##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295194_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5084512
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.36114203290306	31.0	30.0	33.0	26.0	34.0
2	30.400095427053767	31.0	30.0	34.0	26.0	34.0
3	30.33090078261198	31.0	30.0	34.0	26.0	34.0
4	33.41747683946857	35.0	33.0	37.0	27.0	37.0
5	33.88027995607052	35.0	35.0	37.0	28.0	37.0
6	34.084383516058175	37.0	35.0	37.0	29.0	37.0
7	33.994516484571186	37.0	35.0	37.0	28.0	37.0
8	34.00771263790901	37.0	35.0	37.0	28.0	37.0
9	35.01923036075045	39.0	35.0	39.0	27.0	39.0
10	34.99737221585867	38.0	35.0	39.0	27.0	39.0
11	35.50900243720538	39.0	35.0	39.0	28.0	39.0
12	35.48189796778924	39.0	35.0	39.0	28.0	39.0
13	35.45153713866739	39.0	35.0	39.0	27.0	39.0
14	36.15184387410237	39.0	36.0	41.0	27.0	41.0
15	36.51769668357553	40.0	36.0	41.0	27.0	41.0
16	36.52121383527072	40.0	36.0	41.0	27.0	41.0
17	36.22243098256037	40.0	36.0	41.0	27.0	41.0
18	36.12411004241902	39.0	36.0	41.0	27.0	41.0
19	36.52145338628368	40.0	36.0	41.0	27.0	41.0
20	36.50967310137138	40.0	36.0	41.0	27.0	41.0
21	36.55994341246515	40.0	36.0	41.0	27.0	41.0
22	36.486388074214396	40.0	36.0	41.0	27.0	41.0
23	36.22967297549893	40.0	36.0	41.0	27.0	41.0
24	36.48041994983983	40.0	36.0	41.0	27.0	41.0
25	35.283697825868046	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.16139328157545307
1101	2	0.10341241769625142
1101	3	-0.1732471325281537
1101	4	-0.1767917911108725
1101	5	-0.2968346364777972
1101	6	-0.2874747286594541
1101	7	-0.25844872959684295
1101	8	-0.23885678124928944
1101	9	2.2924559462467187E-4
1101	10	0.03626444437727372
1101	11	-0.04678646888412885
1101	12	0.029226924813535504
1101	13	0.12264532439238707
1101	14	0.3911253363866578
1101	15	0.2204675939461751
1101	16	0.051750505699359906
1101	17	0.14440490819321639
1101	18	0.3567593755828966
1101	19	0.1609827777119648
1101	20	0.10789980392090825
1101	21	0.12952641845689783
1101	22	0.04386663637195909
1101	23	0.16341157187721222
1101	24	-0.019939213174147596
1101	25	-0.035207112683970365
1102	1	-0.2540959842781554
1102	2	-0.006214609330776
1102	3	-0.24261109649211576
1102	4	-0.4615881875072674
1102	5	-0.6407049067480699
1102	6	-0.5108350890198139
1102	7	-0.5689622431103558
1102	8	-0.5917901145826221
1102	9	-0.38669147512609925
1102	10	-0.05734816823533606
1102	11	-0.11125673915439904
1102	12	-0.2438109130243049
1102	13	-0.22930422515716486
1102	14	-0.006418807757491152
1102	15	-0.10546754118896473
1102	16	-0.22397562042677066
1102	17	-0.24562572243741698
1102	18	0.12500442062794548
1102	19	-0.2223757808465976
1102	20	-0.14221911499800655
1102	21	-0.11983574370525929
1102	22	-0.24996039065506892
1102	23	-0.2457650046993578
1102	24	-0.22674461857955208
1102	25	-0.35977648205334134
1103	1	-0.5304882038358443
1103	2	-0.26898497970114477
1103	3	-0.5622803744031231
1103	4	-0.6624341240913338
1103	5	-0.7128203344059472
1103	6	-0.7823901116683629
1103	7	-0.7352889151060964
1103	8	-0.6456646682730067
1103	9	-0.33335718256014957
1103	10	-0.22103375310077666
1103	11	-0.29796134880001546
1103	12	-0.5088613793179348
1103	13	-0.383626847059638
1103	14	-0.38716615654512765
1103	15	-0.38863503386149034
1103	16	-0.4772000275677186
1103	17	-0.2685105612330432
1103	18	-0.04119584582556257
1103	19	-0.2872293912915751
1103	20	-0.2626764557920396
1103	21	-0.334269103683944
1103	22	-0.2496218881237624
1103	23	-2.5628199474118603
1103	24	-1.2631770193256244
