FastQCFastQC Report
Wed 25 May 2016
SRR1295194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084512
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174090.34239274093560995No Hit
GGTATCAACGCAGAGTACTTTTTTT150530.2960559440119327No Hit
TATCAACGCAGAGTACTTTTTTTTT99990.19665604093372185No Hit
GTGGTATCAACGCAGAGTACATGGG79200.15576716113562128No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62920.1237483557910769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.012.3537991
AGCGTCA4750.011.001553
GTGGTAT18850.010.9892581
TGGTATC18500.010.5284062
CATACCG750.002646464910.1346615
CGCGACT750.002650787110.13266717
GCGCGAC856.6030177E-410.058063516
GCAGCGT5000.09.6922231
ACCGTTC800.004521989.4990958
CAGCGTC5600.09.1619142
TTAGTAC2950.09.0182193
ACGGGTC1304.3662076E-58.7703795
GTGTAAG6850.08.739231
GGACCGG1107.143547E-48.6370716
GTATTAG6100.08.5675381
ACCGTCC1000.00290925618.5491878
CTACGAC1451.6879152E-58.5184413
TAGTACT5000.08.3610124
GTATCAA87450.08.2363081
TAGGACG1050.00451056758.1438444
Produced by FastQC (version 0.11.5)