FastQCFastQC Report
Wed 25 May 2016
SRR1295194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG73430.14441897275490745No Hit
GTATCAACGCAGAGTACTTTTTTTT67370.13250042481953037No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN63160.12422037749148787No Hit
GGTATCAACGCAGAGTACTTTTTTT57570.11322620538608229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.011.2008841
GTGGTAT17950.010.2219291
TGGTATC17400.09.9424512
GTCCTAA4200.09.7332781
TCTAGAC4350.09.1781013
CGGTCGT850.00744940348.93974110
GTACCGT1502.7087372E-68.8657686
GTATAGG5700.08.6729821
TAGGACG1451.6769305E-58.5228594
GTATTAT8050.08.5030871
CTAGGAC4650.08.3815373
ACACCGC1252.7484845E-48.3591536
GCACCGT1606.465765E-68.3116586
CTGTCGC4250.08.2698329
GTATCAA72950.08.1971961
GTCGTAG1401.031226E-48.1487911
CCGGTCG1304.1929164E-48.0377269
GTCCTAG5000.07.98581551
CTAGTAC2502.05182E-97.98494773
TGTCGCC4300.07.95221110
Produced by FastQC (version 0.11.5)