1103	25	-0.78922609569576
1104	1	-0.38106227641298673
1104	2	-0.10185320814108678
1104	3	-0.40199518990476335
1104	4	-0.753474594204782
1104	5	-0.6117645234037212
1104	6	-0.7267852983170187
1104	7	-0.5436509410614505
1104	8	-0.5349241969796239
1104	9	-2.734449395372849
1104	10	-0.9496054936374065
1104	11	-0.6683026082885632
1104	12	-0.5262844093891417
1104	13	-0.4001670583803403
1104	14	-0.18323801903750336
1104	15	-0.3781176403297408
1104	16	-0.35291714939790353
1104	17	-0.055083400340038224
1104	18	0.013670470418645664
1104	19	-0.2799309251515112
1104	20	-0.34265824255914623
1104	21	-0.313422260559193
1104	22	-0.3613692472314085
1104	23	-0.32163933825300717
1104	24	-0.3495250547989812
1104	25	-0.3695621617184628
1105	1	-0.21297485530912397
1105	2	-0.1827515490929592
1105	3	-0.23544512236432524
1105	4	-0.08154308101308061
1105	5	-0.1433978637583948
1105	6	-0.24844726725057598
1105	7	-0.2765833067455361
1105	8	-0.30428863803747674
1105	9	-2.257024207387957
1105	10	-0.6880043814850438
1105	11	-0.38073568858599316
1105	12	-0.25536904708937413
1105	13	-0.19785417942346584
1105	14	-0.10542374699700474
1105	15	-0.09287216682596977
1105	16	-0.2075652192768942
1105	17	-1.3088985304865446
1105	18	-0.17255596092177683
1105	19	-0.08591630090408842
1105	20	-0.1854565904978145
1105	21	-0.1975423162911767
1105	22	-0.11722938320701104
1105	23	-0.11970557702394302
1105	24	-0.08226151391471603
1105	25	-0.5892217192152387
1106	1	-0.2568191925155219
1106	2	-0.3623051661658927
1106	3	-0.39995033688411397
1106	4	-0.36633455430490613
1106	5	-0.23577988303854625
1106	6	-0.34577732810214457
1106	7	-0.3609210562793024
1106	8	-0.42633137818737055
1106	9	-3.130107832439922
1106	10	-0.8642113755694822
1106	11	-0.6711137774960818
1106	12	-0.4311661223455303
1106	13	-0.4585837627085354
1106	14	-0.667082168639034
1106	15	-0.6661375317051537
1106	16	-0.6419726036720377
1106	17	-0.4951163424979299
1106	18	-0.051565944724536905
1106	19	-0.25468502530308257
1106	20	-0.33451051182770897
1106	21	-0.2613730421743057
1106	22	-0.3687869995156561
1106	23	-0.12405806345382331
1106	24	-0.33770676463022653
1106	25	-0.47167624270004893
1107	1	-0.22049325666103314
1107	2	-0.03392209765000587
1107	3	-0.11223593127765596
1107	4	-1.428067170543848
1107	5	-0.4128568956355565
1107	6	-0.2369231472763289
1107	7	-0.1065240564706329
1107	8	-0.1176609231863992
1107	9	0.30601166079173936
1107	10	0.44527303814594177
1107	11	0.14370787759014547
1107	12	-0.4293862634473342
1107	13	-0.14027375834738365
1107	14	0.1958638373576349
1107	15	-0.45430638447725613
1107	16	-0.8535859584324115
1107	17	-0.04663465454163429
1107	18	-0.10440511761341753
1107	19	0.07474690299395803
1107	20	-0.2591956524396082
1107	21	-0.06656553282005007
1107	22	0.011701334310203038
1107	23	0.31313903200560844
1107	24	-0.07951244279978198
1107	25	-0.2896297295172445
1108	1	0.4683489550932869
1108	2	0.441771433727876
1108	3	0.45320093023394037
1108	4	0.8846984742101256
1108	5	0.652298706219014
1108	6	0.48590779640458237
1108	7	0.5116544359294863
1108	8	0.559398601081817
1108	9	0.8922912519216624
1108	10	0.9710448038012842
1108	11	0.6520115607713635
1108	12	0.6046828261491726
1108	13	0.5037416545755775
1108	14	0.6708527586864577
1108	15	0.6416056585140808
1108	16	0.6577203954108981
1108	17	0.7220754406410279
1108	18	0.9372510922438764
1108	19	0.6593680449391002
1108	20	0.6390452255117651
1108	21	0.6032439890341621
1108	22	0.6381189956354518
