FastQCFastQC Report
Wed 25 May 2016
SRR1295193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501318
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA191831.277743955644307No Hit
GTACATAAGCAGTGGTATCAACGCA175351.1679737404067625No Hit
GCTTATGTACTCTGCGTTGATACCA108340.7216325921623533No Hit
GTATCAACGCAGAGTACTTTTTTTT105340.7016501500681401No Hit
GGTATCAACGCAGAGTACTTTTTTT73270.48803784408100087No Hit
CTTATGTACTCTGCGTTGATACCAC72230.4811105974883403No Hit
TATCAACGCAGAGTACTTTTTTTTT63080.4201641491009899No Hit
GTGGTATCAACGCAGAGTACATGGG61590.4102395361941974No Hit
GAGTACATAAGCAGTGGTATCAACG46370.3088619466362223No Hit
TATGTACTCTGCGTTGATACCACTG41600.2770898637064233No Hit
GCGTTGATACCACTGCTTATGTACT36770.24491813193474No Hit
GTACTGGTTCACTATCGGTCAGTCA33580.22367013517455994No Hit
CCCATGTACTCTGCGTTGATACCAC32720.21794183510755216No Hit
CATAAGCAGTGGTATCAACGCAGAG31170.20761757335887535No Hit
ATACAGGGTGACAGCCCCGTACACA27610.18390507540707565No Hit
CTGCTTATGTACTCTGCGTTGATAC27200.18117414165419984No Hit
ACGCAGAGTACATAAGCAGTGGTAT26800.1785098160416381No Hit
GGATACCACGTGTCCCGCCCTACTC25190.167785905451077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24910.16592087752228374No Hit
ACTCTGCGTTGATACCACTGCTTAT24640.16412245773380457No Hit
GTATCAACGCAGAGTACATAAGCAG24390.16245725422595347No Hit
TATCAACGCAGAGTACATAAGCAGT22980.15306550644167324No Hit
GGTATCAACGCAGAGTACATGGGGG21250.14154229816734362No Hit
GGTATCAACGCAGAGTACATAAGCA20540.1368131202050465No Hit
CATGTACTCTGCGTTGATACCACTG20000.13321628062808813No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17830.11876231417994056No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN17000.1132338385338749No Hit
AAGCAGTGGTATCAACGCAGAGTAC16060.10697267334435477No Hit
GTATTTAGCCTTGGAGGATGGTCCC15410.10264314422394188No Hit
GTGGTATCAACGCAGAGTACATAAG15070.1003784674532644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG250.00600852819.0128151
CGTAGGA250.006015498719.0092
TTAGCGA250.0060364518.9975613
CGCTTGG402.7700863E-416.61786519
CCAATTA807.727067E-915.43551714
ATTACCA755.8636942E-815.19601617
GGTAGAC456.736178E-414.7852713
CTAGACC1006.530172E-1014.258184
GAAAATC400.0052881714.24626517
CGATCAC759.718933E-713.92735419
GACCTAT551.9616549E-413.8168697
TCTTGCA707.2969015E-613.5651518
GTCGTAC500.001491901313.308971
GCCTCGA500.001504359913.294291519
GCGCTTG500.001504738813.29384818
CCGGCTG802.0021434E-613.060822514
ACAGGCC802.0021434E-613.06082258
GCACATC751.4804484E-512.6650413
ATCGCCG751.4850346E-512.66123119
CAATTAC1001.4446778E-712.34841315
Produced by FastQC (version 0.11.5)