTAAGTACCCCGAGGAAAAGAA26620.16371765776736347No Hit
GTACTCTGCGTTGATACCACTGCTT26010.15996605103415193No Hit
GATACCACTGCTTATGTACTCTGCG25340.1558454338025917No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG24860.15289334981580224No Hit
GCCTTGGAGGATGGTCCCCCCATAT24730.15209382706938013No Hit
CTATCGGTCAGTCAGGAGTATTTAG24540.1509252938246093No Hit
GTATTTAGCCTTGGAGGATGGTCCC24210.14889573608369158No Hit
GCCCAGAGCCTGAATCAGTGTGTGT23980.14748119584002164No Hit
GTACATGGGATAAGCAGTGGTATCA23300.14329907685873663No Hit
ATACCACTGCTTATGTACTCTGCGT22790.14016248762277286No Hit
GAGTACATGATAAGCAGTGGTATCA22130.1361033721409374No Hit
GTTCACTATCGGTCAGTCAGGAGTA21990.13524234764479048No Hit
TATTCAGACAGGATACCACGTGTCC21970.13511934414534094No Hit
CGGTACTGGTTCACTATCGGTCAGT21740.133704803901671No Hit
CTTATACACATCTCCGAGCCCACGA21470.13204425665910197No Hit
GCTTATCCCATGTACTCTGCGTTGA21380.13149074091157895No Hit
GAGTAAGCAGTGGTATCAACGCAGA21350.1313062356624046No Hit
GTCAGGAGTATTTAGCCTTGGAGGA20770.12773913417836738No Hit
GTTGATACCACTGCTTATGTACTCT20700.1273086219302939No Hit
CCCCAGTAGCGGCGAGCGAACGGGG20340.12509455894020186No Hit
GTTAATGATAGTGTGTCGAAACACA20110.12368001869653192No Hit
TCACGGTACTGGTTCACTATCGGTC19170.11789885422240262No Hit
GCCTGAATCAGTGTGTGTGTTAGTG19160.11783735247267785No Hit
GCTTATCATGTACTCTGCGTTGATA19030.11703782972625572No Hit
GAGTACATGGGAAGCAGTGGTATCA18830.11580779473176013No Hit
CAGTGGTATCAACGCAGAGTACATA18730.11519277723451232No Hit
GGTATCAACGCAGAGTACATGGGAA17780.10935011101065825No Hit
TACCACTGCTTATGTACTCTGCGTT17580.10812007601616266No Hit
GATAAGCAGTGGTATCAACGCAGAG17540.10787406901726353No Hit
GATTCAGGCTCTGGGCTGCTCCCCG17500.10762806201836442No Hit
GAATCAGTGTGTGTGTTAGTGGAAG17160.1055370025277219No Hit
GCAGAGTACATAAGCAGTGGTATCA16850.10363044828625376No Hit
CAGTAGCGGCGAGCGAACGGGGAGC16310.10030935380111565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2400.019.0048921
AGGCGTA555.907459E-717.27345514
ACTAGAC453.537772E-516.8895993
GTCTAGC402.755798E-416.629281
CGCAAAA402.7603286E-416.62572
TCCGGAC350.002172770416.28289217
CCCGAAC350.002172770416.28289216
ACGCGAT601.4662692E-615.83400111
AACGCGA601.4662692E-615.83400110
TTCTACT551.1279219E-515.5461092
CACCTAG508.71121E-515.200647
AGCGCCA704.4435E-714.92644419
TATACAC3450.014.8701913
CAAAGCG456.769875E-414.77567319
GGTAACC653.3708839E-614.6159998
CGTAATA653.3708839E-614.61599913
TTATACA3150.014.4768012
AATCGCC1058.913048E-1114.47368418
CATATAC400.00527023314.2536691
ACGTAAT602.567385E-514.25060112
Produced by FastQC (version 0.11.5)