1108	23	0.8346610284607507
1108	24	0.6588313279683291
1108	25	0.6755447750612333
1109	1	0.4232900339589243
1109	2	0.25719086179384476
1109	3	0.3508385488979542
1109	4	0.7981593302232284
1109	5	0.5800987406683404
1109	6	0.5373371500885895
1109	7	0.42279459579541623
1109	8	0.4337572005136252
1109	9	0.896129598835401
1109	10	0.6190571002550342
1109	11	0.46897669549606036
1109	12	0.472240150805483
1109	13	0.3673409622795134
1109	14	0.7758920331746282
1109	15	0.5079464405739529
1109	16	0.5015311678204455
1109	17	0.8251775197105076
1109	18	0.5721590305570174
1109	19	0.3865680894674881
1109	20	0.43539425886217487
1109	21	0.41967428668987594
1109	22	0.42683960934493115
1109	23	0.6258978119166443
1109	24	0.44734535102531225
1109	25	0.4764062940358187
1110	1	0.35229517381891284
1110	2	0.2729375290642295
1110	3	0.29269680035234913
1110	4	0.5057177871843876
1110	5	0.5046202253999326
1110	6	0.4054189555100578
1110	7	0.33863122157008263
1110	8	0.40001875935618614
1110	9	0.9162400641979431
1110	10	0.8933530971819295
1110	11	0.519538647678857
1110	12	0.4763097981722382
1110	13	0.4732671668018327
1110	14	0.694665799002081
1110	15	0.48232909504361743
1110	16	0.493131988094774
1110	17	0.8635876685605055
1110	18	0.8923169947109173
1110	19	0.47800967486379875
1110	20	0.5570058729885332
1110	21	0.5240389319314502
1110	22	0.6024667116995701
1110	23	0.6304350273365174
1110	24	0.5176419263507697
1110	25	0.4829311264682019
1111	1	0.25257389291904886
1111	2	0.20504539548502265
1111	3	0.36805778544062306
1111	4	0.7673431552971266
1111	5	0.6620378815977048
1111	6	0.4543436572680051
1111	7	0.3719421633428084
1111	8	0.38304305019785545
1111	9	0.829853255590649
1111	10	0.6029587584843057
1111	11	0.4211240939782783
1111	12	0.2742111433205068
1111	13	0.27963402028789375
1111	14	0.3937132734689328
1111	15	0.299683530325602
1111	16	0.12761490850813573
1111	17	0.41635514280056896
1111	18	0.516360966097082
1111	19	0.3889151092729932
1111	20	-0.12008966640662777
1111	21	-0.21468004902671822
1111	22	-0.021814375726101787
1111	23	0.31887889930577273
1111	24	0.3272715624943672
1111	25	0.28121130187795274
1112	1	0.2599349870967842
1112	2	0.18667398757208886
1112	3	0.31608391367525357
1112	4	0.7815920408675154
1112	5	0.6444201988361655
1112	6	0.4591514587668257
1112	7	0.460273796307753
1112	8	0.46961342053391064
1112	9	-1.0597117160145686
1112	10	0.21824670115305622
1112	11	0.13881139787047658
1112	12	0.1832567547373145
1112	13	0.16163323769282556
1112	14	0.5808846804327175
1112	15	0.3066604738274634
1112	16	0.20955993408598061
1112	17	0.32319630165143565
1112	18	0.3597693823047763
1112	19	0.2723804212494443
1112	20	0.1871554509334814
1112	21	0.2958203801802384
1112	22	0.3711052123482119
1112	23	0.09582060456076391
1112	24	0.11834613693429219
1112	25	0.3345068903852635
1113	1	0.35730256058845455
1113	2	0.19059892571946335
1113	3	0.35162267954052595
1113	4	0.69293338157307
1113	5	0.691341796104453
1113	6	0.5356016049787229
1113	7	0.4300131582061937
1113	8	0.4268956151013299
1113	9	0.34832844249333306
1113	10	0.558500159131178
1113	11	0.4417485501399767
1113	12	0.22267214506554467
1113	13	0.26383296543451706
1113	14	0.011961944446383654
1113	15	0.22641110530601338
1113	16	0.392279497562221
1113	17	0.6431296688702801
1113	18	0.5623995624842024
1113	19	0.36256472806513074
1113	20	0.374255166096809
1113	21	0.31739428285669646
1113	22	0.33037581444944664
1113	23	0.10217953085131626
1113	24	0.21807205771101223
1113	25	0.15881382475652828
1114	1	0.37891207200233623
1114	2	0.277772546007931
1114	3	0.41303834561991337
1114	4	0.7480524040430936
1114	5	0.6387534842652087
1114	6	0.5228198147208687
1114	7	0.2902566380878753
1114	8	0.37549134084307667
1114	9	0.033173376040736
1114	10	0.4563567373964972
1114	11	0.3009467783765274
1114	12	0.2908058576553785
1114	13	0.3314058470846035
1114	14	0.6492405439577809
1114	15	0.36948087592212175
1114	16	0.25809156819610024
1114	17	0.5921756921395342
1114	18	0.4823089782889767
1114	19	0.307866558122349
1114	20	0.34125933728838476
1114	21	0.33788167374623157
1114	22	0.14192414355575522
1114	23	0.5178359786963824
1114	24	0.3262059317438357
1114	25	0.3187235671164501
1115	1	0.281426471149512
1115	2	0.20689204830858543
1115	3	0.41295929393893616
1115	4	0.8156901695227461
1115	5	0.6291284491535336
1115	6	0.48247522467912063
1115	7	0.5595983526919426
1115	8	0.6132646360380036
1115	9	0.7423171459526614
1115	10	0.4362595952492043
1115	11	0.3528721707982143
1115	12	0.42359852051214375
1115	13	0.3197308459328809
1115	14	0.38492183769500343
1115	15	0.2997084461728079
1115	16	0.3531576677700272
1115	17	0.6207016077363576
1115	18	0.7880242355681375
1115	19	0.5241145825025342
1115	20	0.6235577299579802
1115	21	0.40387745873211145
1115	22	-0.1954849223634909
1115	23	0.35479143854144013
1115	24	0.3900706375703038
1115	25	0.37821972647746804
1116	1	0.5504311336061996
1116	2	0.4152580934930725
1116	3	0.7819454850109437
1116	4	1.1771270672364906
1116	5	0.9978928521044637
1116	6	0.8229500596429631
1116	7	0.778528568185024
1116	8	0.6870732949497267
1116	9	1.0927260655880815
1116	10	0.7317864500225468
1116	11	0.6287993791781901
1116	12	0.5371622828519875
1116	13	0.5114672076831184
1116	14	0.9240908590585875
1116	15	0.6488241665654044
1116	16	0.6710225567732948
1116	17	1.0722510443338393
1116	18	1.050395485920042
1116	19	0.7097049602261691
1116	20	0.6582144125681069
1116	21	0.7014252155058571
1116	22	0.6964406850957161
1116	23	0.9473153479078391
1116	24	0.6457468202371217
1116	25	0.697128284120275
1201	1	-0.21493654465173861
1201	2	0.1642939964063821
1201	3	-0.2597739161496655
1201	4	-0.3258143827485398
1201	5	-0.46310890941651905
1201	6	-0.4765798077211727
1201	7	-0.48023108900762423
1201	8	-0.5258814643713734
1201	9	-0.00376143287570585
1201	10	0.08723977928523396
1201	11	-0.03320417077547688
1201	12	0.07536507096502021
1201	13	-0.021015855132702654
1201	14	0.35565475239372546
1201	15	0.04797421998515006
1201	16	-0.026988473439885752
1201	17	0.07429573631286246
1201	18	0.2092271468898943
1201	19	-0.0461926365281613
1201	20	-0.008290629340905298
1201	21	-0.003743148641888183
1201	22	-0.05153263882051107
1201	23	-0.16159618103914397
1201	24	-0.1712118634094395
1201	25	-0.09486111625391658
1202	1	-0.38910924687762716
1202	2	-0.3527260151663185
1202	3	-0.6668706897724022
1202	4	-0.7718000354294645
1202	5	-1.014531962450988
1202	6	-0.8077776177554483
1202	7	-0.6963256383358214
1202	8	-0.7132599642731634
1202	9	-0.11924921164245461
1202	10	-0.003700069207930312
1202	11	-0.28301164631619713
1202	12	-0.3228284196556004
1202	13	-0.26391571941003633
1202	14	-0.36834595187773544
1202	15	-0.2889380982181393
1202	16	-0.23463203068317995
1202	17	-0.021818118547052734
1202	18	0.0321669316801092
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2211	11	0.006265400588738146
2211	12	-0.008111325419733362
2211	13	0.017855996358093762
2211	14	0.2628943535480346
2211	15	-0.012252082251762886
2211	16	0.07871734528549723
2211	17	0.23961556423632402
2211	18	0.3739005676763725
2211	19	0.022863714852711325
2211	20	0.22503249334415898
2211	21	0.12768356818350668
2211	22	0.21521712790272574
2211	23	-1.8448540585464244
2211	24	-0.6847902412966462
2211	25	-0.3251738662880541
2212	1	0.048475841486439464
2212	2	-1.5232670653085307
2212	3	-0.23665193677632956
2212	4	0.5108371445903543
2212	5	0.3331175214261677
2212	6	0.40817665024493266
2212	7	0.3549422981315331
2212	8	0.2979828851084463
2212	9	0.7453827911698525
2212	10	0.656268452092128
2212	11	0.30837884935348114
2212	12	0.27911383211007745
2212	13	0.2301640479352045
2212	14	0.5195499287269101
2212	15	0.2626725885608039
2212	16	0.33195740397854223
2212	17	0.6199574718783083
2212	18	0.654678995851917
2212	19	0.31794630750681563
2212	20	0.324166241813856
2212	21	0.3416320968878779
2212	22	0.3663159763514159
2212	23	0.5484908494866119
2212	24	0.33167113674945625
2212	25	0.25537563410359354
2213	1	0.224689126477724
2213	2	0.12673364369502949
2213	3	0.22750192779044198
2213	4	0.6143919297188845
2213	5	0.5019953444508332
2213	6	0.3908293701413328
2213	7	0.37231841457404613
2213	8	0.3724129150283986
2213	9	0.5492455272336514
2213	10	0.6482735109912738
2213	11	0.2941775609141146
2213	12	0.3103157825682956
2213	13	0.1936088295737477
2213	14	-1.2462588930128717
2213	15	-0.26358281864956723
2213	16	-0.04116408050136755
2213	17	0.4363186179751608
2213	18	0.2148271807710529
2213	19	0.17297076707149728
2213	20	0.19503021133220244
2213	21	0.3211459416110216
2213	22	0.36708167374319345
2213	23	0.5042822815912658
2213	24	0.2717197796848936
2213	25	0.05956221018994512
2214	1	0.3580909940190047
2214	2	0.29353897998475276
2214	3	0.4440549023949174
2214	4	0.717652101863905
2214	5	0.5505745590282203
2214	6	0.3906930133730597
2214	7	0.3361151080304339
2214	8	0.25510537790485444
2214	9	0.41853746199132047
2214	10	0.27487887683978585
2214	11	0.17720313841933688
2214	12	0.163142008511592
2214	13	0.056612747575165656
2214	14	0.24219822062314478
2214	15	0.05795349386946924
2214	16	0.22618086260050063
2214	17	0.23562458362825112
2214	18	0.45944493259232644
2214	19	0.17079928871378058
2214	20	0.34717231516336966
2214	21	0.2757281961945708
2214	22	0.34545953143730657
2214	23	0.3495534148888382
2214	24	0.38630535359629903
2214	25	0.2633088824430416
2215	1	0.3357071176908235
2215	2	0.21165928793025302
2215	3	0.2884595301349506
2215	4	0.7768268456757355
2215	5	0.6007105150977097
2215	6	0.5278923703722569
2215	7	0.4848131391358237
2215	8	0.45509877919510444
2215	9	0.7010981923105604
2215	10	0.7012815388009273
2215	11	0.38685427173549414
2215	12	0.26411155975097245
2215	13	0.18079276453960347
2215	14	0.3378895998251039
2215	15	0.24872482488737546
2215	16	0.3442325268250954
2215	17	0.5949895820762094
2215	18	0.7219092862459533
2215	19	0.34995217720715743
2215	20	-0.4456896682924736
2215	21	0.07687806115669105
2215	22	-0.6946783754752204
2215	23	0.2897332829844288
2215	24	0.18689880898617162
2215	25	0.24006510486412225
2216	1	0.11077482385818271
2216	2	-0.41169872148030606
2216	3	0.24894155148296093
2216	4	0.6650452741016721
2216	5	0.520355236187612
2216	6	0.5378594317791539
2216	7	0.4046760196713919
2216	8	0.41763029956434394
2216	9	0.7353148287038422
2216	10	0.4498484599486474
2216	11	0.2690317710160244
2216	12	-0.20032064364552582
2216	13	0.08254785474021276
2216	14	0.11942117758243853
2216	15	0.11227033676595966
2216	16	0.26194470209476606
2216	17	0.3352631908849233
2216	18	0.629288386176782
2216	19	0.30097103975079875
2216	20	-0.7662197747011419
2216	21	-1.0976659800988813
2216	22	-0.9879377408474852
2216	23	-1.5618355196343074
2216	24	-1.2795804624498146
2216	25	-1.02766792423769
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10999.0
3	1439.0
4	1210.0
5	892.0
6	1065.0
7	1437.0
8	984.0
9	2007.0
10	3865.0
11	6542.0
12	10987.0
13	16505.0
14	22114.0
15	27201.0
16	30081.0
17	32175.0
18	33026.0
19	32979.0
20	32889.0
21	33257.0
22	33914.0
23	35445.0
24	36952.0
25	39968.0
26	43428.0
27	49079.0
28	57265.0
29	68800.0
30	85295.0
31	108264.0
32	141106.0
33	189641.0
34	261396.0
35	371898.0
36	552583.0
37	902246.0
38	1805578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88299191299267	20.891186079962367	13.840849807486913	23.384972199558046
2	15.935510406176734	21.371676222470555	39.66847788027842	23.02433549107429
3	17.99309099098939	26.49370654851752	30.150751884225834	25.362450576267253
4	13.683202304905132	17.904972024869505	37.474038324655254	30.93778734557011
5	13.66034329114744	37.84045711072203	34.82945723729353	13.669742360836997
6	31.107096875047496	38.206715480952695	17.438392803744975	13.247794840254832
7	25.953192863498657	33.09334582913232	23.39699532354987	17.55646598381915
8	24.223511623265363	36.43529900199412	21.110470819585654	18.230718555154862
9	25.062286940369948	17.116463057162655	21.708464352051983	36.11278565041541
10	15.396081460568775	29.912400941624718	33.471867954548955	21.219649643257554
11	32.14203063874014	25.461969297337905	23.910618955897995	18.48538110802396
12	21.275980908843277	27.314704059436817	30.990857088717032	20.41845794300287
13	26.612201293379954	24.205499538557802	26.662163732754625	22.52013543530762
14	21.216373105707405	23.549033689008716	27.650657820862197	27.583935384421682
15	22.955846517742515	30.788213761062355	24.06342967778771	22.19251004340742
16	22.43032479576937	30.703539997632106	25.529006669560754	21.337128537037767
17	21.023940754007597	29.919585473897257	27.743899911040987	21.312573861054155
18	20.987243522489816	29.73133953671227	28.523958163111445	20.757458777686466
19	21.704682256621368	28.714457412058046	28.78593094523698	20.794929386083606
20	22.7323651640588	28.34432557069463	28.084544926108723	20.83876433913785
21	23.121972730542275	28.594221831120752	27.305225349849604	20.978580088487362
22	22.268076913819005	28.121314191032877	28.4138310394588	21.19677785568932
23	22.537934081888693	28.518657334711563	27.715748857834978	21.22765972556477
24	22.336881244340145	27.805802834500483	28.054821628621195	21.802494292538178
25	22.658350466355635	28.051784448918532	27.636092830296356	21.653772254429477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15662.0
1	15662.0
2	13405.5
3	11149.0
4	11149.0
5	11149.0
6	11422.0
7	11695.0
8	11695.0
9	11695.0
10	15692.5
11	19690.0
12	19690.0
13	19690.0
14	30865.5
15	42041.0
16	42041.0
17	42041.0
18	67565.5
19	93090.0
20	93090.0
21	93090.0
22	135563.5
23	178037.0
24	178037.0
25	178037.0
26	232493.0
27	286949.0
28	286949.0
29	286949.0
30	347126.5
31	407304.0
32	407304.0
33	407304.0
34	460613.5
35	513923.0
36	513923.0
37	513923.0
38	552980.5
39	592038.0
40	592038.0
41	592038.0
42	596989.0
43	601940.0
44	601940.0
45	601940.0
46	585247.5
47	568555.0
48	568555.0
49	568555.0
50	546561.0
51	524567.0
52	524567.0
53	524567.0
54	480977.5
55	437388.0
56	437388.0
57	437388.0
58	379841.5
59	322295.0
60	322295.0
61	322295.0
62	270282.5
63	218270.0
64	218270.0
65	218270.0
66	172641.0
67	127012.0
68	127012.0
69	127012.0
70	95926.5
71	64841.0
72	64841.0
73	64841.0
74	46987.0
75	29133.0
76	29133.0
77	29133.0
78	20670.5
79	12208.0
80	12208.0
81	12208.0
82	8453.0
83	4698.0
84	4698.0
85	4698.0
86	3127.0
87	1556.0
88	1556.0
89	1556.0
90	967.0
91	378.0
92	378.0
93	378.0
94	230.0
95	82.0
96	82.0
97	82.0
98	46.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15682920996154598
2	0.29829804708888485
3	0.3613522792354507
4	0.3901062678188192
5	0.3969112473330774
6	0.3559633648224254
7	0.35944452486295636
8	0.37586694652308816
9	0.36270934162413226
10	0.3948068172520785
11	0.31135731413358847
12	0.3597592059965637
13	0.32854677105688807
14	0.3392852647412377
15	0.3785810713004512
16	0.32868444405284125
17	0.37875807943810536
18	0.34607057668464547
19	0.3599362141342178
20	0.322351486238994
21	0.36341737417474873
22	0.316372544700455
23	0.2987897363601463
24	0.33480105858733344
25	0.3306708687087374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5084512.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.01266489997636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45330575151078	60.09694941878815
2	11.231074918026778	16.175592690767836
3	2.700427492875789	5.833949403934425
4	0.979878111455147	2.8225453633216473
5	0.46352475860409637	1.6689826557099612
6	0.2698368433734627	1.1659002107712346
7	0.18906205473003523	0.9530403674802512
8	0.12947160007508615	0.7458875960216748
9	0.09185976162069147	0.5953559608244308
>10	0.4515242115200272	5.7139115882934925
>50	0.027385818947677942	1.3536432106703191
>100	0.011553846104529471	1.3598910485860844
>500	5.200456752030099E-4	0.26540116364379285
>1k	4.6530253195395263E-4	0.7334633021428939
>5k	1.0948294869172367E-4	0.5154860190437685
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	7343	0.14441897275490745	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6737	0.13250042481953037	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6316	0.12422037749148787	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5757	0.11322620538608229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.9667570850457233E-5	0.0	0.0	0.0
5	0.0	3.9335141700914466E-5	0.0	0.0	0.0
6	0.0	3.9335141700914466E-5	0.0	1.9667570850457233E-5	0.0
7	0.0	3.9335141700914466E-5	0.0	1.9667570850457233E-5	0.0
8	0.0	3.9335141700914466E-5	0.0	1.9667570850457233E-5	0.0
9	0.0	3.9335141700914466E-5	0.0	5.9002712551371695E-5	0.0
10	0.0	3.9335141700914466E-5	0.0	5.9002712551371695E-5	0.0
11	0.0	3.9335141700914466E-5	0.0	7.867028340182893E-5	0.0
12	0.0	3.9335141700914466E-5	0.0	7.867028340182893E-5	0.0
13	0.0	3.9335141700914466E-5	0.0	7.867028340182893E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4125	0.0	11.200884	1
GTGGTAT	1795	0.0	10.221929	1
TGGTATC	1740	0.0	9.942451	2
GTCCTAA	420	0.0	9.733278	1
TCTAGAC	435	0.0	9.178101	3
CGGTCGT	85	0.0074494034	8.939741	10
GTACCGT	150	2.7087372E-6	8.865768	6
GTATAGG	570	0.0	8.672982	1
TAGGACG	145	1.6769305E-5	8.522859	4
GTATTAT	805	0.0	8.503087	1
CTAGGAC	465	0.0	8.381537	3
ACACCGC	125	2.7484845E-4	8.359153	6
GCACCGT	160	6.465765E-6	8.311658	6
CTGTCGC	425	0.0	8.269832	9
GTATCAA	7295	0.0	8.197196	1
GTCGTAG	140	1.031226E-4	8.148791	1
CCGGTCG	130	4.1929164E-4	8.037726	9
GTCCTAG	500	0.0	7.9858155	1
CTAGTAC	250	2.05182E-9	7.9849477	3
TGTCGCC	430	0.0	7.952211	10
>>END_MODULE